Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1986 Apr 15;235(2):613–615. doi: 10.1042/bj2350613

Progress-curve analysis in enzyme kinetics. Numerical solution of integrated rate equations.

R G Duggleby
PMCID: PMC1146728  PMID: 3741409

Abstract

Progress curves of enzyme-catalysed reactions are described by equations of a type that precludes direct calculation of the extent of reaction at any time. Previously, such equations have been solved by the Newton-Raphson method, but this procedure may fail when based upon the usual formulae. An alternative formulation is proposed that is both quicker and more robust.

Full text

PDF
613

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Darvey I. G., Shrager R., Kohn L. D. Integrated steady state rate equations and the determination of individual rate constants. J Biol Chem. 1975 Jun 25;250(12):4696–4701. [PubMed] [Google Scholar]
  2. Duggleby R. G., Morrison J. F. Progress curve analysis in enzyme kinetics: model discrimination and parameter estimation. Biochim Biophys Acta. 1978 Oct 12;526(2):398–409. doi: 10.1016/0005-2744(78)90131-6. [DOI] [PubMed] [Google Scholar]
  3. Duggleby R. G., Morrison J. F. The analysis of progress curves for enzyme-catalysed reactions by non-linear regression. Biochim Biophys Acta. 1977 Apr 12;481(2):297–312. doi: 10.1016/0005-2744(77)90264-9. [DOI] [PubMed] [Google Scholar]
  4. Fernley H. N. Statistical estimations in enzyme kinetics. The integrated Michaelis equation. Eur J Biochem. 1974 Apr 1;43(2):377–378. doi: 10.1111/j.1432-1033.1974.tb03423.x. [DOI] [PubMed] [Google Scholar]
  5. Kellershohn N., Laurent M. Analysis of progress curves for a highly concentrated Michaelian enzyme in the presence or absence of product inhibition. Biochem J. 1985 Oct 1;231(1):65–74. doi: 10.1042/bj2310065. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES