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Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1978 Jun;31(6):507–520. doi: 10.1136/jcp.31.6.507

Enzyme immunoassays with special reference to ELISA techniques.

A Voller, A Bartlett, D E Bidwell
PMCID: PMC1145337  PMID: 78929

Abstract

In this review outlines are given on various types of enzyme immunoassay. The applications to such enzyme immunoassays, especially ELISA, are dealth with in detail. It is concluded that these techniques have high sensitivity and will be suitable in due course as routine laboratory tests.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ahlstedt S., Hanson L. A., Wadsworth C. A Clq immunosorbent assay compared with thin-layer gel filtration for measuring IgG aggregates. Scand J Immunol. 1976;5(3):293–298. doi: 10.1111/j.1365-3083.1976.tb00281.x. [DOI] [PubMed] [Google Scholar]
  2. Avrameas S. Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies. Immunochemistry. 1969 Jan;6(1):43–52. doi: 10.1016/0019-2791(69)90177-3. [DOI] [PubMed] [Google Scholar]
  3. Avrameas S., Guilbert B. Dosage enzymo-immunologiaue de protéines à l'aide d'immunoabsorbants et d'antigènes marqués aux enzymes. C R Acad Sci Hebd Seances Acad Sci D. 1971 Dec 20;273(25):2705–2707. [PubMed] [Google Scholar]
  4. Avrameas S., Ternynck T. Peroxidase labelled antibody and Fab conjugates with enhanced intracellular penetration. Immunochemistry. 1971 Dec;8(12):1175–1179. doi: 10.1016/0019-2791(71)90395-8. [DOI] [PubMed] [Google Scholar]
  5. Barbour H. M. Development of an enzyme immunoassay for human placental lactogen using labelled antibodies. J Immunol Methods. 1976;11(1):15–23. doi: 10.1016/0022-1759(76)90014-4. [DOI] [PubMed] [Google Scholar]
  6. Bartlett A., Bidwell D. E., Voller A. Preliminary studies on the application of enzyme immunoassay in the detection of antibodies in onchocerciasis. Tropenmed Parasitol. 1975 Sep;26(3):370–374. [PubMed] [Google Scholar]
  7. Bartlett A., Dormandy K. M., Hawkey C. M., Stableforth P., Voller A. Factor-VIII-related antigen: measurement by enzyme immunoassay. Br Med J. 1976 Apr 24;1(6016):994–996. doi: 10.1136/bmj.1.6016.994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Belanger L., Hamel D., Dufour D., Pouliot M. Double-antibody enzyme immunoassay applied to human alpha 1-fetoprotein. Clin Chem. 1976 Feb;22(2):198–204. [PubMed] [Google Scholar]
  9. Belanger L., Sylvestre C., Dufour D. Enzyme-linked immunoassay for alpha-fetoprotein by competitive and sandwich procedures. Clin Chim Acta. 1973 Sep 28;48(1):15–18. doi: 10.1016/0009-8981(73)90211-8. [DOI] [PubMed] [Google Scholar]
  10. Bidwell D. E., Bartlett A., Voller A. Enzyme immunoassays for viral diseases. J Infect Dis. 1977 Oct;136 (Suppl):S274–S278. doi: 10.1093/infdis/136.supplement_2.s274. [DOI] [PubMed] [Google Scholar]
  11. Bos H. J., Eijk A. A., Steerenberg P. A. Letter: Application of ELISA--enzyme linked immunosorbent assay--in the sero-diagnosis of amoebiasis. Trans R Soc Trop Med Hyg. 1975;69(4):440–440. doi: 10.1016/0035-9203(75)90202-3. [DOI] [PubMed] [Google Scholar]
  12. Bullock S. L., Walls K. W. Evaluation of some of the parameters of the enzyme-linked immunospecific assay. J Infect Dis. 1977 Oct;136 (Suppl):S279–S285. doi: 10.1093/infdis/136.supplement_2.s279. [DOI] [PubMed] [Google Scholar]
