Abstract
A number of fixatives were tested to determine their suitability for use with the unlabelled antibody peroxidase-antiperoxidase (PAP) method for demonstrating immunoglobulin in paraffin sections of tonsil and trephine samples of bone marrow. It was found that tonsil fixed in 'isotonic' solutions of formaldehyde reacted with the PAP method only after the sections had been trypsinised. Several other fixatives, including Bouin's fluid, Carnoy's fluid, and solutions containing mercuric chloride, gave tissues which reacted without trypsinisation of sections, and particularly good results were obtained with formol saline to which acetic acid (2-10%) had been added. A combination of acetic acid (10%)-formol saline and formol sublimate also gave excellent results with bone marrow. The influence on the PAP method of a number of steps in the processing of tissues and sections was also examined.
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- Bosman F. T., Lindeman J., Kuiper G., van der Wal A., Kreunig J. The influence of fixation on immunoperoxidase staining of plasmacells in paraffin sections of intestinal biopsy specimens. Histochemistry. 1977 Jul 18;53(1):57–62. doi: 10.1007/BF00511210. [DOI] [PubMed] [Google Scholar]
- Carson F. L., Martin J. H., Lynn J. A. Formalin fixation for electron microscopy: a re-evaluation. Am J Clin Pathol. 1973 Mar;59(3):365–373. doi: 10.1093/ajcp/59.3.365. [DOI] [PubMed] [Google Scholar]
- Curran R. C., Gregory J. Demonstration of immunoglobulin in cryostat and paraffin sections of human tonsil by immunofluorescence and immunoperoxidase techniques. Effects of processing on immunohistochemical performance of tissues and on the use of proteolytic enzymes to unmask antigens in sections. J Clin Pathol. 1978 Oct;31(10):974–983. doi: 10.1136/jcp.31.10.974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Curran R. C., Gregory J. The unmasking of antigens in paraffin sections of tissue by trypsin. Experientia. 1977 Oct 15;33(10):1400–1401. doi: 10.1007/BF01920206. [DOI] [PubMed] [Google Scholar]
- Curran R. C., Jones E. L. Immunoglobulin-containing cells in human tonsils as demonstrated by immunohistochemistry. Clin Exp Immunol. 1977 Apr;28(1):103–115. [PMC free article] [PubMed] [Google Scholar]
- Heyderman E. Immunoperoxidase technique in histopathology: applications, methods, and controls. J Clin Pathol. 1979 Oct;32(10):971–978. doi: 10.1136/jcp.32.10.971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Robinson G., Dawson I. A formalin fixative for immunochemical and ultrastructural studies on gastrointestinal endocrine cells. J Clin Pathol. 1979 Jan;32(1):40–45. doi: 10.1136/jcp.32.1.40. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sternberger L. A., Hardy P. H., Jr, Cuculis J. J., Meyer H. G. The unlabeled antibody enzyme method of immunohistochemistry: preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem. 1970 May;18(5):315–333. doi: 10.1177/18.5.315. [DOI] [PubMed] [Google Scholar]
- Taylor C. R., Burns J. The demonstration of plasma cells and other immunoglobulin-containing cells in formalin-fixed, paraffin-embedded tissues using peroxidase-labelled antibody. J Clin Pathol. 1974 Jan;27(1):14–20. doi: 10.1136/jcp.27.1.14. [DOI] [PMC free article] [PubMed] [Google Scholar]