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. 1979 Feb;32(2):122–127. doi: 10.1136/jcp.32.2.122

Comparison of enzyme-linked immunosorbent assay (ELISA) technique and complement-fixation test for estimation of cytomegalovirus IgG antibody.

J C Booth, G Hannington, T A Aziz, H Stern
PMCID: PMC1145595  PMID: 220285

Abstract

The ELISA technique has been found to be reliable for the detection and titration of cytomegalovirus-specific IgG antibody in serum. It is about six times more sensitive than the CF test although some discrepancies were found between the antibody titres determined by the two methods.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bernstein M. T., Stewart J. A. Indirect hemagglutination test for detection of antibodies to Cytomegalovirus. Appl Microbiol. 1971 Jan;21(1):84–89. doi: 10.1128/am.21.1.84-89.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Betts R. F., George S. D., Rundell R. B., Freeman R. B., Douglas R. G., Jr Comparative activity of immunofluorescent antibody and complement-fixing antibody in cytomegalovirus infection. J Clin Microbiol. 1976 Aug;4(2):151–156. doi: 10.1128/jcm.4.2.151-156.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Castellano G. A., Hazzard G. T., Madden D. L., Sever J. L. Comparison of the enzyme-linked immunosorbent assay and the indirect hemagglutination test for detection of antibody to cytomegalovirus. J Infect Dis. 1977 Oct;136 (Suppl):S337–S340. doi: 10.1093/infdis/136.supplement_2.s337. [DOI] [PubMed] [Google Scholar]
  4. Cremer N. E., Schmidt N. J., Jensen F., Hoffman M., Oshiro L. S., Lennette E. H. Complement-fixing antibody in human sera reactive with viral and soluble antigens of cytomegalovirus. J Clin Microbiol. 1975 Mar;1(3):262–267. doi: 10.1128/jcm.1.3.262-267.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dienstag J. L., Cline W. L., Purcell R. H. Detection of cytomegalovirus antibody by immune adherence hemagglutination. Proc Soc Exp Biol Med. 1976 Dec;153(3):543–548. doi: 10.3181/00379727-153-39588. [DOI] [PubMed] [Google Scholar]
  6. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol. 1972 Jul;109(1):129–135. [PubMed] [Google Scholar]
  7. Forghani B., Schmidt N. J., Lennette E. H. Antisera to human cytomegalovirus produced in hamsters: reactivity in radioimmunoassay and other antibody assay systems. Infect Immun. 1976 Nov;14(5):1184–1190. doi: 10.1128/iai.14.5.1184-1190.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fuccillo D. A., Moder F., Traub R. G., Hensen S., Sever J. L. Micro indirect hemagglutination test for Cytomegalovirus. Appl Microbiol. 1971 Jan;21(1):104–107. doi: 10.1128/am.21.1.104-107.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Griffiths P. D., Buie K. J., Heath R. B. A comparison of complement fixation, indirect immunofluorescence for viral late antigens, and anti-complement immunofluorescence tests for the detection of cytomegalovirus specific serum antibodies. J Clin Pathol. 1978 Sep;31(9):827–831. doi: 10.1136/jcp.31.9.827. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hanshaw J. B., Steinfeld H. J., White C. J. Fluorescent-antibody test for cytomegalovirus macroglobulin. N Engl J Med. 1968 Sep 12;279(11):566–570. doi: 10.1056/NEJM196809122791102. [DOI] [PubMed] [Google Scholar]
  11. Haukenes G. Negative complement-fixation tests with rheumatoid factor positive sera. A simple method for selective removal of rheumatoid factor. Ann Rheum Dis. 1974 Sep;33(5):461–464. doi: 10.1136/ard.33.5.461. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jacobs J. P., Jones C. M., Baille J. P. Characteristics of a human diploid cell designated MRC-5. Nature. 1970 Jul 11;227(5254):168–170. doi: 10.1038/227168a0. [DOI] [PubMed] [Google Scholar]
  13. Kettering J. D., Schmidt N. J., Lennette E. H. Improved glycine-extracted complement-fixing antigen for human cytomegalovirus. J Clin Microbiol. 1977 Dec;6(6):647–649. doi: 10.1128/jcm.6.6.647-649.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Knez V., Stewart J. A., Ziegler D. W. Cytomegalovirus specific IgM and IgG response in humans studied by radioimmunoassay. J Immunol. 1976 Nov;117(5 PT2):2006–2013. [PubMed] [Google Scholar]
  15. Krech U., Jung M., Sonnabend W. A study of complement-fixing, immunofluorescent, and neutralizing antibodies in human cytomegalovirus infections. Z Immunitatsforsch Exp Klin Immunol. 1971 Jun;141(5):411–429. [PubMed] [Google Scholar]
  16. SEVER J. L. Application of a microtechnique to viral serological investigations. J Immunol. 1962 Mar;88:320–329. [PubMed] [Google Scholar]
  17. Sakuma S., Furukawa T., Plotkin S. A. The characterization of IgG receptor induced by human cytomegalovirus. Proc Soc Exp Biol Med. 1977 Jun;155(2):168–172. doi: 10.3181/00379727-155-39767. [DOI] [PubMed] [Google Scholar]
  18. Schmitz H., Doerr H. W., Kampa D., Vogt A. Solid-phase enzyme immunoassay for immunoglobulin M antibodies to cytomegalovirus. J Clin Microbiol. 1977 Jun;5(6):629–634. doi: 10.1128/jcm.5.6.629-634.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Stanford F. Comparison of complement-fixing antibody titres in patients with rheumatoid arthritis and matched controls. Ann Rheum Dis. 1972 Jul;31(4):330–333. doi: 10.1136/ard.31.4.330. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. The T. H., Klein G., Langenhuysen M. M. Antibody reactions to virus-specific early antigens (EA) in patients with cytomegalovirus (CMV) infection. Clin Exp Immunol. 1974 Jan;16(1):1-7,9-12. [PMC free article] [PubMed] [Google Scholar]
  21. Voller A., Bidwell D. E. Enzyme-immunoassays for antibodies in measles, cytomegalovirus infections and after rubella vaccination. Br J Exp Pathol. 1976 Apr;57(2):243–247. [PMC free article] [PubMed] [Google Scholar]

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