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. 1979 Jan;32(1):1–10. doi: 10.1136/jcp.32.1.1

Viruses in the stools.

C R Madeley
PMCID: PMC1145558  PMID: 219041

Abstract

It has long been possible to isolate viruses from the stools by culture, though the viruses found are rarely implicated in disease of the gut. In contrast, only recently has it been possible to identify viruses in the stools of patients with diarrhoea. Initially, such identifications were made by electron microscopy but the unsuitability of the microscope for large-scale screening has led to the development of other methods. The new methods have concentrated on rotaviruses but other viruses are also implicated and an overall view of the significance of finding a virus in any stool specimen has to take into account the evidence about all viruses, old and new.

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Selected References

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  1. Agus S. G., Dolin R., Wyatt R. G., Tousimis A. J., Northrup R. S. Acute infectious nonbacterial gastroenteritis: intestinal histopathology. Histologic and enzymatic alterations during illness produced by the Norwalk agent in man. Ann Intern Med. 1973 Jul;79(1):18–25. doi: 10.7326/0003-4819-79-1-18. [DOI] [PubMed] [Google Scholar]
  2. Appleton H., Buckley M., Thom B. T., Cotton J. L., Henderson S. Virus-like particles in winter vomiting disease. Lancet. 1977 Feb 19;1(8008):409–411. doi: 10.1016/s0140-6736(77)92614-9. [DOI] [PubMed] [Google Scholar]
  3. Appleton H., Pereira M. S. A possible virus aetiology in outbreaks of food-poisoning from cockles. Lancet. 1977 Apr 9;1(8015):780–781. doi: 10.1016/s0140-6736(77)92960-9. [DOI] [PubMed] [Google Scholar]
  4. Aronson M. D., Phillips C. A., Beeken W. L., Forsyth B. R. Isolation and characterization of a viral agent from intestinal tissue of patients with Crohn's disease and other intestinal disorders. Prog Med Virol. 1975;21:165–176. [PubMed] [Google Scholar]
  5. Banatvala J. E., Totterdell B., Chrystie I. L., Woode G. N. In-vitro detection of human rotaviruses. Lancet. 1975 Oct 25;2(7939):821–821. doi: 10.1016/s0140-6736(75)80057-2. [DOI] [PubMed] [Google Scholar]
  6. Bishop R. F., Davidson G. P., Holmes I. H., Ruck B. J. Virus particles in epithelial cells of duodenal mucosa from children with acute non-bacterial gastroenteritis. Lancet. 1973 Dec 8;2(7841):1281–1283. doi: 10.1016/s0140-6736(73)92867-5. [DOI] [PubMed] [Google Scholar]
  7. Bryden A. S., Davies H. A., Thouless M. E., Flewitt T. H. Diagnosis of rotavirus infection by cell culture. J Med Microbiol. 1977 Feb;10(1):121–125. doi: 10.1099/00222615-10-1-121. [DOI] [PubMed] [Google Scholar]
  8. Caul E. O., Clarke S. K. Coronavirus propagated from patient with non-bacterial gastroenteritis. Lancet. 1975 Nov 15;2(7942):953–954. doi: 10.1016/S0140-6736(75)90363-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Cave D. R., Mitchell D. N., Kane S. P., Brooke B. N. Further animal evidence of a transmissible agent in Crohn's disease. Lancet. 1973 Nov 17;2(7838):1120–1122. doi: 10.1016/s0140-6736(73)90936-7. [DOI] [PubMed] [Google Scholar]
  10. Chrystie I. L., Totterdell B., Baker M. J., Scopes J. W., Banatvala J. E. Letter: Rotavirus infections in a maternity unit. Lancet. 1975 Jul 12;2(7924):79–79. doi: 10.1016/s0140-6736(75)90525-5. [DOI] [PubMed] [Google Scholar]
  11. Clarke S. K., Cook G. T., Egglestone S. I., Hall T. S., Miller D. L., Reed S. E., Rubenstein D., Smith A. J., Tyrrell D. A. A virus from epidemic vomiting disease. Br Med J. 1972 Jul 8;3(5818):86–89. doi: 10.1136/bmj.3.5818.86. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Davidson G. P., Bishop R. F., Townley R. R., Holmes I. H. Importance of a new virus in acute sporadic enteritis in children. Lancet. 1975 Feb 1;1(7901):242–246. doi: 10.1016/s0140-6736(75)91140-x. [DOI] [PubMed] [Google Scholar]
