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Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1979 Feb;32(2):136–142. doi: 10.1136/jcp.32.2.136

Rapid serotyping of groups A, B, and C meningococci by rocket-line immunoelectrophoresis and co-agglutination.

D Danielsson, P Olcén
PMCID: PMC1145598  PMID: 108320

Abstract

Rocket-line immunoelectrophoresis (R-LIE) with antigen containing intermediate gel, and co-agglutination utilising protein A-containing staphylococci coated with specific antibodies, were adapted for serotyping the prototypes of group B meningococci. Both were found to have the same specificity as agar gel double diffusion (AGDD) but they were more sensitive and more rapid than AGDD. R-LIE required, like AGDD, the extraction of relatively large quantities of bacteria, while the co-agglutination method, performed as a slide agglutination tests with results within a few minutes and no need of special equipment, required only a small amount of heated whole meningococcal cells. Meningococcal strains of serogroups B, C, and A from patients with carriers were serotyped and the results with all three methods were in agreement.

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Selected References

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