  13. Cais M., Dani S., Eden Y., Gandolfi O., Horn M., Isaacs E. E., Josephy Y., Saar Y., Slovin E., Snarsky L. Metalloimmunoassay. Nature. 1977 Dec 8;270(5637):534–535. doi: 10.1038/270534a0. [DOI] [PubMed] [Google Scholar]
  14. Carlsson H. E., Hurvell B., Lindberg A. A. Enzyme-linked immunosorbent assay (ELISA) for titration of antibodies against Brucella abortus and Yersinia enterocolitica. Acta Pathol Microbiol Scand C. 1976 Jun;84(3):168–176. doi: 10.1111/j.1699-0463.1976.tb00016.x. [DOI] [PubMed] [Google Scholar]
  15. Carlsson H. E., Lindberg A. A., Hammarström S., Ljunggren A. Quantitation of Salmonella O-antibodies in human sera by enzyme-linked immunosorbent assay (ELISA). Int Arch Allergy Appl Immunol. 1975;48(4):485–494. doi: 10.1159/000231336. [DOI] [PubMed] [Google Scholar]
  16. Carlsson H. E., Lindberg A. A., Hammarström S. Titration of antibodies to salmonella O antigens by enzyme-linked immunosorbent assay. Infect Immun. 1972 Nov;6(5):703–708. doi: 10.1128/iai.6.5.703-708.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Casali P., Bossus A., Carpentier N. A., Lambert P. H. Solid-phase enzyme immunoassay or radioimmunoassay for the detection of immune complexes based on their recognition by conglutinin: conglutinin-binding test. A comparative study with 125I-labelled Clq binding and Raji-cell RIA tests. Clin Exp Immunol. 1977 Aug;29(2):342–354. [PMC free article] [PubMed] [Google Scholar]
  18. Castellano G. A., Hazzard G. T., Madden D. L., Sever J. L. Comparison of the enzyme-linked immunosorbent assay and the indirect hemagglutination test for detection of antibody to cytomegalovirus. J Infect Dis. 1977 Oct;136 (Suppl):S337–S340. doi: 10.1093/infdis/136.supplement_2.s337. [DOI] [PubMed] [Google Scholar]
  19. Clark M. F., Adams A. N. Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J Gen Virol. 1977 Mar;34(3):475–483. doi: 10.1099/0022-1317-34-3-475. [DOI] [PubMed] [Google Scholar]
  20. Comoglio S., Celada F. An immuno-enzymatic assay of cortisol using E. coli beta-galactosidase as label. J Immunol Methods. 1976 Mar;10(2-3):161–170. doi: 10.1016/0022-1759(76)90167-8. [DOI] [PubMed] [Google Scholar]
  21. Deelder A. M., Ruttenberg E. J., Kornelis D., Steerenberg P. A. Schistosoma mansoni: comparison of the immunoperoxidase techniques, DASS and ELISA, for human diagnosis. Exp Parasitol. 1977 Feb;41(1):133–140. doi: 10.1016/0014-4894(77)90139-4. [DOI] [PubMed] [Google Scholar]
  22. Dray F., Andrieu J. M., Renaud F. Enzyme immunoassay of progesterone at the picogram level using beta-galactosidase as label. Biochim Biophys Acta. 1975 Sep 22;403(1):131–138. doi: 10.1016/0005-2744(75)90016-9. [DOI] [PubMed] [Google Scholar]
  23. Engvall E. Determination of antibodies to D.N.A. by ELISA. Lancet. 1976 Dec 25;2(8000):1410–1410. doi: 10.1016/s0140-6736(76)91949-8. [DOI] [PubMed] [Google Scholar]
  24. Engvall E., Jonsson K., Perlmann P. Enzyme-linked immunosorbent assay. II. Quantitative assay of protein antigen, immunoglobulin G, by means of enzyme-labelled antigen and antibody-coated tubes. Biochim Biophys Acta. 1971 Dec 28;251(3):427–434. doi: 10.1016/0005-2795(71)90132-2. [DOI] [PubMed] [Google Scholar]
  25. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry. 1971 Sep;8(9):871–874. doi: 10.1016/0019-2791(71)90454-x. [DOI] [PubMed] [Google Scholar]
  26. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol. 1972 Jul;109(1):129–135. [PubMed] [Google Scholar]