  13. EICHENWALD H. F., ABABIO A., ARKY A. M., HARTMAN A. P. Epidemic diarrhea in premature and older infants caused by ECHO virus type 18. J Am Med Assoc. 1958 Mar 29;166(13):1563–1566. doi: 10.1001/jama.1958.02990130023005. [DOI] [PubMed] [Google Scholar]
  14. Elias M. M. Distribution and titres of rotavirus antibodies in different age groups. J Hyg (Lond) 1977 Dec;79(3):365–372. doi: 10.1017/s0022172400053201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Feinstone S. M., Kapikian A. Z., Purceli R. H. Hepatitis A: detection by immune electron microscopy of a viruslike antigen associated with acute illness. Science. 1973 Dec 7;182(4116):1026–1028. doi: 10.1126/science.182.4116.1026. [DOI] [PubMed] [Google Scholar]
  16. Fox J. P., Brandt C. D., Wassermann F. E., Hall C. E., Spigland I., Kogon A., Elveback L. R. The virus watch program: a continuing surveillance of viral infections in metropolitan New York families. VI. Observations of adenovirus infections: virus excretion patterns, antibody response, efficiency of surveillance, patterns of infections, and relation to illness. Am J Epidemiol. 1969 Jan;89(1):25–50. doi: 10.1093/oxfordjournals.aje.a120913. [DOI] [PubMed] [Google Scholar]
  17. Gitnick G. L., Arthur M. H., Shibata I. Cultivation of viral agents from Crohn's disease. A new sensitive system. Lancet. 1976 Jul 31;2(7979):215–217. doi: 10.1016/s0140-6736(76)91022-9. [DOI] [PubMed] [Google Scholar]
  18. Holmes I. H., Rodger S. M., Schnagl R. D., Ruck B. J., Gust I. D., Bishop R. F., Barnes G. L. Is lactase the receptor and uncoating enzyme for infantile enteritis (rota) viruses? Lancet. 1976 Jun 26;1(7974):1387–1388. doi: 10.1016/s0140-6736(76)93032-4. [DOI] [PubMed] [Google Scholar]
  19. Kalica A. R., Purcell R. H., Sereno M. M., Wyatt R. G., Kim H. W., Chanock R. M., Kapikian A. Z. A microtiter solid phase radioimmunoassay for detection of the human reovirus-like agent in stools. J Immunol. 1977 Apr;118(4):1275–1279. [PubMed] [Google Scholar]
  20. Kapikian A. Z., Cline W. L., Mebus C. A., Wyatt R. G., Kalica A. R., James H. D., Jr, VanKirk D., Chanock R. M. New complement-fixation test for the human reovirus-like agent of infantile gastroenteritis. Nebraska calf diarrhea virus used as antigen. Lancet. 1975 May 10;1(7915):1056–1061. doi: 10.1016/s0140-6736(75)91827-9. [DOI] [PubMed] [Google Scholar]
  21. Kapikian A. Z., Wyatt R. G., Dolin R., Thornhill T. S., Kalica A. R., Chanock R. M. Visualization by immune electron microscopy of a 27-nm particle associated with acute infectious nonbacterial gastroenteritis. J Virol. 1972 Nov;10(5):1075–1081. doi: 10.1128/jvi.10.5.1075-1081.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Kurtz J. B., Lee T. W., Pickering D. Astrovirus associated gastroenteritis in a children's ward. J Clin Pathol. 1977 Oct;30(10):948–952. doi: 10.1136/jcp.30.10.948. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Madeley C. R., Cosgrove B. P., Bell E. J., Fallon R. J. Stool viruses in babies in Glasgow. I. Hospital admissions with diarrhoea. J Hyg (Lond) 1977 Apr;78(2):261–273. doi: 10.1017/s0022172400056151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Madeley C. R., Cosgrove B. P., Bell E. J. Stool viruses in babies in Glasgow 2. Investigation of normal newborns in hospital. J Hyg (Lond) 1978 Oct;81(2):285–294. doi: 10.1017/s0022172400025122. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Matthews T. H., Nair C. D., Lawrence M. K., Tyrrell D. A. Antiviral activity in milk of possible clinical importance. Lancet. 1976 Dec 25;2(8000):1387–1389. doi: 10.1016/s0140-6736(76)91922-x. [DOI] [PubMed] [Google Scholar]
