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. 1986 Jun 1;236(2):601–603. doi: 10.1042/bj2360601

The nucleotide sequence of a cytoplasmic tRNAPhe from Scenedesmus obliquus and comparison with a tRNATyr species.

G A Green, D S Jones
PMCID: PMC1146882  PMID: 3638966

Abstract

The nucleotide sequence of a cytoplasmic tRNAPhe from the eukaryotic green alga Scenedesmus obliquus was determined as: pG-G-C-U-U-G-A-U-A-m2G-C-U-C-A-G-C-D-Gm-G-G-A-G-A-G-C-m22G-p si-psi-A-G-A-Cm-U-G - A-A-m1G-A-psi-C-U-A-C-A-G-m7G-N-m5C-C-C-C-A-G-T-psi-C-G-m1A-U-m5C-Cm-U-G -G-G-U- C A-G-G-C-C-A-C-C-A-OH. The structure has some notable features. Unlike other tRNAPhe species from plant sources, it has an unmodified G as the first residue of the anticodon and m1G rather than a Y derivative as the residue following the anticodon. The sequence m5C(60)-Cm(61) is unique to this tRNA. The sequence of S. obliquus tRNAPhe shows close homology with S. obliquus tRNATyr.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Donis-Keller H., Maxam A. M., Gilbert W. Mapping adenines, guanines, and pyrimidines in RNA. Nucleic Acids Res. 1977 Aug;4(8):2527–2538. doi: 10.1093/nar/4.8.2527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Green G. A., Jones D. S. The nucleotide sequences of a cytoplasmic and a chloroplast tRNATyr from Scenedesmus obliquus. Nucleic Acids Res. 1985 Mar 11;13(5):1659–1663. doi: 10.1093/nar/13.5.1659. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Holmquist R., Jukes T. H., Pangburn S. Evolution of transfer RNA. J Mol Biol. 1973 Jun 25;78(1):91–116. doi: 10.1016/0022-2836(73)90430-0. [DOI] [PubMed] [Google Scholar]
  4. KIRBY K. S. ISOLATION AND CHARACTERIZATION OF RIBOSOMAL RIBONUCLEIC ACID. Biochem J. 1965 Jul;96:266–269. doi: 10.1042/bj0960266. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lake J. A. Aminoacyl-tRNA binding at the recognition site is the first step of the elongation cycle of protein synthesis. Proc Natl Acad Sci U S A. 1977 May;74(5):1903–1907. doi: 10.1073/pnas.74.5.1903. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. McCoy J. M., Jones D. S. The nucleotide sequence of Scenedesmus obliquus chloroplast tRNAfMet. Nucleic Acids Res. 1980 Nov 11;8(21):5089–5093. doi: 10.1093/nar/8.21.5089. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Miller J. P., Hussain Z., Schweizer M. P. The involvement of the anticodon adjacent modified nucleoside N-(9-(BETA-D-ribofuranosyl) purine-6-ylcarbamoyl)-threonine in the biological function of E. coli tRNAile. Nucleic Acids Res. 1976 May;3(5):1185–1201. doi: 10.1093/nar/3.5.1185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Nishimura S. Minor components in transfer RNA: their characterization, location, and function. Prog Nucleic Acid Res Mol Biol. 1972;12:49–85. [PubMed] [Google Scholar]
  9. Olins P. O., Jones D. S. Nucleotide sequence of Scenedesmus obliquus cytoplasmic initiator tRNA. Nucleic Acids Res. 1980 Feb 25;8(4):715–729. [PMC free article] [PubMed] [Google Scholar]
  10. Silberklang M., Gillum A. M., RajBhandary U. L. Use of in vitro 32P labeling in the sequence analysis of nonradioactive tRNAs. Methods Enzymol. 1979;59:58–109. doi: 10.1016/0076-6879(79)59072-7. [DOI] [PubMed] [Google Scholar]
  11. Sprinzl M., Moll J., Meissner F., Hartmann T. Compilation of tRNA sequences. Nucleic Acids Res. 1985;13 (Suppl):r1–49. doi: 10.1093/nar/13.suppl.r1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Stanley J., Vassilenko S. A different approach to RNA sequencing. Nature. 1978 Jul 6;274(5666):87–89. doi: 10.1038/274087a0. [DOI] [PubMed] [Google Scholar]

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