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. 1986 Jul 1;237(1):259–263. doi: 10.1042/bj2370259

Glucose-induced accumulation of inositol trisphosphates in isolated pancreatic islets. Predominance of the 1,3,4-isomer.

J Turk, B A Wolf, M L McDaniel
PMCID: PMC1146973  PMID: 3541896

Abstract

Anion-exchange h.p.l.c. analysis of [3H]inositol phosphates derived from glucose-stimulated isolated pancreatic islets that had been prelabelled with myo-[3H]inositol revealed that the predominant inositol trisphosphate was the 1,3,4-isomer [Ins(1,3,4)P3]. The 1,4,5-isomer [Ins(1,4,5)P3] was also detectable, as was a more polar inositol phosphate with the chromatographic properties of inositol 1,3,4,5-tetrakisphosphate [Ins(1,3,4,5)P4]. Glucose-induced accumulation of Ins(1,3,4)P3 was augmented by Li+ and occurred after maximal accumulation of Ins(1,4,5)P3. These findings suggest a possible role for Ins(1,3,4)P3 or its probable precursor Ins(1,3,4,5)P4 in stimulus-secretion coupling in pancreatic islets.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Batty I. R., Nahorski S. R., Irvine R. F. Rapid formation of inositol 1,3,4,5-tetrakisphosphate following muscarinic receptor stimulation of rat cerebral cortical slices. Biochem J. 1985 Nov 15;232(1):211–215. doi: 10.1042/bj2320211. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berridge M. J., Irvine R. F. Inositol trisphosphate, a novel second messenger in cellular signal transduction. Nature. 1984 Nov 22;312(5992):315–321. doi: 10.1038/312315a0. [DOI] [PubMed] [Google Scholar]
  3. Berridge M. J. Rapid accumulation of inositol trisphosphate reveals that agonists hydrolyse polyphosphoinositides instead of phosphatidylinositol. Biochem J. 1983 Jun 15;212(3):849–858. doi: 10.1042/bj2120849. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Best L., Malaisse W. J. Nutrient and hormone-neurotransmitter stimuli induce hydrolysis of polyphosphoinositides in rat pancreatic islets. Endocrinology. 1984 Nov;115(5):1814–1820. doi: 10.1210/endo-115-5-1814. [DOI] [PubMed] [Google Scholar]
  5. Biden T. J., Prentki M., Irvine R. F., Berridge M. J., Wollheim C. B. Inositol 1,4,5-trisphosphate mobilizes intracellular Ca2+ from permeabilized insulin-secreting cells. Biochem J. 1984 Oct 15;223(2):467–473. doi: 10.1042/bj2230467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Burgess G. M., McKinney J. S., Irvine R. F., Putney J. W., Jr Inositol 1,4,5-trisphosphate and inositol 1,3,4-trisphosphate formation in Ca2+-mobilizing-hormone-activated cells. Biochem J. 1985 Nov 15;232(1):237–243. doi: 10.1042/bj2320237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Colca J. R., Wolf B. A., Comens P. G., McDaniel M. L. Protein phosphorylation in permeabilized pancreatic islet cells. Biochem J. 1985 Jun 15;228(3):529–536. doi: 10.1042/bj2280529. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Irvine R. F., Anggård E. E., Letcher A. J., Downes C. P. Metabolism of inositol 1,4,5-trisphosphate and inositol 1,3,4-trisphosphate in rat parotid glands. Biochem J. 1985 Jul 15;229(2):505–511. doi: 10.1042/bj2290505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Irvine R. F., Letcher A. J., Lander D. J., Downes C. P. Inositol trisphosphates in carbachol-stimulated rat parotid glands. Biochem J. 1984 Oct 1;223(1):237–243. doi: 10.1042/bj2230237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Joseph S. K., Williams R. J., Corkey B. E., Matschinsky F. M., Williamson J. R. The effect of inositol trisphosphate on Ca2+ fluxes in insulin-secreting tumor cells. J Biol Chem. 1984 Nov 10;259(21):12952–12955. [PubMed] [Google Scholar]