  26. Mebus C. A., Wyatt R. G., Kapikian A. Z. Intestinal lesions induced in gnotobiotic calves by the virus of human infantile gastroenteritis. Vet Pathol. 1977 May;14(3):273–282. doi: 10.1177/030098587701400310. [DOI] [PubMed] [Google Scholar]
  27. Meurman O. H., Laine M. J. Rotavirus epidemic in adults. N Engl J Med. 1977 Jun 2;296(22):1298–1299. doi: 10.1056/NEJM197706022962220. [DOI] [PubMed] [Google Scholar]
  28. Middleton P. J., Petric M., Hewitt C. M., Szymanski M. T., Tam J. S. Counter-immunoelectro-osmophoresis for the detection of infantile gastroenteritis virus (orbi-group) antigen and antibody. J Clin Pathol. 1976 Mar;29(3):191–197. doi: 10.1136/jcp.29.3.191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Middleton P. J., Petric M., Szymanski M. T. Propagation of infantile gastroenteritis virus (orbi-group) in conventional and germfree piglets. Infect Immun. 1975 Dec;12(6):1276–1280. doi: 10.1128/iai.12.6.1276-1280.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Middleton P. J., Szymanski M. T., Petric M. Viruses associated with acute gastroenteritis in young children. Am J Dis Child. 1977 Jul;131(7):733–737. doi: 10.1001/archpedi.1977.02120200015004. [DOI] [PubMed] [Google Scholar]
  31. Morris C. A., Flewett T. H., Bryden A. S., Davies H. Epidemic viral enteritis in a long-stay children's ward. Lancet. 1975 Jan 4;1(7897):4–5. doi: 10.1016/s0140-6736(75)92370-3. [DOI] [PubMed] [Google Scholar]
  32. Murphy A. M., Albrey M. B., Crewe E. B. Rotavirus infections of neonates. Lancet. 1977 Dec 3;2(8049):1149–1150. doi: 10.1016/S0140-6736(77)91538-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Orstavik I., Haug K. W., Sovde A. Rotavirus-associated gastroenteritis in two adults probably caused by virus reinfection. Scand J Infect Dis. 1976;8(4):277–278. doi: 10.3109/inf.1976.8.issue-4.12. [DOI] [PubMed] [Google Scholar]
  34. PATTERSON W. J., BELL E. J. Poliomyelitis in a nursery school in Glasgow. Br Med J. 1963 Jun 15;1(5345):1574–1576. doi: 10.1136/bmj.1.5345.1574. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Pertschuk L. P., Cook A. W., Gupta J. K., Broome J. D., Vuletin J. C., Kim D. S., Brigati D. J., Rainford E. A., Nidsgorski F. Jejunal immunopathology in amyotrophic lateral sclerosis and multiple sclerosis. Identification of viral antigens by immunofluorescence. Lancet. 1977 May 28;1(8022):1119–1125. doi: 10.1016/s0140-6736(77)92382-0. [DOI] [PubMed] [Google Scholar]
  36. Prasad I., Broome J. D., Pertschuk L. P., Gupta J., Cook A. W. Recovery of Paramyxovirus from the jejunum of patients with multiple sclerosis. Lancet. 1977 May 28;1(8022):1117–1119. doi: 10.1016/s0140-6736(77)92381-9. [DOI] [PubMed] [Google Scholar]
  37. Schoub B. D., Koornhof H. J., Lecatsas G., Prozesky O. W., Freiman I., Hartman E., Kassel H. Letter: Viruses in acute summer gastroenteritis in black infants. Lancet. 1975 May 10;1(7915):1093–1094. doi: 10.1016/s0140-6736(75)91869-3. [DOI] [PubMed] [Google Scholar]
  38. Simhon A., Mata L. Anti-rotavirus antibody in human colostrum. Lancet. 1978 Jan 7;1(8054):39–40. doi: 10.1016/s0140-6736(78)90382-3. [DOI] [PubMed] [Google Scholar]
  39. Snodgrass D. R., Angus K. W., Gray E. W. Rotavirus infection in lambs: pathogenesis and pathology. Arch Virol. 1977;55(4):263–274. doi: 10.1007/BF01315048. [DOI] [PubMed] [Google Scholar]
  40. Snodgrass D. R., Madeley C. R., Wells P. W., Angus K. W. Human rotavirus in lambs: infection and passive protection. Infect Immun. 1977 Apr;16(1):268–270. doi: 10.1128/iai.16.1.268-270.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Snodgrass D. R., Wells P. W. Rotavirus infection in lambs: studies on passive protection. Arch Virol. 1976;52(3):201–205. doi: 10.1007/BF01348017. [DOI] [PubMed] [Google Scholar]
  42. Spence L., Fauvel M., Petro R., Bloch S. Haemagglutinin from Rotavirus. Lancet. 1976 Nov 6;2(7993):1023–1023. doi: 10.1016/s0140-6736(76)90860-6. [DOI] [PubMed] [Google Scholar]
