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. 1979 Dec;32(12):1272–1277. doi: 10.1136/jcp.32.12.1272

A histochemical comparison of the O-acylated sialic acids of the epithelial mucins in ulcerative colitis, Crohn's disease, and normal controls.

C F Culling, P E Reid, W L Dunn
PMCID: PMC1145949  PMID: 94060

Abstract

Two histochemical techniques, the PAT/KOH/PAS and the PBT/KOH/PAS, were used to investigate the side chain O-acyl substitution patterns of the sialic acids of the colonic epithelial mucins in cases of ulcerative colitis and Crohn's disease. In both diseases there was, as compared to normal, a reduction in the proportion of sialic acids O-acylated at C7C8, the reduction being greater in ulcerative colitis. Further, there appeared to be an association between the severity of the disease and the reduction in the staining of O-acylated sialic acids. This relationship was more marked in ulcerative colitis. In some cases of both diseases there was evidence for epithelial mucins containing predominantly C7-substituted sialic acids. This study has confirmed our previous conclusion that, in Crohn's disease of the terminal ileum, the disease is associated with an increase in the proportion of sialic acids bearing side chain substituents.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Culling C. F., Reid P. E., Burton J. D., Dunn W. L. A histochemical method of differentiating lower gastrointestinal tract mucin from other mucins in primary or metastatic tumours. J Clin Pathol. 1975 Aug;28(8):656–658. doi: 10.1136/jcp.28.8.656. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Culling C. F., Reid P. E., Clay M. G., Dunn W. L. The histochemical demonstration of O-acylated sialic acid in gastrointestinal mucins. Their association with the potassium hydroxide-periodic acid-schiff effect. J Histochem Cytochem. 1974 Aug;22(8):826–831. doi: 10.1177/22.8.826. [DOI] [PubMed] [Google Scholar]
  3. Culling C. F., Reid P. E., Dunn W. L. A new histochemical method for the identification and visualization of both side chain acylated and nonacylated sialic acids. J Histochem Cytochem. 1976 Dec;24(12):1225–1230. doi: 10.1177/24.12.187689. [DOI] [PubMed] [Google Scholar]
  4. Culling C. F., Reid P. E., Dunn W. L. A problem in the removal of sialic acid by acid hydrolysis. Stain Technol. 1974 Sep;49(5):317–318. doi: 10.3109/10520297409117003. [DOI] [PubMed] [Google Scholar]
  5. Culling C. F., Reid P. E., Dunn W. L., Clay M. G. Histochemical comparison of the epithelial mucins in the ileum in Crohn's disease and in normal controls. J Clin Pathol. 1977 Nov;30(11):1063–1067. doi: 10.1136/jcp.30.11.1063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Culling C. F., Reid P. E., Dunn W. L. The effect of saponification upon certain histochemical reactions of the epithelial mucins of the gastrointestinal tract. J Histochem Cytochem. 1971 Nov;19(11):654–662. doi: 10.1177/19.11.654. [DOI] [PubMed] [Google Scholar]
  7. Culling C. F., Reid P. E., Worth A. J., Dunn W. L. A new histochemical technique of use in the interpretation and diagnosis of adenocarcinoma and villous lesions in the large intestine. J Clin Pathol. 1977 Nov;30(11):1056–1062. doi: 10.1136/jcp.30.11.1056. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fenger C., Filipe M. I. Pathology of the anal glands with special reference to their mucin histochemistry. Acta Pathol Microbiol Scand A. 1977 May;85(3):273–285. doi: 10.1111/j.1699-0463.1977.tb00428.x. [DOI] [PubMed] [Google Scholar]
  9. Filipe M. I. 35 sulphur uptake in the mucosa adjacent to carcinoma of the large intestine. Histochem J. 1971 Jan;3(1):27–35. doi: 10.1007/BF01686504. [DOI] [PubMed] [Google Scholar]