  27. Eriksson N. E., Ahlstedt S. Diagnosis of reaginic allergy with house dust, animal dander and pollen allergens in adult patients. V. A comparison between the enzyme-linked immunosorbent assay (ELISA), provocation tests, skin tests and RAST. Int Arch Allergy Appl Immunol. 1977;54(1):88–95. doi: 10.1159/000231811. [DOI] [PubMed] [Google Scholar]
  28. Felgner P. Serologic diagnosis of extraintestinal amebiasis: a comparison of stick-ELISA and other immunological tests. Tropenmed Parasitol. 1977 Dec;28(4):491–493. [PubMed] [Google Scholar]
  29. Fuccaro-Decaens C., Meriadec B., Buffe D., Burtin P. Etude comparative de techniques sensibles de dosage immunologique: le dosage radioimmunologique et le dosage enzymoimmunologique, appliqués à la détection de l'alpha2H-globuline sérique. Ann Immunol (Paris) 1974 Aug-Sep;125 100(5):747–761. [PubMed] [Google Scholar]
  30. Galen R. S., Forman D. W., O'Brien J. E., Brown P. A. Enzyme-multiplied immunoassay. Lancet. 1976 Oct 16;2(7990):852–853. doi: 10.1016/s0140-6736(76)91237-x. [DOI] [PubMed] [Google Scholar]
  31. Gilman S. C., Docherty J. J. Detection of antibodies specific for herpes simplex virus in human sera by the enzyme-linked immunosorbent assay. J Infect Dis. 1977 Oct;136 (Suppl):S286–S293. doi: 10.1093/infdis/136.supplement_2.s286. [DOI] [PubMed] [Google Scholar]
  32. Goldblum R. M., Ahlstedt S., Carlsson B., Hanson L. A., Jodal U., Lidin-Janson G., Sohl-Akerlund A. Antibody-forming cells in human colostrum after oral immunisation. Nature. 1975 Oct 30;257(5529):797–798. doi: 10.1038/257797a0. [DOI] [PubMed] [Google Scholar]
  33. Grauballe P. C., Vestergaard B. F. ELISA for herpes simplex virus type 2 antibodies. Lancet. 1977 Nov 12;2(8046):1038–1039. doi: 10.1016/s0140-6736(77)92948-8. [DOI] [PubMed] [Google Scholar]
  34. Haimovich J., Hurwitz E., Novik N., Sela M. Use of protein-bacteriophage conjugates for detection and quantitation of proteins. Biochim Biophys Acta. 1970 Apr 28;207(1):125–129. doi: 10.1016/0005-2795(70)90142-x. [DOI] [PubMed] [Google Scholar]
  35. Halbert S. P., Anken M. Detection of hepatitis B surface antigen (HBS Ag) with use of alkaline phosphatase-labeled antibody to HBS Ag. J Infect Dis. 1977 Oct;136 (Suppl):S318–S323. doi: 10.1093/infdis/136.supplement_2.s318. [DOI] [PubMed] [Google Scholar]
  36. Halle S., Dasch G. A., Weiss E. Sensitive enzyme-linked immunosorbent assay for detection of antibodies against typhus rickettsiae, Rickettsia prowazekii and Rickettsia typhi. J Clin Microbiol. 1977 Aug;6(2):101–110. doi: 10.1128/jcm.6.2.101-110.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Hamaguchi Y., Kato K., Fukui H., Shirakawa I., Okawa S. Enzyme-linked sandwich immunoassay of macromolecular antigens using the rabbit antibody-coupled glass rod as a solid phase. Eur J Biochem. 1976 Dec 11;71(2):459–467. doi: 10.1111/j.1432-1033.1976.tb11134.x. [DOI] [PubMed] [Google Scholar]
  38. Hamaguchi Y., Kato K., Ishikawa E., Kobayashi K., Katunuma N. Enzyme-linked sandwich immunoassay of macromolecular antigens using the rabbit antibody-loaded silicone piece as a solid phase. FEBS Lett. 1976 Oct 15;69(1):11–14. doi: 10.1016/0014-5793(76)80642-4. [DOI] [PubMed] [Google Scholar]
