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. 1985 Jul 1;229(1):167–171. doi: 10.1042/bj2290167

Inactivation of rabbit muscle phosphoglycerate mutase by limited proteolysis with thermolysin.

N C Price, D Duncan, J W McAlister
PMCID: PMC1145163  PMID: 2994629

Abstract

Rabbit muscle phosphoglycerate mutase is inactivated by proteolysis with thermolysin. Inactivation is correlated with the breakage of one (or a few) bond(s) near one end of the polypeptide chain. There is no change in the overall conformation, quaternary structure or binding to Cibacron Blue on proteolysis. The possible analogy with the existence of a flexible tail in the yeast enzyme is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beissner R. S., Quiocho F. A., Rudolph F. B. Dinucleotide fold proteins. Interaction of arabinose binding protein with Cibacron Blue 3G-A. J Mol Biol. 1979 Nov 15;134(4):847–850. doi: 10.1016/0022-2836(79)90490-x. [DOI] [PubMed] [Google Scholar]
  2. Chang C. T., Wu C. S., Yang J. T. Circular dichroic analysis of protein conformation: inclusion of the beta-turns. Anal Biochem. 1978 Nov;91(1):13–31. doi: 10.1016/0003-2697(78)90812-6. [DOI] [PubMed] [Google Scholar]
  3. Chen Y. H., Yang J. T., Chau K. H. Determination of the helix and beta form of proteins in aqueous solution by circular dichroism. Biochemistry. 1974 Jul 30;13(16):3350–3359. doi: 10.1021/bi00713a027. [DOI] [PubMed] [Google Scholar]
  4. Fothergill L. A., Harkins R. N. The amino acid sequence of yeast phosphoglycerate mutase. Proc R Soc Lond B Biol Sci. 1982 Apr 22;215(1198):19–44. doi: 10.1098/rspb.1982.0026. [DOI] [PubMed] [Google Scholar]
  5. Girg R., Rudolph R., Jaenicke R. Limited proteolysis of porcine-muscle lactic dehydrogenase by thermolysin during reconstitution yields dimers. Eur J Biochem. 1981 Oct;119(2):301–305. doi: 10.1111/j.1432-1033.1981.tb05608.x. [DOI] [PubMed] [Google Scholar]
  6. Haggarty N. W., Fothergill L. A. Amino acid sequences of active-site histidine peptides from rabbit muscle phosphoglycerate mutase. FEBS Lett. 1980 Jan 1;109(1):18–20. doi: 10.1016/0014-5793(80)81301-9. [DOI] [PubMed] [Google Scholar]
  7. Hermann R., Rudolph R., Jaenicke R., Price N. C., Scobbie A. The reconstitution of denatured phosphoglycerate mutase. J Biol Chem. 1983 Sep 25;258(18):11014–11019. [PubMed] [Google Scholar]
  8. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  9. Price N. C., Stevens E. Distinction between cofactor-dependent and -independent phosphoglycerate mutases by chromatography on Cibacron Blue-Sepharose. Biosci Rep. 1983 Sep;3(9):857–861. doi: 10.1007/BF01133784. [DOI] [PubMed] [Google Scholar]
  10. Price N. C., Stevens E. The refolding of denatured rabbit muscle pyruvate kinase. Biochem J. 1983 Mar 1;209(3):763–770. doi: 10.1042/bj2090763. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sasaki R., Sugimoto R., Chiba H. Yeast phosphoglyceric acid mutase-modifying enzyme. Arch Biochem Biophys. 1966 Jul;115(1):53–61. doi: 10.1016/s0003-9861(66)81037-8. [DOI] [PubMed] [Google Scholar]
  12. Schmid F., Blaschek H. An early intermediate in the folding of ribonuclease A is protected against cleavage by pepsin. Biochemistry. 1984 May 8;23(10):2128–2133. doi: 10.1021/bi00305a004. [DOI] [PubMed] [Google Scholar]
  13. Sedmak J. J., Grossberg S. E. A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250. Anal Biochem. 1977 May 1;79(1-2):544–552. doi: 10.1016/0003-2697(77)90428-6. [DOI] [PubMed] [Google Scholar]
  14. Smith D. D., Coggins J. R. Isolation of a bifunctional domain from the pentafunctional arom enzyme complex of Neurospora crassa. Biochem J. 1983 Aug 1;213(2):405–415. doi: 10.1042/bj2130405. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wetlaufer D. B. Folding of protein fragments. Adv Protein Chem. 1981;34:61–92. doi: 10.1016/s0065-3233(08)60518-5. [DOI] [PubMed] [Google Scholar]
  16. Winn S. I., Watson H. C., Harkins R. N., Fothergill L. A. Structure and activity of phosphoglycerate mutase. Philos Trans R Soc Lond B Biol Sci. 1981 Jun 26;293(1063):121–130. doi: 10.1098/rstb.1981.0066. [DOI] [PubMed] [Google Scholar]

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