Figure 8: Partial inhibition of RNA polymerase II improves NHEJ-mediated RAG DSB repair in helicase-deficient abl pre-B cells.

Southern blot analysis of EcoRV-digested genomic DNA from pMX-DEL^SJ containing, imatinib (imat.) treated Setx^−/− (with the DNA-PKcs kinase inhibitor NU7441) (A), Setx^−/−: Recql5^−/−: Hltf^−/− (B) and Recql5^−/−: Hltf^−/− (with the ATM kinase inhibitor KU55933) (C) abl pre-B cells with increasing concentrations of the CDK7 inhibitor THZ1 for the indicated times. The percentages of unrepaired (full length and resected) SEs in total RAG cleaved recombination substrates (SE+SJ) are shown below the Southern blot images.