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Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1980 Apr;33(4):377–379. doi: 10.1136/jcp.33.4.377

Quantitation of oestrogen receptors: use of solid-phase antisteroid antibodies to quantify binding sites and determination of dissociation constant.

S Raam, J L Cohen
PMCID: PMC1146083  PMID: 7400336

Abstract

The dextran-coated charcoal assay method of McGuire and DeLaGarza is a well-known method for quantitation of oestrogen receptors in breast tumour tissues. Castañeda and Liao reported that the solid-phase antisteroid antibodies are superior to dextran-coated charcoal for quantitating the receptor bound steroids. In this report we compare the results obtained using both methods to quantitate oestrogen receptors and determine the affinity for steroid binding in seven human breast tumours and the uteri of rat and calf.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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