REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
UCHT1 (BUV395) [Human anti-CD3]	BD Bioscience	Cat#563546; Lot: 1214287; RRID:AB_2744387
SK3 (BUV496) [Human anti-CD4]	BD Bioscience	Cat#612936; Lot: 2140305; RRID:AB_2870220
HI100 (BUV563) [Human anti-CD45RA]	BD Bioscience	Cat#612926; Lot: 1078217; RRID:AB_2870211
L128 (BUV661) [Human anti-CD27]	BD Bioscience	Ca#750167; Lot: 3021264; RRID:AB_2874372
NK-1 (BV421) [Human anti-CD57]	Biolegend	Cat#393326; Lot: B361783 ; RRID:AB_2860964
M5E2 (BV480) [Human anti-CD14]	BD Bioscience	Cat#746304; Lot 2286909; RRID:AB_2743629
HIB19 (BV480) [Human anti-CD19]	BD Bioscience	Cat#746457; Lot: 2286911; RRID:AB_2743759
RPA-T8 (BV570) [Human anti-CD8]	Biolegend	Cat#301038; Lot: B346256; RRID:AB_2563213
H4A3 (BV786) [Humananti-CD107a]	BD Bioscience	Cat#563869; Lot: 1207206; RRID:AB_2738458
Mab11 (AF488) [Human anti-TNF⍺]	Biolegend	Cat#502915; Lot: B311742; RRID:AB_493121
FN50 (PerCPeF710) [Human anti-CD69]	ebioscience	Cat#46-0699-42; Lot: 2514279; RRID:AB_2573694
REA1063 (PE) [Human anti- IL17a]	Miltenyi	Cat#130-118-243; Lot: 1323010976; RRID:AB_2751466
MQ1-17H12 (PE-Dazzle594) [Human anti-IL-2]	Biolegend	Cat#500344; Lot:B351592; RRID:AB_2564091
(PE-Cy7) [Human anti-IFNγ]	BD Bioscience	Cat#557643; Lot: 1229911; RRID:AB_396760
2-L1-A (APC-R700) [Human anti-CCR7]	BD Bioscience	Cat#566766; Lot: 2257402; RRID:AB_2869856
TRAP1 (APC) [Human anti-CD40L]	BD Bioscience	Cat#555702; Lot: 2010706; RRID:AB_398610
LIVE/DEAD Fixable dead cell	BD Bioscience	Cat#L34960 ;Lot:2433781
CD28.2 (BUV737) [Human anti-CD28]	BD Bioscience	Cat#612815; Lot: 2251073; RRID:AB_2870140
EH12.2 (BB515) [Human anti-PD-1]	BD Bioscience	Cat#564494; Lot: 2101875; RRID:AB_2738827
FN50 (BV650) [Human anti-CD69]	Biolegend	Cat#310934; Lot:B360356; RRID:AB_2563158
MBSA43 (PerCPeF710) [Human anti-TIGIT]	Invitrogen ebioscience	Cat#46-9500-42; Lot:2402674; RRID:AB_10853679
4B4-1 (PE-Dazzle594) [Human anti-4-1BB]	Biolegend	Cat#309826; Lot:B349385; RRID:AB_2566259
ISA-3 (PE-Cy7) [Human anti-ICOS]	Invitrogen ebioscience	Cat#25-9948-42; Lot: 2338678; RRID:AB_1518754
ACT35 (APC) [Human anti-OX40]	BD Bioscience	Cat# 563473; Lot: 2026053; RRID:AB_2738230
TRAP (PE) [Human anti-CD40L]	BD Bioscience	Cat#555700; Lot: 0357889; RRID:AB_396050
CD3 Antibody, anti-human, Biotin functional grade	Miltenyi	Cat#130-093-377; Lot: 5210202091; RRID:AB_1036126
Biotin anti-human CD28	Biolegend	Cat:302904; Lot: B394472; RRID:AB_314306
Biotin Mouse IgG1, κ Isotype Ctrl Antibody	Biolegend	Cat#400104; Lot: B350305; RRID:AB_326427
Purified Goat anti-mouse IgG (minimal x-reactivity) Antibody	Biolegend	Cat# 405301; Lot: B343022 RRID:AB_315005
Chemicals, peptides, and recombinant proteins
Staphylococcal Enterotoxin B (SEB)	Toxin technology	Cat#BT202
Silica microspheres	Bangs Laboratoreis	Cat# SS05003 ; Lot: 16497
Srteptavidin	Jackson ImmunoResearch	Cat#016-000-084; Lot: 154826
18:1 (Δ9-Cis) PC (DOPC)	Avanti polar (via sigma)	Cat# 850375C-200mg
18:1 DGS-NTA(Ni)	Avanti polar (via sigma)	Cat# 790404C-10mg
18:1 Biotinyl Cap PE	Avanti polar (via sigma)	Cat#870273C-25mg
RPMI 1640	GIBCO by Life Technologies	Cat# 11875-119
HEPES	Invitrogen	Cat# 15630-080
Penicillin-Streptomycin	GIBCO by Life Technologies	Cat# 15140-122
Nuclease S7 from Staphylococcus aureus	Roche Diagnostics	Cat# 12344000
Bovine Serum Albumin	Sigma-Aldrich	Cat#A2153-50G CAS#9048-46-8
BD GolgiStop (Monensin)	BD Bioscience	Cat# 554724
BD GolgiPlug (Brefeldin A)	BD Bioscience	Cat#555029
FcR Blocking Reagent, human	Miltenyi	Cat#130-059-901
Invitrogen™ UltraPure™ Tris Buffer (powder format)	Invitrogen	Cat#15504020
Critical commercial assays
EasySep Human T Cell Enrichment Kit	StemCell	Cat# 19051_C
Dynabeads Human T-Activator CD3/CD28 - for physiological activation of human T cells	ThermoFisher	Cat# 11161D; Lot:2860963; RRID:AB_2916088
Deposited data
Original code is deposited at	N/A	“Original Code : https://github.com/alemi055/scripts-and-data/blob/master/ShaabanKabakiboetal_2024.R”
Software and algorithms
Flow Jo v10.8.0	Flow Jo	https://www.flowjo.com
GraphPad Prism v8.4.1	GraphPad	https://www.graphpad.com