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. 1978 Dec;31(12):1161–1166. doi: 10.1136/jcp.31.12.1161

Determination of antibody levels to Candida albicans in healthy and hospitalised adults using a radioimmunoassay.

S J Cobb, D Parratt
PMCID: PMC1145524  PMID: 107198

Abstract

A radioimmunoassay for antibody to Candida albicans is described. The test used whole, killed organisms as the antigen and radiolabelled sheep anti-human globulins to quantitate different classes of antibody to C. albicans. The assay has been compared with an Ouchterlony precipitin method and found to be simpler, more rapid, and more sensitive than the latter. Results obtained from two groups of symptomless adults indicated that the range of antibody level was wider for a hospitalised group than for a group of blood transfusion donors, particularly in respect of IgG and IgA antibody. The reason for the increase of antibody in hospital patients was not clear but may have been related to antibiotic therapy. The difficulties in interpretation of Candida serology have therefore been re-assessed in the light of more detailed knowledge of the range and type of antibody to be expected in normal individuals.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Axelsen N. H. Analysis of human Candida precipitins by quantitative immunoelectrophoresis: a model for analysis of complex microbial antigen-antibody systems. Scand J Immunol. 1976;5(3):177–190. doi: 10.1111/j.1365-3083.1976.tb00268.x. [DOI] [PubMed] [Google Scholar]
  2. Axelsen N. H., Kirkpatrick C. H., Buckley R. H. Precipitins to Candida albicans in chronic mucocutaneous candidiasis studied by crossed immunoelectrophoresis with intermediate gel. Correlation with clinical and immunological findings. Clin Exp Immunol. 1974 Jul;17(3):385–394. [PMC free article] [PubMed] [Google Scholar]
  3. Chew W. H., Theus T. L. Candida precipitins. J Immunol. 1967 Feb;98(2):220–224. [PubMed] [Google Scholar]
  4. Ho Y. M., Ng M. H., Teoh-Chan C. H., Yue P. C., Huang C. T. Indirect immunofluorescence assay for antibody to germ tube of Candida albicans--a new diagnostic test. J Clin Pathol. 1976 Nov;29(11):1007–1010. doi: 10.1136/jcp.29.11.1007. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hollingdale M. R. Antibody responses in patients with farmer's lung disease to antigens from Micropolyspora faeni. J Hyg (Lond) 1974 Feb;72(1):79–89. doi: 10.1017/s002217240002324x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lehner T. Immunofluorescence study of Candida albicans in candidiasis, carriers and controls. J Pathol Bacteriol. 1966 Jan;91(1):97–104. doi: 10.1002/path.1700910114. [DOI] [PubMed] [Google Scholar]
  7. McConahey P. J., Dixon F. J. A method of trace iodination of proteins for immunologic studies. Int Arch Allergy Appl Immunol. 1966;29(2):185–189. doi: 10.1159/000229699. [DOI] [PubMed] [Google Scholar]
  8. Nielsen K. H., Parratt D., White R. G. Quantitation of antibody to particulate antigens using a radiolabelled anti-immunoglobulin reagent: application to estimation of antibody in farmer's lung syndrome. J Immunol Methods. 1973 Nov;3(3):301–313. doi: 10.1016/0022-1759(73)90025-2. [DOI] [PubMed] [Google Scholar]
  9. Odds F. C., EVANS E. G., Holland K. T. Detection of Candida precipitins. a comparison of double diffusion and counter immunoelectrophoresis. J Immunol Methods. 1975 Jun;7(2-3):211–218. doi: 10.1016/0022-1759(75)90018-6. [DOI] [PubMed] [Google Scholar]
  10. Parratt D., Nielsen K. H., Boyd G., White R. G. The quantitation of antibody in farmer's lung syndrome using a radioimmunoassay. Results of a clinical survey and comparison of three serological methods. Clin Exp Immunol. 1975 May;20(2):217–225. [PMC free article] [PubMed] [Google Scholar]
  11. Preisler H. D., Hasenclever H. F., Levitan A. A., Henderson E. S. Serologic diagnosis of disseminated candidiasis in patients with acute leukemia. Ann Intern Med. 1969 Jan;70(1):19–30. doi: 10.7326/0003-4819-70-1-19. [DOI] [PubMed] [Google Scholar]
  12. STALLYBRASS F. C. CANDIDA PRECIPITINS. J Pathol Bacteriol. 1964 Jan;87:89–97. doi: 10.1002/path.1700870113. [DOI] [PubMed] [Google Scholar]
  13. Seelig M. S. The role of antibiotics in the pathogenesis of Candidainfections. Am J Med. 1966 Jun;40(6):887–917. doi: 10.1016/0002-9343(66)90204-x. [DOI] [PubMed] [Google Scholar]
  14. Winner H. I. The transition from commensalism to parasitism. Br J Dermatol. 1969;81(Suppl):62+–62+. doi: 10.1111/j.1365-2133.1969.tb12835.x. [DOI] [PubMed] [Google Scholar]

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