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. 1980 Jul;33(7):609–616. doi: 10.1136/jcp.33.7.609

Immunoperoxidase staining of surface and intracellular immunoglobulin in human neoplastic lymphoid cells.

D Y Mason, R C Leonard, G Laurent, M F Gourdin
PMCID: PMC1146170  PMID: 7000833

Abstract

An immunoperoxidase technique for the optical microscopic detection of cellular immunoglobulin has been used to stain fixed smears of human neoplastic B lymphoid cells. Only four out of 28 cases of chronic lymphatic leukaemia (CLL) showed membrane labelling by this technique. In contrast, when 14 samples from other types of B lymphoproliferative disorder (including hairy cell leukaemia, non-Hodgkin's lymphoma, and prolymphocytic leukaemia) were studied, all samples showed membrane immunoglobulin labelling (confirmed by capping experiments). This discrepancy was attributed to the greater density of surface immunoglobulin present on neoplastic cells in the latter group of disorders compared to CLL. This immunoperoxidase technique is therefore less sensitive than immunofluorescent staining of cells in suspension for the demonstration of neoplastic cell surface immunoglobulin. However, it offers a number of advantages (eg, excellent visualisation of cell morphology, permanence of stained preparations, and applicability to stored samples) which recommend it as the method of choice in certain clinical haematological contexts.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aisenberg A. C., Wilkes B. Lymphosarcoma cell leukemia: the contribution of cell surface study to diagnosis. Blood. 1976 Nov;48(5):707–715. [PubMed] [Google Scholar]
  2. Brown G., Biberfeld P., Christensson B., Mason D. Y. The distribution of HLA on human lymphoid, bone marrow and peripheral blood cells. Eur J Immunol. 1979 Apr;9(4):272–275. doi: 10.1002/eji.1830090405. [DOI] [PubMed] [Google Scholar]
  3. Cawley J. C., Smith J., Goldstone A. H., Emmines J., Hamblin J., Hough L. IgA and IgM cytoplastic inclusions in a series of cases of chronic lymphocytic leukaemia. Clin Exp Immunol. 1976 Jan;23(1):78–82. [PMC free article] [PubMed] [Google Scholar]
  4. Clark C., Rydell R. E., Kaplan M. E. Frequent association of IgMlambda with crystalline inclusions in chronic lymphatic leukemic lymphocytes. N Engl J Med. 1973 Jul 19;289(3):113–117. doi: 10.1056/NEJM197307192890301. [DOI] [PubMed] [Google Scholar]
  5. Cooper A. G., Brown M. C., Derby H. A., Wortis H. H. Quantitation of surface-membrane and intracellular gamma, mu and kappa chains of normal and neoplastic human lymphocytes. Clin Exp Immunol. 1973 Apr;13(4):487–496. [PMC free article] [PubMed] [Google Scholar]
  6. Dighiero G., Follezou J. Y., Roisin J. P., Ternynck T., Binet J. L. Comparison of normal and chronic lymphocytic leukemia lymphocyte surface Ig determinants using peroxidase-labeled antibodies. II. quantification of light chain determinants in atypical lymphocytic leukemia. Blood. 1976 Oct;48(4):559–566. [PubMed] [Google Scholar]
  7. Hijmans W., Schuit H. R., Klein F. An immunofluorescence procedure for the detection of intracellular immunoglobulins. Clin Exp Immunol. 1969 Apr;4(4):457–472. [PMC free article] [PubMed] [Google Scholar]
  8. Hurez D., Flandrin G., Preud'homme J. L., Seligmann M. Unreleased intracellular monoclonal macroglobulin in chronic lymphocytic leukaemia. Clin Exp Immunol. 1972 Feb;10(2):223–234. [PMC free article] [PubMed] [Google Scholar]
  9. Mason D. Y., Labaume S., Preud'homme J. L. The detection of membrane and cytoplasmic immunoglobulins in human leucocytes by immunoperoxidase staining. Clin Exp Immunol. 1977 Sep;29(3):413–421. [PMC free article] [PubMed] [Google Scholar]
  10. McKeever P. E., Garvin A. J., Spicer S. S. Immune complex receptors on cell surface. I. Ultrastructural demonstration of macrophages. J Histochem Cytochem. 1976 Aug;24(8):948–955. doi: 10.1177/24.8.60441. [DOI] [PubMed] [Google Scholar]
  11. McMichael A. J., Pilch J. R., Galfré G., Mason D. Y., Fabre J. W., Milstein C. A human thymocyte antigen defined by a hybrid myeloma monoclonal antibody. Eur J Immunol. 1979 Mar;9(3):205–210. doi: 10.1002/eji.1830090307. [DOI] [PubMed] [Google Scholar]
  12. Preud'homme J. L., Seligmann M. Surface immunoglobulins on human lymphoid cells. Prog Clin Immunol. 1974;2:121–174. [PubMed] [Google Scholar]
  13. Zidar B. L., Winkelstein A., Whiteside T. L., Shadduck R. K., Zeigler Z., Smith W. I., Rabin B. S., Krause J. R., Lee R. E. Hairy cell leukaemia: seven cases with probable B-lymphocytic origin. Br J Haematol. 1977 Dec;37(4):455–465. doi: 10.1111/j.1365-2141.1977.tb01018.x. [DOI] [PubMed] [Google Scholar]

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