  43. Thouless M. E., Bryden A. S., Flewett T. H. Rotavirus neutralisation by human milk. Br Med J. 1977 Nov 26;2(6099):1390–1390. doi: 10.1136/bmj.2.6099.1390. [DOI] [PMC free article] [PubMed] [Google Scholar]
  44. Thouless M. E., Bryden A. S., Flewett T. H. Serotypes of human rotavirus. Lancet. 1978 Jan 7;1(8054):39–39. doi: 10.1016/s0140-6736(78)90381-1. [DOI] [PubMed] [Google Scholar]
  45. Totterdell B. M., Chrystie I. L., Banatvala J. E. Rotavirus infections in a maternity unit. Arch Dis Child. 1976 Dec;51(12):924–928. doi: 10.1136/adc.51.12.924. [DOI] [PMC free article] [PubMed] [Google Scholar]
  46. Tufvesson B., Johnsson T. Occurrence of reo-like calf viruses in young children with acute gastroenteritis. Diagnoses established by electron microscopy and complement fixation, using the reo-like virus as antigen. Acta Pathol Microbiol Scand B. 1976 Feb;84(1):22–28. [PubMed] [Google Scholar]
  47. Watanabe H., Holmes I. H. Filter paper solid-phase radioimmunoassay for human rotavirus surface immunoglobulins. J Clin Microbiol. 1977 Oct;6(4):319–324. doi: 10.1128/jcm.6.4.319-324.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  48. White G. B., Ashton C. I., Roberts C., Parry H. E. Letter: "Rotavirus" in gastroenteritis. Lancet. 1974 Sep 21;2(7882):726–726. doi: 10.1016/s0140-6736(74)93308-x. [DOI] [PubMed] [Google Scholar]
  49. White G. B., Stancliffe D. Letter: Viruses and gastroenteritis. Lancet. 1975 Oct 11;2(7937):703–703. [PubMed] [Google Scholar]
  50. Whitelaw A., Davies H., Parry J. Eelectron microscopy of fatal adenovirus gastroenteritis. Lancet. 1977 Feb 12;1(8007):361–361. doi: 10.1016/s0140-6736(77)91158-8. [DOI] [PubMed] [Google Scholar]
  51. Whorwell P. J., Phillips C. A., Beeken W. L., Little P. K., Roessner K. D. Isolation of reovirus-like agents from patients with Crohn's disease. Lancet. 1977 Jun 4;1(8023):1169–1171. doi: 10.1016/s0140-6736(77)92714-3. [DOI] [PubMed] [Google Scholar]
  52. Wyatt R. G., Gill V. W., Sereno M. M., Kalica A. R., VanKirk D. H., Chanock R. M., Kapikian A. Z. Letter: Probable in-vitro cultivation of human reovirus-like agent of infantile diarroea. Lancet. 1976 Jan 10;1(7950):98–99. doi: 10.1016/s0140-6736(76)90202-6. [DOI] [PubMed] [Google Scholar]
  53. Yolken R. H., Kim H. W., Clem T., Wyatt R. G., Kalica A. R., Chanock R. M., Kapikian A. Z. Enzyme-linked immunosorbent assay (ELISA) for detection of human reovirus-like agent of infantile gastroenteritis. Lancet. 1977 Aug 6;2(8032):263–267. doi: 10.1016/s0140-6736(77)90951-5. [DOI] [PubMed] [Google Scholar]
  54. Yolken R. H., Wyatt R. G., Kalica A. R., Kim H. W., Brandt C. D., Parrott R. H., Kapikian A. Z., Chanock R. M. Use of a free viral immunofluorescence assay to detect human reovirus-like agent in human stools. Infect Immun. 1977 May;16(2):467–470. doi: 10.1128/iai.16.2.467-470.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  55. Zissis G., Lambert J. P., De Kegel D. Routine diagnosis of human rotaviruses in stools. J Clin Pathol. 1978 Feb;31(2):175–178. doi: 10.1136/jcp.31.2.175. [DOI] [PMC free article] [PubMed] [Google Scholar]
  56. Zissis G., Lambert J. P. Different serotypes of human rotaviruses. Lancet. 1978 Jan 7;1(8054):38–39. doi: 10.1016/s0140-6736(78)90380-x. [DOI] [PubMed] [Google Scholar]
  57. von Bonsdorff C. H., Hovi T., Mákelá P., Hovi T., Tevalvoto-Aarnio M. Rotavirus associated with acute gastroenteritis in adults. Lancet. 1976 Aug 21;2(7982):423–423. doi: 10.1016/s0140-6736(76)92445-4. [DOI] [PubMed] [Google Scholar]
  58. von Bonsdorff C. H., Hovi T., Mäkelä P., Mörttinen A. Rotavirus infections in adults in association with acute gastroenteritis. J Med Virol. 1978;2(1):21–28. doi: 10.1002/jmv.1890020105. [DOI] [PubMed] [Google Scholar]

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