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. 1986 Apr 1;235(1):49–55. doi: 10.1042/bj2350049

Purification of a liver DNA-synthesis promoter from plasma of partially hepatectomized rats.

J J Díaz-Gil, P Escartín, R García-Cañero, C Trilla, J J Veloso, G Sánchez, A Moreno-Caparrós, C Enrique de Salamanca, R Lozano, J G Gavilanes, et al.
PMCID: PMC1146647  PMID: 3741389

Abstract

A protein was isolated from plasma of partially (70%) hepatectomized rats that, injected in mice, increases the uptake of [3H]thymidine by liver DNA by 200-300% over that by injected control saline. The purification procedure consists essentially of three chromatography steps, employing Sephadex G-75, DEAE-cellulose and hydroxyapatite. The hepatic promoter (HP) preparation shows a single band in SDS/polyacrylamide (15%)-gel electrophoresis (silver stained), with an Mr of 64 000; its activity is suppressed by trypsin or pepsin and is unaffected by deoxyribonuclease or ribonuclease. On injection into mice (150 ng/mouse), it increases the mitotic index of the liver. It shows organ-specificity, acting on liver but not on spleen, kidney, lung or brain. In primary liver cultures, it produces an increase in uptake of [3H]thymidine into DNA in the range 1-10 ng/ml. In this system in vitro, it increases the uptake of 22Na+ immediately after addition.

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