  39. Hammarström S., Engvall E., Johansson B. G., Svensson S., Sundblad G., Goldstein I. J. Nature of the tumor-associated determinant(s) of carcinoembryonic antigen. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1528–1532. doi: 10.1073/pnas.72.4.1528. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Herrmann J. E., Hollingdale M. R., Collins M. F., Vinson J. W. Enzyme immunoassay and radioimmunoprecipitation tests for the detection of antibodies to Rochalimaea (Rickettsia) quintana. Proc Soc Exp Biol Med. 1977 Feb;154(2):285–288. doi: 10.3181/00379727-154-39655. [DOI] [PubMed] [Google Scholar]
  41. Hoffman D. R. Estimation of serum IgE by an enzyme-linked immunosorbent assay (ELISA). J Allergy Clin Immunol. 1973 May;51(5):303–307. doi: 10.1016/0091-6749(73)90132-2. [DOI] [PubMed] [Google Scholar]
  42. Holmgren J., Svennerholm A. M. Enzyme-linked immunosorbent assays for cholera serology. Infect Immun. 1973 May;7(5):759–763. doi: 10.1128/iai.7.5.759-763.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  43. Hommel M., Kien Truong T., Bidwell D. E. Technique immuno-enzymatique (E.L.I.S.A.) appliquée au diagnostic sérologique des candidoses et aspergilloses humaines. Résultats préliminaires. Nouv Presse Med. 1976 Dec 4;5(41):2789–2791. [PubMed] [Google Scholar]
  44. Ishikawa E. Enzyme immunoassay of insulin by fluorimetry of the insulin-glucoamylase complex. J Biochem. 1973 Jun;73(6):1319–1321. doi: 10.1093/oxfordjournals.jbchem.a130209. [DOI] [PubMed] [Google Scholar]
  45. Jodal U., Ahlstedt S., Carlsson B., Hanson L. A., Lindberg U., Sohl A. Local antibodies in childhood urinary tract infection: a preliminary study. Int Arch Allergy Appl Immunol. 1974;47(4):537–546. doi: 10.1159/000231248. [DOI] [PubMed] [Google Scholar]
  46. Joyce B. G., Read G. F., Fahmy D. R. A specific enzymeimmunoassay for progesterone in human plasma. Steroids. 1977 Jun;29(6):761–770. doi: 10.1016/0039-128x(77)90120-9. [DOI] [PubMed] [Google Scholar]
  47. Kacaki J., Wolters G., Kuijpers L., Schuurs A. Specificity control in solid-phase enzyme immunoassay for HBsAg by one-step in situ blocking with human anti-HBs. J Clin Pathol. 1977 Sep;30(9):894–898. doi: 10.1136/jcp.30.9.894. [DOI] [PMC free article] [PubMed] [Google Scholar]
  48. Kato K., Fukui H., Hamaguchi Y., Ishikawa E. Enzyme-linked immunoassay: conjugation of the Fab' fragment of rabbit IgG with beta-D-galactosidase from E. coli and its use for immunoassay. J Immunol. 1976 Jun;116(6):1554–1560. [PubMed] [Google Scholar]
  49. Kato K., Hamaguchi Y., Fukui H., Ishikawa E. Coupling Fab fragment of rabbit anti-human IgG antibody to beta-D-galactosidase and a highly sensitive immunoassay of human IgG. FEBS Lett. 1975 Aug 15;56(2):370–372. doi: 10.1016/0014-5793(75)81130-6. [DOI] [PubMed] [Google Scholar]
  50. Kato K., Hamaguchi Y., Fukui H., Ishikawa E. Enzyme-linked immunoassay. Conjugation of rabbit anti-(human immunoglobulin G) antibody with beta-D-galactosidase from Escherichia coli and its use for human immunoglobulin G assay. Eur J Biochem. 1976 Feb 16;62(2):285–292. doi: 10.1111/j.1432-1033.1976.tb10159.x. [DOI] [PubMed] [Google Scholar]
  51. Kirkpatrick A., Wepsic H. T., Nakamura R. M. Comparison of double-antibody radioimmunoassay with Farr-technique radioimmunoassay and double-antibody enzyme immunoassay for alpha-fetoprotein. Clin Chem. 1977 Jan;23(1):50–59. [PubMed] [Google Scholar]
  52. Kitagawa T., Aikawa T. Enzyme coupled immunoassay of insulin using a novel coupling reagent. J Biochem. 1976 Jan;79(1):233–236. doi: 10.1093/oxfordjournals.jbchem.a131053. [DOI] [PubMed] [Google Scholar]
