Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1986 Mar 1;234(2):475–479. doi: 10.1042/bj2340475

Changes in mouse intervertebral-disc proteoglycan synthesis with age. Hereditary kyphoscoliosis is associated with elevated synthesis.

G Venn, R M Mason
PMCID: PMC1146590  PMID: 3718480

Abstract

Mice with hereditary kyphoscoliosis (ky/ky) develop intervertebral-disc degeneration at the cervico-thoracic junction. Disc proteoglycans were investigated to determine whether changes in synthesis or structure were associated with this. Elevated 35S-proteoglycan synthesis was found in one or more cervico-thoracic discs in 80-day-old ky/ky mice. The hydrodynamic size and aggregation properties of ky/ky-mouse disc 35S-proteoglycans extracted with 4 M-guanidinium chloride were normal. Increased proportions of small 35S-proteoglycans were extracted with 0.5 M-guanidinium chloride from discs of normal and ky/ky mice with increasing age.

Full text

PDF
475

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams P., Muir H. Qualitative changes with age of proteoglycans of human lumbar discs. Ann Rheum Dis. 1976 Aug;35(4):289–296. doi: 10.1136/ard.35.4.289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dickinson A. G., Meikle V. M. Genetic kyphoscoliosis in mice. Lancet. 1973 May 26;1(7813):1186–1186. doi: 10.1016/s0140-6736(73)91186-0. [DOI] [PubMed] [Google Scholar]
  3. Faltz L. L., Reddi A. H., Hascall G. K., Martin D., Pita J. C., Hascall V. C. Characteristics of proteoglycans extracted from the Swarm rat chondrosarcoma with associative solvents. J Biol Chem. 1979 Feb 25;254(4):1375–1380. [PubMed] [Google Scholar]
  4. Ghosh P., Taylor T. K., Braund K. G. The variation of the glycosaminoglycans of the canine intervertebral disc with ageing. I. Chondrodystrophoid breed. Gerontology. 1977;23(2):87–98. doi: 10.1159/000212177. [DOI] [PubMed] [Google Scholar]
  5. Lipson S. J., Muir H. 1980 Volvo award in basic science. Proteoglycans in experimental intervertebral disc degeneration. Spine (Phila Pa 1976) 1981 May-Jun;6(3):194–210. doi: 10.1097/00007632-198105000-00002. [DOI] [PubMed] [Google Scholar]
  6. Lyons G., Eisenstein S. M., Sweet M. B. Biochemical changes in intervertebral disc degeneration. Biochim Biophys Acta. 1981 Apr 3;673(4):443–453. doi: 10.1016/0304-4165(81)90476-1. [DOI] [PubMed] [Google Scholar]
  7. Mason R. M., Palfrey A. J. Intervertebral disc degeneration in adult mice with hereditary kyphoscoliosis. J Orthop Res. 1984;2(4):333–338. doi: 10.1002/jor.1100020405. [DOI] [PubMed] [Google Scholar]
  8. Oegema T. R., Jr, Hascall V. C., Dziewiatkowski D. D. Isolation and characterization of proteoglycans from the swarm rat chondrosarcoma. J Biol Chem. 1975 Aug 10;250(15):6151–6159. [PubMed] [Google Scholar]
  9. Oohira A., Nogami H. Age-related changes in physical and chemical properties of proteoglycans synthesized by costal and matrix-induced cartilages in the rat. J Biol Chem. 1980 Feb 25;255(4):1346–1350. [PubMed] [Google Scholar]
  10. Pedrini-Mille A., Pedrini V. A., Tudisco C., Ponseti I. V., Weinstein S. L., Maynard J. A. Proteoglycans of human scoliotic intervertebral disc. J Bone Joint Surg Am. 1983 Jul;65(6):815–823. [PubMed] [Google Scholar]
  11. Roughley P. J., White R. J. Age-related changes in the structure of the proteoglycan subunits from human articular cartilage. J Biol Chem. 1980 Jan 10;255(1):217–224. [PubMed] [Google Scholar]
  12. Sandy J. D., Adams M. E., Billingham M. E., Plaas A., Muir H. In vivo and in vitro stimulation of chondrocyte biosynthetic activity in early experimental osteoarthritis. Arthritis Rheum. 1984 Apr;27(4):388–397. doi: 10.1002/art.1780270405. [DOI] [PubMed] [Google Scholar]
  13. Simůnek Z., Muir H. Changes in the protein-polysaccharides of pig articular cartilage during prenatal life, development and old age. Biochem J. 1972 Feb;126(3):515–523. doi: 10.1042/bj1260515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Stevens R. L., Ewins R. J., Revell P. A., Muir H. Proteoglycans of the intervertebral disc. Homology of structure with laryngeal proteoglycans. Biochem J. 1979 Jun 1;179(3):561–572. doi: 10.1042/bj1790561. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Venn G., Mason R. M. Absence of keratan sulphate from skeletal tissues of mouse and rat. Biochem J. 1985 Jun 1;228(2):443–450. doi: 10.1042/bj2280443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Venn G., Mason R. M. Biosynthesis and metabolism in vivo of intervertebral-disc proteoglycans in the mouse. Biochem J. 1983 Nov 1;215(2):217–225. doi: 10.1042/bj2150217. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES