Abstract
Neutrophil candidacidal function was measured by a chromium-51 (51Cr) release technique, using Candida guilliermondii as the test organism. The effect of varying the neutrophil:candida ratio, the time course of 51Cr release, the effect of different serum concentrations, and the reproducibility of the technique were investigated. Results from 33 experiments, using neutrophils from 35 normal donors and six patients with known neutrophil defects, show the technique to be a reliable and reproducible assay of candida killing. The chromium release assay has the advantage of being easy to perform and not liable to subjective errors.
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- Child J. A., Cawley J. C., Martin S., Ghoneim A. T. Microbicidal function of the neutrophils in hairy-cell leukaemia. Acta Haematol. 1979;62(4):191–198. doi: 10.1159/000207571. [DOI] [PubMed] [Google Scholar]
- Child J. A., Martin S., Cawley J. C., Ghoneim A. T. Defective microbicidal function of neutrophils in haematological malignancies and lymphomas: correction by levamisole in vitro. Biomedicine. 1978 Jul;29(5):159–161. [PubMed] [Google Scholar]
- Chilgren R. A., Hong R., Quie P. G. Human serum interactions with Candida albicans. J Immunol. 1968 Jul;101(1):128–132. [PubMed] [Google Scholar]
- El-Maalem H., Fletcher J. Defective neutrophil function in chronic granulocytic leukaemia. Br J Haematol. 1976 Sep;34(1):95–103. doi: 10.1111/j.1365-2141.1976.tb00178.x. [DOI] [PubMed] [Google Scholar]
- El-Maallem H., Fletcher J. Defective hydrogen peroxide production in chronic granulocytic leukaemia neutrophils. Br J Haematol. 1979 Jan;41(1):49–55. doi: 10.1111/j.1365-2141.1979.tb03680.x. [DOI] [PubMed] [Google Scholar]
- GOLDSTEIN E., GRIECO M. H., FINKEL G., LOURIA D. B. STUDIES ON THE PATHOGENESIS OF EXPERIMENTAL CANDIDA PARAPSILOSIS AND CANDIDA GUILLIERMONDII INFECTIONS IN MICE. J Infect Dis. 1965 Jun;115:293–302. doi: 10.1093/infdis/115.3.293. [DOI] [PubMed] [Google Scholar]
- Holmes B., Page A. R., Good R. A. Studies of the metabolic activity of leukocytes from patients with a genetic abnormality of phagocytic function. J Clin Invest. 1967 Sep;46(9):1422–1432. doi: 10.1172/JCI105634. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Houghton J. B. Neutrophil function and diagnosis of pre-leukaemic states. Postgrad Med J. 1979 Jun;55(644):396–399. doi: 10.1136/pgmj.55.644.396. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lehrer R. I. Measurement of candidacidal activity of specific leukocyte types in mixed cell populations I. Normal, myeloperoxidase-deficient, and chronic granulomatous disease neutrophils. Infect Immun. 1970 Jul;2(1):42–47. doi: 10.1128/iai.2.1.42-47.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rosner F., Valmont I., Kozinn P. J., Caroline L. Leukocyte function in patients with leukemia. Cancer. 1970 Apr;25(4):835–842. doi: 10.1002/1097-0142(197004)25:4<835::aid-cncr2820250412>3.0.co;2-#. [DOI] [PubMed] [Google Scholar]
- Yamamura M., Boler J., Valdimarsson H. A chromium release assay for phagocytic killing of Candida albicans. J Immunol Methods. 1976;13(3-4):227–233. doi: 10.1016/0022-1759(76)90069-7. [DOI] [PubMed] [Google Scholar]