Abstract
Commercial 125I anti-HBs was processed to yield a sixfold improvement in economy without significant loss of sensitivity or specificity. Additional polystyrene beads were coupled with commercially supplied anti-HBs. The modified assay (Mod-RIA) was compared with commercial RIA, EIA, and RPHA using established HBsAg panels. Mod-RIA was also compared with HEPATEST (RPHA) for screening 71 200 fresh blood donations during an 11.5-month period.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Adachi H., Fukuda T., Funahashi S., Kurahori T., Ishikawa E. Sandwich enzymoimmunoassay of hepatitis B surface antigen (HBsAg). Vox Sang. 1978;35(4):219–223. doi: 10.1111/j.1423-0410.1978.tb02925.x. [DOI] [PubMed] [Google Scholar]
- Burrell C. J., Leadbetter G., Black S. H., Hunter W. M. Rapid detection of hepatitis B surface antigen by double antibody radioimmunoassay. J Med Virol. 1978;3(1):19–26. doi: 10.1002/jmv.1890030106. [DOI] [PubMed] [Google Scholar]
- Chrystie I. L., Islam M. N., Banatvala J. E. Clinical evaluation of the turkey-erythrocyte passive-haemagglutination test for hepatitis-B surface antigen. Lancet. 1974 Jun 15;1(7868):1193–1194. doi: 10.1016/s0140-6736(74)91003-4. [DOI] [PubMed] [Google Scholar]
- Hopkins R., Ross S., Jordan T., Watt A. D. Comparison of radioimmunoassay and enzyme immunoassay for detecting hepatitis B surface antigen in serum from freshly donated blood and selected blood products. J Clin Pathol. 1978 Oct;31(10):1000–1002. doi: 10.1136/jcp.31.10.1000. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kacaki J., Wolters G., Kuijpers L., Stulemeyer S. Results of a multicentre clinical trial of the solid-phase enzyme immunoassay for hepatitis B surface antigen. Vox Sang. 1978 Jul-Aug;35(1-2):65–74. doi: 10.1111/j.1423-0410.1978.tb02902.x. [DOI] [PubMed] [Google Scholar]
- Leers W. D., Kouroupis G. M. Comparison of the reversed passive hemagglutination with radioimmunoassay methods for hepatitis B antigen. J Clin Microbiol. 1975 Jul;2(1):8–10. doi: 10.1128/jcm.2.1.8-10.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ukkonen P., Koistinen V., Penttinen K. Enzyme-immunoassay in the detection of hepatitis B surface antigen. J Immunol Methods. 1977;15(4):343–353. doi: 10.1016/0022-1759(77)90096-5. [DOI] [PubMed] [Google Scholar]
- Watt A. D., Robertson M., Hopkins R. Technical method. Bulk preparation of reagents for hepatitis b testing. J Clin Pathol. 1976 Apr;29(4):364–366. doi: 10.1136/jcp.29.4.364. [DOI] [PMC free article] [PubMed] [Google Scholar]