  10. Filipe M. I., Branfoot A. C. Abnormal patterns of mucus secretion in apparently normal mucosa of large intestine with carcinoma. Cancer. 1974 Aug;34(2):282–290. doi: 10.1002/1097-0142(197408)34:2<282::aid-cncr2820340211>3.0.co;2-w. [DOI] [PubMed] [Google Scholar]
  11. Filipe M. I., Branfoot A. C. Mucin histochemistry of the colon. Curr Top Pathol. 1976;63:143–178. doi: 10.1007/978-3-642-66481-6_5. [DOI] [PubMed] [Google Scholar]
  12. Filipe M. I., Dawson I. The diagnostic value of mucosubstances in rectal biopsies from patients with ulcerative colitis and Crohn's disease. Gut. 1970 Mar;11(3):229–234. doi: 10.1136/gut.11.3.229. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Filipe M. I. The value of a study of the mucosubstances in rectal biopsies from patients with carcinoma of the rectum and lower sigmoid in the diagnosis of premalignant mucosa. J Clin Pathol. 1972 Feb;25(2):123–128. doi: 10.1136/jcp.25.2.123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Filipe M. I. Value of histochemical reactions for mucosubstances in the diagnosis of certain pathological conditions of the colon and rectum. Gut. 1969 Jul;10(7):577–586. doi: 10.1136/gut.10.7.577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Gad A. A histochemical study of human alimentary tract mucosubstances in health and disease. I. Normal and tumours. Br J Cancer. 1969 Mar;23(1):52–63. doi: 10.1038/bjc.1969.9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Gad A. A histochemical study of human alimentary tract mucosubstances in health and disease. II. Inflammatory conditions. Br J Cancer. 1969 Mar;23(1):64–68. doi: 10.1038/bjc.1969.10. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Gold D. V., Miller F. Characterization of human colonic mucoprotein antigen. Immunochemistry. 1974 Jul;11(7):369–375. doi: 10.1016/0019-2791(74)90190-6. [DOI] [PubMed] [Google Scholar]
  18. Goldman H., Ming S. C. Mucins in normal and neoplastic gastrointestinal epithelium. Histochemical distribution. Arch Pathol. 1968 Jun;85(6):580–586. [PubMed] [Google Scholar]
  19. Greco V., Lauro G., Fabbrini A., Torsoli A. Histochemistry of the colonic epithelial mucins in normal subjects and in patients with ulcerative colitis. A qualitative and histophotometric investigation. Gut. 1967 Oct;8(5):491–496. doi: 10.1136/gut.8.5.491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Hellstrom H. R., Fisher E. R. Estimation of mucosal mucin as an aid in the differentiation of Crohn's disease of the colon and chronic ulcerative colitis. Am J Clin Pathol. 1967 Sep;48(3):259–268. doi: 10.1093/ajcp/48.3.259. [DOI] [PubMed] [Google Scholar]
  21. Hradsky M., Langr F., Nerad V., Skaunic V. Alterations of the histological and histochemical pattern in ulcerative colitis biopsy specimens. Am J Proctol. 1967 Oct;18(5):392–398. [PubMed] [Google Scholar]
  22. Huizenga K. A. Colonic cancer in inflammatory bowel disease. Mayo Clin Proc. 1978 Jul;53(7):474–475. [PubMed] [Google Scholar]
  23. Johansen P. G., Kay R. Histochemistry of rectal mucus in cystic fibrosis of the pancreas. J Pathol. 1969 Dec;99(4):299–306. doi: 10.1002/path.1710990405. [DOI] [PubMed] [Google Scholar]
  24. Korhonen L. K., Mäkelä V., Lilius G. Carbohydrate-rich compounds in the colonic mucosa of man. II. Histochemical characteristics of colonic adenocarcinomas. Cancer. 1971 Jan;27(1):128–133. doi: 10.1002/1097-0142(197101)27:1<128::aid-cncr2820270119>3.0.co;2-#. [DOI] [PubMed] [Google Scholar]
  25. LEV R., SPICER S. S. A HISTOCHEMICAL COMPARISON OF HUMAN EPITHELIAL MUCINS IN NORMAL AND IN HYPERSECRETORY STATES INCLUDING PANCREATIC CYSTIC FIBROSIS. Am J Pathol. 1965 Jan;46:23–47. [PMC free article] [PubMed] [Google Scholar]
