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. 1978 May;31(5):447–453. doi: 10.1136/jcp.31.5.447

Urinary excretion of glycosaminoglycans in disseminated neoplasm

Gerald Manley 1, Lynne Bower 1, Anna Anson 1
PMCID: PMC1145302  PMID: 649771

Abstract

Urinary glycosaminoglycan excretion was studied in 24 cases of disseminated neoplasm, 12 of which had unequivocal evidence of skeletal involvement. Urinary hydroxyproline, cetylpyridinium chloride (CPC)-precipitable uronic acid, and CPC-precipitable hexosamine were expressed as a ratio to urinary creatinine. Glycosaminoglycans contained in urine concentrated × 1000 by vacuum-dialysis were separated by electrophoresis on cellulose acetate and stained with alcian blue. Of the 12 cases with clear evidence of skeletal involvement, eight (66%) showed elevation of serum alkaline phosphatase, five (42%) showed elevation of urinary hydroxyproline, and three (25%) showed elevation of urinary uronic acid. It is concluded that urinary uronic acid is not a sensitive index of skeletal involvement in disseminated neoplasm.

The most striking feature of the study was the identification of a well-defined fraction indist inguishable from hyaluronic acid in seven (58%) of the cases with evidence of skeletal involvement. Hyaluronic acid is not normally identifiable in adult human urine. The hyaluronic acid excretors showed more consistent biochemical evidence of bone disease (elevation of serum alkaline phosphatase and urinary hydroxyproline) than the non-excretors. The possibility that the urinary hyaluronic acid is derived from degradation of skeletal hyaluronic acid is discussed. An alternative explanation is that the hyaluronic acid is derived from neoplastic cells as part of a reversion of glycosaminoglycan synthesis to a more `fetal' state, a glycosaminoglycan counterpart of the production of oncofetal antigens by neoplastic cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BITTER T., MUIR H. M. A modified uronic acid carbazole reaction. Anal Biochem. 1962 Oct;4:330–334. doi: 10.1016/0003-2697(62)90095-7. [DOI] [PubMed] [Google Scholar]
  2. BOAS N. F. Method for the determination of hexosamines in tissues. J Biol Chem. 1953 Oct;204(2):553–563. [PubMed] [Google Scholar]
  3. Bonadonna G., Merlino M. J., Myers W. P., Sonenberg M. Urinary hydroxyproline and calcium metabolism in patients with cancer. N Engl J Med. 1966 Aug 11;275(6):298–305. doi: 10.1056/NEJM196608112750603. [DOI] [PubMed] [Google Scholar]
  4. Engfeldt B., Hjerpe A. Glycosaminoglycans and proteoglycans of human bone tissue at different stages of mineralization. Acta Pathol Microbiol Scand A. 1976 Jan;84(1):95–106. doi: 10.1111/j.1699-0463.1976.tb00115.x. [DOI] [PubMed] [Google Scholar]
  5. FEDORKO M. E., MORSE S. I. ISOLATION, CHARACTERIZATION, AND DISTRIBUTION OF ACID MUCOPOLYSACCHARIDES IN RABBIT LEUCOCYTES. J Exp Med. 1965 Jan 1;121:39–48. doi: 10.1084/jem.121.1.39. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Goverde B. C., Veenkamp F. J. Routine assay of total urinary hydroxyproline based on resin-catalysed hydrolysis. Clin Chim Acta. 1972 Oct;41:29–40. doi: 10.1016/0009-8981(72)90492-5. [DOI] [PubMed] [Google Scholar]
  7. Hosley H. F., Taft E. G., Olson K. B., Gates S., Beebe R. T. Hydroxyproline excretion in malignant neoplastic disease. Arch Intern Med. 1966 Dec;118(6):565–571. [PubMed] [Google Scholar]
  8. MANLEY G. CHANGES IN VASCULAR MUCOPOLYSACCHARIDES WITH AGE AND BLOOD PRESSURE. Br J Exp Pathol. 1965 Apr;46:125–134. [PMC free article] [PubMed] [Google Scholar]
  9. Manley G., Severn M., Hawksworth J. Excretion patterns of glycosaminoglycans and glycoproteins in normal human urine. J Clin Pathol. 1968 May;21(3):339–345. doi: 10.1136/jcp.21.3.339. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Manley G., Williams U. Urinary excretion of glycosaminoglycans in the various forms of gargoylism. J Clin Pathol. 1969 Jan;22(1):67–75. doi: 10.1136/jcp.22.1.67. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Morgenstern S., Kessler G., Auerbach J., Flor R. V., Klein B. An automated p-nitrophenylphosphate serum alkaline phosphatase procedure for the AutoAnalyzer. Clin Chem. 1965 Sep;11(9):876–888. [PubMed] [Google Scholar]
  12. Motomiya M., Endo M., Arai H., Yokosawa A., Sato H., Konno K. Biochemical characterization of hyaluronic acid from a case of benign, localized, pleural mesothelioma. Am Rev Respir Dis. 1975 Jun;111(6):775–780. doi: 10.1164/arrd.1975.111.6.775. [DOI] [PubMed] [Google Scholar]
  13. Ohya T., Kaneko Y. Novel hyaluronidase from streptomyces. Biochim Biophys Acta. 1970 Mar 18;198(3):607–609. doi: 10.1016/0005-2744(70)90139-7. [DOI] [PubMed] [Google Scholar]
  14. PLATT W. D., DOOLITTLE L. H., HARTSHORN J. W. URINARY HYDROXYPROLINE EXCRETION IN METASTATIC CANCER OF BONE. N Engl J Med. 1964 Aug;271:287–290. doi: 10.1056/NEJM196408062710604. [DOI] [PubMed] [Google Scholar]
  15. Preston B. N., Davies M., Ogston A. G. The composition and physicochemical properties of hyaluronic acids prepared from ox synovial fluid and from a case of mesothelioma. Biochem J. 1965 Aug;96(2):449–474. doi: 10.1042/bj0960449. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sobocinski P. Z., Canterbury W. J., Jurgens K. H. Improved continuous-flow method for detemination of total serum hexosamines. Clin Chem. 1976 Aug;22(8):1394–1396. [PubMed] [Google Scholar]
  17. Takeuchi J., Sobue M., Sato E., Shamoto M., Miura K. Variation in glycosaminoglycan components of breast tumors. Cancer Res. 1976 Jul;36(7 Pt 1):2133–2139. [PubMed] [Google Scholar]
  18. Tokunaga M., Futami T., Wakamatsu E., Endo M., Yosizawa Z. Werner's syndrome as "hyaluronuria". Clin Chim Acta. 1975 Jul 9;62(1):89–96. doi: 10.1016/0009-8981(75)90283-1. [DOI] [PubMed] [Google Scholar]
  19. Woodhead-Galloway J., Hukins D. W. Molecular biology of cartilage. Endeavour. 1976 May;35(125):73–78. doi: 10.1016/0160-9327(76)90031-4. [DOI] [PubMed] [Google Scholar]

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