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. 1985 Jul 1;229(1):161–166. doi: 10.1042/bj2290161

The stoichiometry of the exchange catalysed by the mitochondrial calcium/sodium antiporter.

M D Brand
PMCID: PMC1145162  PMID: 3929768

Abstract

Rat heart mitochondria respiring on succinate in the presence of Ruthenium Red (to inhibit uptake on the Ca2+ uniporter) released Ca2+ on the calcium/sodium antiporter until a steady state was reached. Addition of the ionophore A23187 (which catalyses Ca2+/2H+ exchange) did not perturb this steady state. Thermodynamic analysis showed that if a Ca2+/nNa+ exchange with any value of n other than 2 was at equilibrium, addition of A23187 would cause an obvious change in extramitochondrial free [Ca2+]. Therefore the endogenous calcium/sodium antiporter of mitochondria catalyses electroneutral Ca2+/2Na+ exchange.

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Selected References

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