  26. Lev R., Orlic D. Histochemical and radioautographic studies of normal human fetal colon. Histochemistry. 1974 Jun 5;39(4):301–311. doi: 10.1007/BF00495681. [DOI] [PubMed] [Google Scholar]
  27. Mäkelä V., Korhonen L. K., Lilius G. Carbohydrate-rich compounds in the colonic mucosa of man. I. Histochemical characteristics of normal and adenomatous colonic mucosa. Cancer. 1971 Jan;27(1):120–127. doi: 10.1002/1097-0142(197101)27:1<120::aid-cncr2820270118>3.0.co;2-q. [DOI] [PubMed] [Google Scholar]
  28. Reid P. E., Culling C. F., Dunn W. L. A histochemical method for the identification of 9-O-acyl sialic acid. An investigation of bovine submaxillary gland and intestinal mucins. J Histochem Cytochem. 1978 Mar;26(3):187–192. doi: 10.1177/26.3.632555. [DOI] [PubMed] [Google Scholar]
  29. Reid P. E., Culling C. F., Dunn W. L., Clay M. G., Ramey C. W. A correlative chemical and histochemical study of the O-acetylated sialic acids of human colonic epithelial glycoproteins in formalin fixed paraffin embedded tissues. J Histochem Cytochem. 1978 Dec;26(12):1033–1041. doi: 10.1177/26.12.731017. [DOI] [PubMed] [Google Scholar]
  30. Reid P. E., Culling C. F., Dunn W. L., Clay M. G. The use of a transesterification technique to distinguish between certain neuraminidase resistant epithelial mucins. Histochemistry. 1976 Feb 26;46(3):203–207. doi: 10.1007/BF02462784. [DOI] [PubMed] [Google Scholar]
  31. Reid P. E., Culling C. F., Dunn W. L., Ramey C. W., Clay M. G. Differences in chemical composition between the epithelial glycoproteins of the upper and lower halves of rat colon. Can J Biochem. 1975 Dec;53(12):1328–1332. doi: 10.1139/o75-180. [DOI] [PubMed] [Google Scholar]
  32. Reid P. E., Culling C. F., Dunn W. L. Saponification-induced increase in the periodic acid-Schiff reaction in the gastrointestinal tract. Mechanism and distribution of the reactive substance. J Histochem Cytochem. 1973 May;21(5):473–482. doi: 10.1177/21.5.473. [DOI] [PubMed] [Google Scholar]
  33. Reid P. E., Culling C. F., Dunn W. L. The histochemical interpretation of the complex results of methylation upon gastrointestinal tract mucins, with special reference to the periodic acid-Schiff reactivity. J Histochem Cytochem. 1974 Oct;22(10):986–991. doi: 10.1177/22.10.986. [DOI] [PubMed] [Google Scholar]
  34. Reid P. E., Culling C. F., Ramey C. W., Dunn W. L., Clay M. G. A simple method for the determination of the O-acetyl substitution pattern of the sialic acids of colonic epithelial glycoprotein. Can J Biochem. 1977 May;55(5):493–503. doi: 10.1139/o77-070. [DOI] [PubMed] [Google Scholar]
  35. Reid P. E., Culling C. F., Tsang W. C., Clay M. G., Ramey C. W., Dunn W. L. The demonstration of O-acetylated sialic acids in colonic epithelial glycoproteins. Can J Biochem. 1975 Mar;53(3):388–391. doi: 10.1139/o75-053. [DOI] [PubMed] [Google Scholar]
  36. Subbuswamy S. G. Mucosubstances in neoplasms of the human colon and rectum. Gut. 1971 Mar;12(3):200–207. doi: 10.1136/gut.12.3.200. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Subbuswamy S. G. Patterns of mucin secretion in human intestinal mucosa. J Anat. 1971 Feb;108(Pt 2):291–293. [PMC free article] [PubMed] [Google Scholar]
  38. Van der Waaij D., Cohen B. J., Anver M. R. Mitigation of experimental inflammatory bowel disease in guinea pigs by selective elimination of the aerobic gram-negative intestinal microflora. Gastroenterology. 1974 Sep;67(3):460–472. [PubMed] [Google Scholar]
  39. Wood W. S., Culling C. F. Perianal Paget disease. Histochemical differentiation utilizing the borohydride-KOH-PAS reaction. Arch Pathol. 1975 Aug;99(8):442–445. [PubMed] [Google Scholar]

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