  53. Lange W., Köhler H., Apodaca J., Ott A., Sucker U. Der Nachweis von HBs-Antigen mit dem Hepanostika-test, einem Enzymimmunoassay. Dtsch Med Wochenschr. 1977 Nov 4;102(44):1581–1585. doi: 10.1055/s-0028-1105541. [DOI] [PubMed] [Google Scholar]
  54. Lauer R. C., Erlanger B. F. An enzyme-immunoassay of antibody specific for adenosine using beta-galactosidase. Immunochemistry. 1974 Sep;11(9):533–536. doi: 10.1016/0019-2791(74)90243-2. [DOI] [PubMed] [Google Scholar]
  55. Leinikki P. O., Passila S. Quantitative, semiautomated, enzyme-linked immunosorbent assay for viral antibodies. J Infect Dis. 1977 Oct;136 (Suppl):S294–S299. doi: 10.1093/infdis/136.supplement_2.s294. [DOI] [PubMed] [Google Scholar]
  56. Leinikki P., Pässilä S. Solid phase antibody assay by means of enzyme conjugated to anti-immunoglobulin. J Clin Pathol. 1976 Dec;29(12):1116–1120. doi: 10.1136/jcp.29.12.1116. [DOI] [PMC free article] [PubMed] [Google Scholar]
  57. Leute R., Ullman E. F., Goldstein A. Spin immunoassay of opiate narcotics in urine and saliva. JAMA. 1972 Sep 11;221(11):1231–1234. [PubMed] [Google Scholar]
  58. Maiolini R., Ferrua B., Masseyeff R. Enzymoimmunoassay of human alpha-fetoprotein. J Immunol Methods. 1975 Feb;6(4):355–362. doi: 10.1016/0022-1759(75)90006-x. [DOI] [PubMed] [Google Scholar]
  59. Maiolini R., Masseyeff R. A sandwich method of enzymoimmunoassay. I. Application to rat and human alpha-fetoprotein. J Immunol Methods. 1975 Sep;8(3):223–234. doi: 10.1016/0022-1759(75)90115-5. [DOI] [PubMed] [Google Scholar]
  60. Miedema K., Boelhouwer J., Otten J. W. Determinations of proteins and hormones in serum by an immunoassay using antigen-enzyme conjugates. Clin Chim Acta. 1972 Aug;40(1):187–192. doi: 10.1016/0009-8981(72)90269-0. [DOI] [PubMed] [Google Scholar]
  61. Miles L. E., Hales C. N. Labelled antibodies and immunological assay systems. Nature. 1968 Jul 13;219(5150):186–189. doi: 10.1038/219186a0. [DOI] [PubMed] [Google Scholar]
  62. Miranda Q. R., Bailey G. D., Fraser A. S., Tenoso H. J. Solid-phase enzyme immunoassay for herpes simplex virus. J Infect Dis. 1977 Oct;136 (Suppl):S304–S310. doi: 10.1093/infdis/136.supplement_2.s304. [DOI] [PubMed] [Google Scholar]
  63. Miyai K., Ishibashi K., Kumahara Y. Enzyme-linked immunoassay of thyrotropin. Clin Chim Acta. 1976 Mar 15;67(3):263–268. doi: 10.1016/0009-8981(76)90334-x. [DOI] [PubMed] [Google Scholar]
  64. Nakane P. K., Kawaoi A. Peroxidase-labeled antibody. A new method of conjugation. J Histochem Cytochem. 1974 Dec;22(12):1084–1091. doi: 10.1177/22.12.1084. [DOI] [PubMed] [Google Scholar]
  65. Nassau E., Parsons E. R., Johnson G. D. The detection of antibodies to Mycobacterium tuberculosis by microplate enzyme-linked immunosorbent assay (ELISA). Tubercle. 1976 Mar;57(1):67–70. doi: 10.1016/0041-3879(76)90019-2. [DOI] [PubMed] [Google Scholar]
  66. Ogihara T., Miyal K., Nishi K., Ishibashi K., Kumahara Y. Enzyme-labelled immunoassay for plasma cortisol. J Clin Endocrinol Metab. 1977 Jan;44(1):91–95. doi: 10.1210/jcem-44-1-91. [DOI] [PubMed] [Google Scholar]
  67. Pesce A. J., Mendoza N., Boreisha I., Gaizutis M. A., Pollak V. E. Use of enzyme-linked antibodies to measure serum anti-DNA antibody in systemic lupus erythematosus. Clin Chem. 1974 Mar;20(3):353–359. [PubMed] [Google Scholar]
