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Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1979 Jan;32(1):35–39. doi: 10.1136/jcp.32.1.35

A new antigen on the epithelial membrane: its immunoperoxidase localisation in normal and neoplastic tissue.

E Heyderman, K Steele, M G Ormerod
PMCID: PMC1145564  PMID: 372248

Abstract

Using antisera raised against defatted human cream we have demonstrated a new antigen on the epithelial membrane. An indirect immunoperoxidase technique on routinely processed histological sections was used, and we describe results which show that this antigen has a widespread but highly selective distribution, being apparently localised to membranes that have a secretory function. The antigen is carried by a variety of adenocarcinomas, suggesting that its localisation may have a role in the diagnosis and differential diagnosis of malignant disease.

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Selected References

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  1. Ceriani R. L., Thompson K., Peterson J. A., Abraham S. Surface differentiation antigens of human mammary epithelial cells carried on the human milk fat globule. Proc Natl Acad Sci U S A. 1977 Feb;74(2):582–586. doi: 10.1073/pnas.74.2.582. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Edgington T. S., Glassock R. J., Watson J. I., Dixon F. J. Characterization and isolation of specific renal tubular epithelial antigens. J Immunol. 1967 Dec;99(6):1199–1210. [PubMed] [Google Scholar]
  3. Friedman T. Immunofluorescent localization of Tamm-Horsfall mucoprotein. Experientia. 1966 Sep 15;22(9):624–625. doi: 10.1007/BF01895292. [DOI] [PubMed] [Google Scholar]
  4. Fritsché R., Mach J. Identification of a new oncofoetal antigen associated with several types of human carcinomas. Nature. 1975 Dec 25;258(5537):734–737. doi: 10.1038/258734a0. [DOI] [PubMed] [Google Scholar]
  5. Haagensen D. E., Jr, Mazoujian G., Holder W. D., Jr, Kister S. J., Wells S. A., Jr Evaluation of a breast cyst fluid protein detectable in the plasma of breast carcinoma patients. Ann Surg. 1977 Mar;185(3):279–285. doi: 10.1097/00000658-197703000-00005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Heyderman E., Neville A. M. A shorter immunoperoxidase technique for the demonstration of carcinoembryonic antigen and other cell products. J Clin Pathol. 1977 Feb;30(2):138–140. doi: 10.1136/jcp.30.2.138. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Nakane P. K., Kawaoi A. Peroxidase-labeled antibody. A new method of conjugation. J Histochem Cytochem. 1974 Dec;22(12):1084–1091. doi: 10.1177/22.12.1084. [DOI] [PubMed] [Google Scholar]
  8. Pascal R. R., Mesa-Tejada R., Bennett S. J., Garces A., Fenoglio C. M. Carcinoembryonic antigen: immunohistologic identification in invasive and intraepithelial carcinomas of the lung. Arch Pathol Lab Med. 1977 Nov;101(11):568–571. [PubMed] [Google Scholar]
  9. Soule H. D., Vazguez J., Long A., Albert S., Brennan M. A human cell line from a pleural effusion derived from a breast carcinoma. J Natl Cancer Inst. 1973 Nov;51(5):1409–1416. doi: 10.1093/jnci/51.5.1409. [DOI] [PubMed] [Google Scholar]
  10. von Kleist S., Chavanel G., Burtin P. Identification of an antigen from normal human tissue that crossreacts with the carcinoembryonic antigen. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2492–2494. doi: 10.1073/pnas.69.9.2492. [DOI] [PMC free article] [PubMed] [Google Scholar]

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