Abstract
The use of lyphogel to concentrate the number of virus particles in specimens for electron microscopic examination was studied in parallel with ultracentrifugation. One hundred faecal and urine samples were compared. Both methods had a similar sensitivity. Lyphogel was economical, simple, and rapid in use; in contrast to ultracentrifugation, it required relatively little material. The procedure could be done within a safety cabinet, and virus particles were morphologically undamaged by the process.
Full text
PDF



Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Ashcavai M., Peters R. L. Hepatitis-associated antigen: improved sensitivity in detection. Am J Clin Pathol. 1971 Mar;55(3):262–268. doi: 10.1093/ajcp/55.3.262. [DOI] [PubMed] [Google Scholar]
- Bishop R. F., Davidson G. P., Holmes I. H., Ruck B. J. Detection of a new virus by electron microscopy of faecal extracts from children with acute gastroenteritis. Lancet. 1974 Feb 2;1(7849):149–151. doi: 10.1016/s0140-6736(74)92440-4. [DOI] [PubMed] [Google Scholar]
- Flewett T. H., Bryden A. S., Davies H. Diagnostic electron microscopy of faeces. I. The viral flora of the faeces as seen by electron microscopy. J Clin Pathol. 1974 Aug;27(8):603–608. doi: 10.1136/jcp.27.8.603. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kapikian A. Z., Gerin J. L., Wyatt R. G., Thornhill T. S., Chanock R. M. Density in cesium chloride of the 27 nm "8FIIa" particle associated with acute infectious nonbacterial gastroenteritis: determination by ultra-centrifugation and immune electron microscopy. Proc Soc Exp Biol Med. 1973 Mar;142(3):874–877. doi: 10.3181/00379727-142-37135. [DOI] [PubMed] [Google Scholar]
- Kapikian A. Z., Wyatt R. G., Dolin R., Thornhill T. S., Kalica A. R., Chanock R. M. Visualization by immune electron microscopy of a 27-nm particle associated with acute infectious nonbacterial gastroenteritis. J Virol. 1972 Nov;10(5):1075–1081. doi: 10.1128/jvi.10.5.1075-1081.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Madeley C. R. Viruses in the stools. J Clin Pathol. 1979 Jan;32(1):1–10. doi: 10.1136/jcp.32.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Solaas M. H. Comparison of small hepatitis B surface antigen particles and human serum low density lipoprotein molecules by electron microscopy. Acta Pathol Microbiol Scand B. 1978 Jun;86B(3):125–129. doi: 10.1111/j.1699-0463.1978.tb00020.x. [DOI] [PubMed] [Google Scholar]