Skip to main content
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1979 Sep;32(9):902–906. doi: 10.1136/jcp.32.9.902

Serum gamma-glutamyltransferase isoenzymes in extrahepatic biliary obstruction.

P R Wenham, C P Price, H G Sammons
PMCID: PMC1145849  PMID: 41851

Abstract

The gamma-glutamyltransferase isoenzymes in the sera of patients with extrahepatic biliary obstruction have been studied, using electrophoretic, gel filtration, and ultracentrifugation techniques, and compared with those present in normal sera. Five isoenzymes were shown to exist in patients' sera, three of which were not demonstrated in normal sera. The observations are discussed in relation to the influence of biliary regurgitation and the possible solubilisation of membrane-bound enzymes. The results are compared with those of previous studies on alkaline phosphatase.

Full text

PDF
902

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Azzopardi O., Jayle M. F. Formes moléculaires multiples de la gamma-glutamyl-transpeptidase. Clin Chim Acta. 1973 Jan 24;43(2):163–169. doi: 10.1016/0009-8981(73)90447-6. [DOI] [PubMed] [Google Scholar]
  2. Beck P. R. Butanol extraction of serum and urinary gamma-glutamyltransferase and its application in clinical diagnosis. Ann Clin Biochem. 1978 May;15(3):151–156. doi: 10.1177/000456327801500131. [DOI] [PubMed] [Google Scholar]
  3. De Broe M. E., Borgers M., Wieme R. J. The separation and characterization of liver plasma membrane fragments circulating in the blood of patients with cholestasis. Clin Chim Acta. 1975 Mar 24;59(3):369–372. doi: 10.1016/0009-8981(75)90013-3. [DOI] [PubMed] [Google Scholar]
  4. De Broe M. E., Wieme R. J., Logghe G. N., Roels F. Spontaneous shedding of plasma membrane fragments by human cells in vivo and in vitro. Clin Chim Acta. 1977 Dec 15;81(3):237–245. doi: 10.1016/0009-8981(77)90054-7. [DOI] [PubMed] [Google Scholar]
  5. Dunne J., Fennelly J. J., McGeeney K. Separation of alkaline phosphatase enzymes in human serum using gel-filtration (Sephadex G-200) techniques. Cancer. 1967 Jan;20(1):71–76. doi: 10.1002/1097-0142(1967)20:1<71::aid-cncr2820200111>3.0.co;2-v. [DOI] [PubMed] [Google Scholar]
  6. FLODIN P., KILLANDER J. Fractionation of human-serum proteins by gel filtration. Biochim Biophys Acta. 1962 Oct 8;63:402–410. doi: 10.1016/0006-3002(62)90104-x. [DOI] [PubMed] [Google Scholar]
  7. Hetland O., Andersson T. R., Gerner T. The heterogeneity of the serum activity of gamma-glutamyl transpeptidase in hepatobiliary diseases as studied by agarose gel electrophoresis. Clin Chim Acta. 1975 Aug 4;62(3):425–431. doi: 10.1016/0009-8981(75)90094-7. [DOI] [PubMed] [Google Scholar]
  8. Huseby N. E. Multiple forms of gamma-glutamyltransferase in normal human liver, bile and serum. Biochim Biophys Acta. 1978 Feb 10;522(2):354–362. doi: 10.1016/0005-2744(78)90069-4. [DOI] [PubMed] [Google Scholar]
  9. Huseby N. E., Vik T. The activity of gamma-glutamyltransferase after bile duct ligation in guinea pig. Clin Chim Acta. 1978 Sep 1;88(2):385–392. doi: 10.1016/0009-8981(78)90445-x. [DOI] [PubMed] [Google Scholar]
