Abstract
Using fresh clinical material, a comparison of a number of anaerobic selective media was made. For Gram-negative anaerobes nalidixic acid tween agar (NAT), neomycin agar (NA), and neomycin-vancomycin agar (NVA) all performed equally well. Kanamycin-containing media were more inhibitory to all Gram-negative anaerobes other than Bacteroides fragilis and B. melaninogenicus. When the recovery of Gram-positive anaerobes was examined NAT performed better than any of the other selective media used. No single selective medium could recover all anaerobes. Better isolation was achieved using a combination of two selective media (the best combinations being NAT and NVA or NAT and NA). Only a combination of three selective media gave the maximum recovery of anaerobes in this study (NAT, NVA, and NA or KA).
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Baldwin A. W. An improved catalyst sachet for anaerobic jars. Med Lab Technol. 1975 Oct;32(4):329–330. [PubMed] [Google Scholar]
- Bartlett J. G., Sullivan-Sigler N., Louie T. J., Gorbach S. L. Anaerobes survive in clinical specimens despite delayed processing. J Clin Microbiol. 1976 Feb;3(2):133–136. doi: 10.1128/jcm.3.2.133-136.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Killgore G. E., Starr S. E., Del Bene V. E., Whaley D. N., Dowell V. R., Jr Comparison of three anaerobic systems for the isolation of anaerobic bacteria from clinical specimens. Am J Clin Pathol. 1973 Apr;59(4):552–559. doi: 10.1093/ajcp/59.4.552. [DOI] [PubMed] [Google Scholar]
- Martin W. J. Isolation and indentification of anaerobic bacteria in the clinical laboratory. A 2-year experience. Mayo Clin Proc. 1974 May;49(5):300–308. [PubMed] [Google Scholar]
- Watt B., Collee J. G., Brown R. Tests of performance of anaerobic jars. J Clin Pathol. 1976 Jun;29(6):534–536. doi: 10.1136/jcp.29.6.534. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wren M. W. A new selective medium for the isolation of non-sporing anaerobic bacteria from clinical specimens. Med Lab Sci. 1978 Oct;35(4):371–378. [PubMed] [Google Scholar]
- Wren M. W., Baldwin A. W., Eldon C. P., Sanderson P. J. The anaerobic culture of clinical specimens: a 14-month study. J Med Microbiol. 1977 Feb;10(1):49–61. doi: 10.1099/00222615-10-1-49. [DOI] [PubMed] [Google Scholar]