Abstract
To overcome the limit of the intensity of ions carrying sequence information in structural determinations of peptides by fast-atom-bombardment m.s., we have developed a method that consists in taking spectra of the peptide acid hydrolysates at different hydrolysis times. Peaks correspond to the oligomers arising from the peptide partial hydrolysis. The sequence can then be identified from the structurally overlapping fragments.
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Selected References
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- Barber M., Bordoli R. S., Garner G. V., Gordon D. B., Sedgwick R. D., Tetler L. W., Tyler A. N. Fast-atom-bombardment mass spectra of enkephalins. Biochem J. 1981 Aug 1;197(2):401–404. doi: 10.1042/bj1970401. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hill R. L. Hydrolysis of proteins. Adv Protein Chem. 1965;20:37–107. doi: 10.1016/s0065-3233(08)60388-5. [DOI] [PubMed] [Google Scholar]
- Morris H. R., Panico M., Taylor G. W. FAB-mapping of recombinant-DNA protein products. Biochem Biophys Res Commun. 1983 Nov 30;117(1):299–305. doi: 10.1016/0006-291x(83)91575-9. [DOI] [PubMed] [Google Scholar]
- Williams D. H., Bradley C. V., Santikarn S., Bojesen G. Fast-atom-bombardment mass spectrometry. A new technique for the determination of molecular weights and amino acid sequences of peptides. Biochem J. 1982 Jan 1;201(1):105–117. doi: 10.1042/bj2010105. [DOI] [PMC free article] [PubMed] [Google Scholar]