  10. Jennings R. C., Brocklehurst D., Hirst M. A comparative study of alkaline phosphatase enzymes using starch-gel electrophoresis and sephadex gel-filtration with special reference to high molecular weight enzymes. Clin Chim Acta. 1970 Nov;30(2):509–517. doi: 10.1016/0009-8981(70)90144-0. [DOI] [PubMed] [Google Scholar]
  11. Kokot F., Kuska J. Heterogeneity of serum gamma-glutamyl transpeptidase in different internal diseases studied by starch gel electrophoresis and Sephadex filtration. Enzymol Biol Clin (Basel) 1968;9(1):59–67. doi: 10.1159/000458235. [DOI] [PubMed] [Google Scholar]
  12. Kryszewski A. J., Neale G., Whitfield J. B., Moss D. W. Enzyme changes in experimental biliary obstruction. Clin Chim Acta. 1973 Aug 30;47(2):175–182. doi: 10.1016/0009-8981(73)90313-6. [DOI] [PubMed] [Google Scholar]
  13. MOSS D. W. Iso-enzymes of alkaline phosphatase in autolysed and butanol-extracted liver preparations. Nature. 1962 Mar 10;193:981–982. doi: 10.1038/193981a0. [DOI] [PubMed] [Google Scholar]
  14. Miyazaki S., Okumura M. Change of serum -glutamyl transpeptidase level and isoenzyme pattern in hepatobiliary pancreatic disease. Clin Chim Acta. 1972 Aug;40(1):193–197. doi: 10.1016/0009-8981(72)90270-7. [DOI] [PubMed] [Google Scholar]
  15. ORLOWSKI M. THE ROLE OF GAMMA-GLUTAMYL TRANSPEPTIDASE IN THE INTERNAL DISEASES CLINIC. Arch Immunol Ther Exp (Warsz) 1963;11:1–61. [PubMed] [Google Scholar]
  16. Orlowski M., Szczeklik A. Heterogeneity of serum gamma-glutamyl transpeptidase in hepatobiliary diseases. Clin Chim Acta. 1967 Mar;15(3):387–391. doi: 10.1016/0009-8981(67)90002-2. [DOI] [PubMed] [Google Scholar]
  17. Price C. P., Hill P. G., Sammons H. G. The nature of the alkaline phosphatases of bile. J Clin Pathol. 1972 Feb;25(2):149–154. doi: 10.1136/jcp.25.2.149. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Price C. P., Sammons H. G. The nature of the serum alkaline phosphatases in liver diseases. J Clin Pathol. 1974 May;27(5):392–398. doi: 10.1136/jcp.27.5.392. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. RUTTENBURG A. M., GOLDBARG J. A., PINEDA E. P. SERUM GAMMA-GLUTAMYL TRANSPEPTIDASE ACTIVITY IN HEPATOBILIARY PANCREATIC DISEASE. Gastroenterology. 1963 Jul;45:43–48. [PubMed] [Google Scholar]
  20. Rosalki S. B., Tarlow D. Optimized determination of gamma-glutamyltransferase by reaction-rate analysis. Clin Chem. 1974 Sep;20(9):1121–1124. [PubMed] [Google Scholar]
  21. Szewczuk A. A soluble form of gamma-glutamyl transpeptidase in human tissues. Clin Chim Acta. 1966 Nov;14(5):608–614. doi: 10.1016/0009-8981(66)90184-7. [DOI] [PubMed] [Google Scholar]
  22. Wenham P. R., Price C. P., Sammons H. G. A short review of techniques for the localisation of gamma-glutamyl transferase isoenzymes after electrophoresis. Ann Clin Biochem. 1978 May;15(3):146–150. doi: 10.1177/000456327801500130. [DOI] [PubMed] [Google Scholar]
  23. Wenham P. R., Price C. P., Sammons H. G. gamma-Glutamyl transferase isoenzymes in human bile. J Clin Pathol. 1978 Jul;31(7):666–670. doi: 10.1136/jcp.31.7.666. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Whitfield J. B., Pounder R. E., Neale G., Moss D. W. Serum -glytamyl transpeptidase activity in liver disease. Gut. 1972 Sep;13(9):702–708. doi: 10.1136/gut.13.9.702. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES