Figure 5: Loss of RECQL5 modestly impairs RAG-DSB repair in Setx^−/− abl pre-B cells.

(A) Western blot analysis of cell lysates from WT and three independently isolated Recql5^−/− abl pre-B cells using RECQL5 and GAPDH antibodies. (B) Flow cytometric analysis for GFP expression in cells described in (A) with pMG-INV and treated with imatinib (imat.) for the indicated times. The percentages of GFP⁺ cells are indicated in the top left corners of the histograms. (C) Western blot analysis of cell lysates from Setx^−/− and three independently isolated Setx^−/−: Recql5^−/− abl pre-B cells using RECQL5 and GAPDH antibodies. (D) Flow cytometric analysis for GFP expression in cells described in (C) treated with imatinib for the indicated times. (E) Southern blot analysis of EcoRV-digested genomic DNA from cells described in (D) with retroviral pMX-DEL^SJ and treated with imatinib (imat.) in the presence or absence of the DNA-PKcs kinase inhibitor NU7441 for the indicated times.