  68. Polak J. M., Wigglesworth J. S. Letter: Islet-cell hyperplasia and sudden infant death. Lancet. 1976 Sep 11;2(7985):570–571. doi: 10.1016/s0140-6736(76)91814-6. [DOI] [PubMed] [Google Scholar]
  69. Rubenstein K. E., Schneider R. S., Ullman E. F. "Homogeneous" enzyme immunoassay. A new immunochemical technique. Biochem Biophys Res Commun. 1972 May 26;47(4):846–851. doi: 10.1016/0006-291x(72)90570-0. [DOI] [PubMed] [Google Scholar]
  70. Ruitenberg E. J., Buys J. Application of the enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of human African trypanosomiasis (sleeping sickness). Am J Trop Med Hyg. 1977 Jan;26(1):31–36. doi: 10.4269/ajtmh.1977.26.31. [DOI] [PubMed] [Google Scholar]
  71. Ruitenberg E. J., Steerenberg P. A., Brosi B. J., Buys J. Reliability of the enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of Trichinella spiralis infections in conventionally raised pigs. J Immunol Methods. 1976;10(1):67–83. doi: 10.1016/0022-1759(76)90008-9. [DOI] [PubMed] [Google Scholar]
  72. Ruitenberg E. J., van Amstel J. A., Brosi B. J., Steerenberg P. A. Mechanization of the enzyme-linked immunosorbent assay (ELISA) for large scale screening of sera. J Immunol Methods. 1977;16(4):351–359. doi: 10.1016/s0022-1759(97)90005-3. [DOI] [PubMed] [Google Scholar]
  73. Russell H., Facklam R. R., Edwards L. R. Enzyme-linked immunosorbent assay for streptococcal M protein antibodies. J Clin Microbiol. 1976 May;3(5):501–505. doi: 10.1128/jcm.3.5.501-505.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  74. Saunders G. C., Clinard E. H., Bartlett M. L., Sanders W. M. Application of the indirect enzyme-labeled antibody microtest to the detection and surveillance of animal diseases. J Infect Dis. 1977 Oct;136 (Suppl):S258–S266. doi: 10.1093/infdis/136.supplement_2.s258. [DOI] [PubMed] [Google Scholar]
  75. Saunders G. C., Wilder M. E. Disease screening with enzyme-labeled antibodies. J Infect Dis. 1974 Mar;129(3):362–364. doi: 10.1093/infdis/129.3.362. [DOI] [PubMed] [Google Scholar]
  76. Schinski V. D., Clutter W. C., Murrell K. D. Enzyme- and 125I-labeled anti-immunoglobulin assays in the immunodiagnosis of schistosomiasis. Am J Trop Med Hyg. 1976 Nov;25(6):824–831. doi: 10.4269/ajtmh.1976.25.824. [DOI] [PubMed] [Google Scholar]
  77. Schmitz H., Doerr H. W., Kampa D., Vogt A. Solid-phase enzyme immunoassay for immunoglobulin M antibodies to cytomegalovirus. J Clin Microbiol. 1977 Jun;5(6):629–634. doi: 10.1128/jcm.5.6.629-634.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  78. Schuurs A. H., Van Weemen B. K. Enzyme-immunoassay. Clin Chim Acta. 1977 Nov 15;81(1):1–40. doi: 10.1016/0009-8981(77)90410-7. [DOI] [PubMed] [Google Scholar]
  79. Smith J., Holmgren J., Ahlstedt S., Hanson L. A. Local antibody production in experimental pyelonephritis: amount, avidity, and immunoglobulin class. Infect Immun. 1974 Sep;10(3):411–415. doi: 10.1128/iai.10.3.411-415.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  80. Stiffler-Rosenberg G., Fey H. Messung von Tetanus-Antitoxin mit dem Enzyme-linked immunosorbent assay (ELISA). Schweiz Med Wochenschr. 1977 Aug 6;107(31):1101–1104. [PubMed] [Google Scholar]
  81. Stimson W. H., Sinclair J. M. An immunoassay for a pregnancy-associated alpha-macroglobulin using antibody-enzyme conjugates. FEBS Lett. 1974 Oct 1;47(1):190–192. doi: 10.1016/0014-5793(74)80456-4. [DOI] [PubMed] [Google Scholar]