  11. Litosch I., Wallis C., Fain J. N. 5-Hydroxytryptamine stimulates inositol phosphate production in a cell-free system from blowfly salivary glands. Evidence for a role of GTP in coupling receptor activation to phosphoinositide breakdown. J Biol Chem. 1985 May 10;260(9):5464–5471. [PubMed] [Google Scholar]
  12. Martin T. F., Lucas D. O., Bajjalieh S. M., Kowalchyk J. A. Thyrotropin-releasing hormone activates a Ca2+-dependent polyphosphoinositide phosphodiesterase in permeable GH3 cells. GTP gamma S potentiation by a cholera and pertussis toxin-insensitive mechanism. J Biol Chem. 1986 Feb 25;261(6):2918–2927. [PubMed] [Google Scholar]
  13. Michell B. Inositol phosphates. Profusion and confusion. Nature. 1986 Jan 16;319(6050):176–177. doi: 10.1038/319176a0. [DOI] [PubMed] [Google Scholar]
  14. Montague W., Morgan N. G., Rumford G. M., Prince C. A. Effect of glucose on polyphosphoinositide metabolism in isolated rat islets of Langerhans. Biochem J. 1985 Apr 15;227(2):483–489. doi: 10.1042/bj2270483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Morgan N. G., Rumford G. M., Montague W. Studies on the role of inositol trisphosphate in the regulation of insulin secretion from isolated rat islets of Langerhans. Biochem J. 1985 Jun 15;228(3):713–718. doi: 10.1042/bj2280713. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Prentki M., Biden T. J., Janjic D., Irvine R. F., Berridge M. J., Wollheim C. B. Rapid mobilization of Ca2+ from rat insulinoma microsomes by inositol-1,4,5-trisphosphate. Nature. 1984 Jun 7;309(5968):562–564. doi: 10.1038/309562a0. [DOI] [PubMed] [Google Scholar]
  17. Rorsman P., Abrahamsson H., Gylfe E., Hellman B. Dual effects of glucose on the cytosolic Ca2+ activity of mouse pancreatic beta-cells. FEBS Lett. 1984 May 7;170(1):196–200. doi: 10.1016/0014-5793(84)81398-8. [DOI] [PubMed] [Google Scholar]
  18. Spät A., Bradford P. G., McKinney J. S., Rubin R. P., Putney J. W., Jr A saturable receptor for 32P-inositol-1,4,5-triphosphate in hepatocytes and neutrophils. Nature. 1986 Feb 6;319(6053):514–516. doi: 10.1038/319514a0. [DOI] [PubMed] [Google Scholar]
  19. Turk J., Colca J. R., Kotagal N., McDaniel M. L. Arachidonic acid metabolism in isolated pancreatic islets. I. Identification and quantitation of lipoxygenase and cyclooxygenase products. Biochim Biophys Acta. 1984 Jun 6;794(1):110–124. doi: 10.1016/0005-2760(84)90304-7. [DOI] [PubMed] [Google Scholar]
  20. Turk J., Colca J. R., Kotagal N., McDaniel M. L. Arachidonic acid metabolism in isolated pancreatic islets. II. The effects of glucose and of inhibitors of arachidonate metabolism on insulin secretion and metabolite synthesis. Biochim Biophys Acta. 1984 Jun 6;794(1):125–136. doi: 10.1016/0005-2760(84)90305-9. [DOI] [PubMed] [Google Scholar]
  21. Vergara J., Tsien R. Y., Delay M. Inositol 1,4,5-trisphosphate: a possible chemical link in excitation-contraction coupling in muscle. Proc Natl Acad Sci U S A. 1985 Sep;82(18):6352–6356. doi: 10.1073/pnas.82.18.6352. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wolf B. A., Comens P. G., Ackermann K. E., Sherman W. R., McDaniel M. L. The digitonin-permeabilized pancreatic islet model. Effect of myo-inositol 1,4,5-trisphosphate on Ca2+ mobilization. Biochem J. 1985 May 1;227(3):965–969. doi: 10.1042/bj2270965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wolf B. A., Turk J., Sherman W. R., McDaniel M. L. Intracellular Ca2+ mobilization by arachidonic acid. Comparison with myo-inositol 1,4,5-trisphosphate in isolated pancreatic islets. J Biol Chem. 1986 Mar 15;261(8):3501–3511. [PubMed] [Google Scholar]
  24. Wollheim C. B., Sharp G. W. Regulation of insulin release by calcium. Physiol Rev. 1981 Oct;61(4):914–973. doi: 10.1152/physrev.1981.61.4.914. [DOI] [PubMed] [Google Scholar]

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