Abstract
A simple automated method for the estimation of ammonia in perchloric acid supernate of blood or plasma using an ion-selective electrode (Orion Ammonia-selective electrode, Model 95-10) is described. The reliability of the proposed method has been checked against an ion-exchange resin procedure, which has been chosen as a standard procedure. Regression equation and correlation coefficient for the proposed method are y = 0.7 X + 10 and 0.945, respectively, as compared with the chosen standard method. Within-run and between-run precision are 2.1% and 3.5% respectively. The average percent recovery is 97.5% and a tentative range is 13-73 microgram/dl (9-52 micronmol/l) ammonia nitrogen.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Conway E. J., Cooke R. Blood ammonia. Biochem J. 1939 Apr;33(4):457–478. doi: 10.1042/bj0330457. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DIENST S. G. An ion exchange method for plasma ammonia concentration. J Lab Clin Med. 1961 Jul;58:149–155. [PubMed] [Google Scholar]
- FORMAN D. T. RAPID DETERMINATION OF PLASMA AMMONIA BY AN ION-EXCHANGE TECHNIC. Clin Chem. 1964 Jun;10:497–508. [PubMed] [Google Scholar]
- Fenton J. C., Williams A. H. Improved method for the estimation of plasma ammonia by ion exchange. J Clin Pathol. 1968 Jan;21(1):14–18. doi: 10.1136/jcp.21.1.14. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HUTCHINSON J. H., LABBY D. H. New method for microdetermination of blood ammonia by use of cation exchange resin. J Lab Clin Med. 1962 Jul;60:170–178. [PubMed] [Google Scholar]
- Jacobs H. A., Olthuis F. M. A kinetic determination of ammonia in plasma. Clin Chim Acta. 1973 Jan 10;43(1):81–86. doi: 10.1016/0009-8981(73)90121-6. [DOI] [PubMed] [Google Scholar]
- Mondzac A., Ehrlich G. E., Seegmiller J. E. An enzymatic determination of ammonia in biological fluids. J Lab Clin Med. 1965 Sep;66(3):526–531. [PubMed] [Google Scholar]
- NATHAN D. G., RODKEY F. L. A colorimetric procedure for the determination of blood ammonia. J Lab Clin Med. 1957 May;49(5):779–785. [PubMed] [Google Scholar]
- Park N. J., Fenton J. C. A simple method for the estimation of plasma ammonia using an ion specific electrode. J Clin Pathol. 1973 Oct;26(10):802–804. doi: 10.1136/jcp.26.10.802. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Proelss H. F., Wright B. W. Rapid determination of ammonia in a perchloric acid supernate from blood, by use of an ammonia-specific electrode. Clin Chem. 1973 Oct;19(10):1162–1169. [PubMed] [Google Scholar]