  82. Theakston R. D., Lloyd-Jones M. J., Reid H. A. Micro-ELISA for detecting and assaying snake venom and venom-antibody. Lancet. 1977 Sep 24;2(8039):639–641. doi: 10.1016/s0140-6736(77)92502-8. [DOI] [PubMed] [Google Scholar]
  83. Thraenhart O., Kuwert E. K. Enzyme immunoassay for demonstration of rabies-virus antibodies after immunisation. Lancet. 1977 Aug 20;2(8034):399–400. doi: 10.1016/s0140-6736(77)90321-x. [DOI] [PubMed] [Google Scholar]
  84. Ukkonen P., Koistinen V., Penttinen K. Enzyme-immunoassay in the detection of hepatitis B surface antigen. J Immunol Methods. 1977;15(4):343–353. doi: 10.1016/0022-1759(77)90096-5. [DOI] [PubMed] [Google Scholar]
  85. Van Weemen B. K., Schuurs A. H.W.M. Immunoassay using antigen-enzyme conjugates. FEBS Lett. 1971 Jun 24;15(3):232–236. doi: 10.1016/0014-5793(71)80319-8. [DOI] [PubMed] [Google Scholar]
  86. Vandervelde E. M., Cohen B. J., Cossart Y. E. An enzyme-linked immunosorbent-assay test for hepatitis B surface antigen. J Clin Pathol. 1977 Aug;30(8):714–716. doi: 10.1136/jcp.30.8.714. [DOI] [PMC free article] [PubMed] [Google Scholar]
  87. Veldkamp J., Visser A. M. Application of the enzyme-linked immunosorbent assay (ELISA) in the serodiagnosis of syphilis. Br J Vener Dis. 1975 Aug;51(4):227–231. doi: 10.1136/sti.51.4.227. [DOI] [PMC free article] [PubMed] [Google Scholar]
  88. Vestergaard B. F., Grauballe P. C., Spanggaard H. Titration of herpes simplex virus antibodies in human sera by the enzyme-linked immunosorbent assay (ELISA). Acta Pathol Microbiol Scand B. 1977 Dec;85B(6):466–468. doi: 10.1111/j.1699-0463.1977.tb02004.x. [DOI] [PubMed] [Google Scholar]
  89. Voller A., Bartlett A., Bidwell D. E., Clark M. F., Adams A. N. The detection of viruses by enzyme-linked immunosorbent assay (ELISA). J Gen Virol. 1976 Oct;33(1):165–167. doi: 10.1099/0022-1317-33-1-165. [DOI] [PubMed] [Google Scholar]
  90. Voller A., Bidwell D. E. A simple method for detecting antibodies to rubella. Br J Exp Pathol. 1975 Aug;56(4):338–339. [PMC free article] [PubMed] [Google Scholar]
  91. Voller A., Bidwell D. E., Bartlett A., Edwards R. A comparison of isotopic and enzyme-immunoassays for tropical parasitic diseases. Trans R Soc Trop Med Hyg. 1977;71(5):431–437. doi: 10.1016/0035-9203(77)90046-3. [DOI] [PubMed] [Google Scholar]
  92. Voller A., Bidwell D. E., Bartlett A. Enzyme immunoassays in diagnostic medicine. Theory and practice. Bull World Health Organ. 1976;53(1):55–65. [PMC free article] [PubMed] [Google Scholar]
  93. Voller A., Bidwell D. E., Bartlett A., Fleck D. G., Perkins M., Oladehin B. A microplate enzyme-immunoassay for toxoplasma antibody. J Clin Pathol. 1976 Feb;29(2):150–153. doi: 10.1136/jcp.29.2.150. [DOI] [PMC free article] [PubMed] [Google Scholar]
  94. Voller A., Bidwell D. E. Enzyme-immunoassays for antibodies in measles, cytomegalovirus infections and after rubella vaccination. Br J Exp Pathol. 1976 Apr;57(2):243–247. [PMC free article] [PubMed] [Google Scholar]
  95. Voller A., Bidwell D., Bartlett A. A serological study on human Trypanosoma rhodesiense infections using a micro-scale enzyme linked immunosorbent assay. Tropenmed Parasitol. 1975 Jun;26(2):247–251. [PubMed] [Google Scholar]
  96. Voller A., Bidwell D., Huldt G., Engvall E. A microplate method of enzyme-linked immunosorbent assay and its application to malaria. Bull World Health Organ. 1974;51(2):209–211. [PMC free article] [PubMed] [Google Scholar]
  97. Voller A., Draper C., Bidwell D. E., Bartlett A. Microplate enzyme-linked immunosorbent assay for chagas' disease. Lancet. 1975 Feb 22;1(7904):426–428. doi: 10.1016/s0140-6736(75)91492-0. [DOI] [PubMed] [Google Scholar]
  98. Voller A., Huldt G., Thors C., Engvall E. New serological test for malaria antibodies. Br Med J. 1975 Mar 22;1(5959):659–661. doi: 10.1136/bmj.1.5959.659. [DOI] [PMC free article] [PubMed] [Google Scholar]
  99. Wallen W. C., Mattson J. M., Levine P. H. Detection of soluble antigen of Epstein-Barr virus by the enzyme-linked immunosorbent assay. J Infect Dis. 1977 Oct;136 (Suppl):S324–S328. doi: 10.1093/infdis/136.supplement_2.s324. [DOI] [PubMed] [Google Scholar]
  100. Walls K. W., Bullock S. L., English D. K. Use of the enzyme-linked immunosorbent assay (ELISA) and its microadaptation for the serodiagnosis of toxoplasmosis. J Clin Microbiol. 1977 Mar;5(3):273–277. doi: 10.1128/jcm.5.3.273-277.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  101. Warren R. C., Bartlett A., Bidwell D. E., Richardson M. D., Voller A., White L. O. Diagnosis of invasive candidosis by enzyme immunoassay of serum antigen. Br Med J. 1977 May 7;1(6070):1183–1185. doi: 10.1136/bmj.1.6070.1183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  102. Weltman J. K., Frackelton A. R., Jr, Szaro R. P., Rotman B. A galactosidase immunosorbent test for human immunoglobulin E. J Allergy Clin Immunol. 1976 Sep;58(3):426–431. doi: 10.1016/0091-6749(76)90123-8. [DOI] [PubMed] [Google Scholar]
  103. Wolters G., Kuijpers L. P., Kacaki J., Schuurs A. H. Enzyme-linked immunosorbent assay for hepatitis B surface antigen. J Infect Dis. 1977 Oct;136 (Suppl):S311–S317. doi: 10.1093/infdis/136.supplement_2.s311. [DOI] [PubMed] [Google Scholar]
  104. Wolters G., Kuijpers L., Kacaki J., Schuurs A. Solid-phase enzyme-immunoassay for detection of hepatitis B surface antigen. J Clin Pathol. 1976 Oct;29(10):873–879. doi: 10.1136/jcp.29.10.873. [DOI] [PMC free article] [PubMed] [Google Scholar]
  105. Yolken R. H., Kim H. W., Clem T., Wyatt R. G., Kalica A. R., Chanock R. M., Kapikian A. Z. Enzyme-linked immunosorbent assay (ELISA) for detection of human reovirus-like agent of infantile gastroenteritis. Lancet. 1977 Aug 6;2(8032):263–267. doi: 10.1016/s0140-6736(77)90951-5. [DOI] [PubMed] [Google Scholar]
  106. Yorde D. E., Sasse E. A., Wang T. Y., Hussa R. O., Garancis J. C. Competitive enzyme-liked immunoassay with use of soluble enzyme/antibody immune complexes for labeling. I. Measurement of human choriogonadotropin. Clin Chem. 1976 Aug;22(8):1372–1377. [PubMed] [Google Scholar]
  107. van Weeman B. K., Schuurs A. H. The influence of heterologous combinations of antiserum and enzyme-labeled estrogen on the characteristics of estrogen enzyme-immunoassays. Immunochemistry. 1975 Aug;12(8):667–670. doi: 10.1016/0019-2791(75)90213-x. [DOI] [PubMed] [Google Scholar]
  108. van Weemen B. K., Schuurs A. H. Immunoassay using antibody--enzyme conjugates. FEBS Lett. 1974 Jul 15;43(2):215–218. doi: 10.1016/0014-5793(74)81003-3. [DOI] [PubMed] [Google Scholar]
  109. van Weemen B. K., Schuurs A. H. Immunoassay using hapten--enzyme conjugates. FEBS Lett. 1972 Jul 15;24(1):77–81. doi: 10.1016/0014-5793(72)80830-5. [DOI] [PubMed] [Google Scholar]

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