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. 1986 Jul 15;237(2):597–600. doi: 10.1042/bj2370597

Induction of haem synthesis in Hep G2 human hepatoma cells by dimethyl sulphoxide. A transcriptionally activated event.

R A Galbraith, S Sassa, A Kappas
PMCID: PMC1147026  PMID: 3026326

Abstract

Exposure of cultured human hepatoma cells (Hep G2) to medium containing 2% (v/v) dimethyl sulphoxide resulted in an approximate doubling in the activity of delta-aminolaevulinate dehydratase, an increase in the haem content and a decreased growth rate; induced enzyme activity was decrease by 50% after treatment with alpha-amanitin. The findings are strikingly similar to those seen in murine Friend-virus-transformed erythroleukaemia cells.

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Selected References

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  1. Aikawa M., Schwartz A., Uni S., Nussenzweig R., Hollingdale M. Ultrastructure of in vitro cultured exoerythrocytic stage of Plasmodium berghei in a hepatoma cell line. Am J Trop Med Hyg. 1984 Sep;33(5):792–799. doi: 10.4269/ajtmh.1984.33.792. [DOI] [PubMed] [Google Scholar]
  2. Andersson L. C., Jokinen M., Gahmberg C. G. Induction of erythroid differentiation in the human leukaemia cell line K562. Nature. 1979 Mar 22;278(5702):364–365. doi: 10.1038/278364a0. [DOI] [PubMed] [Google Scholar]
  3. Bauer H. C., Parent J. B., Olden K. Role of carbohydrate in glycoprotein secretion by human hepatoma cells. Biochem Biophys Res Commun. 1985 Apr 16;128(1):368–375. doi: 10.1016/0006-291x(85)91688-2. [DOI] [PubMed] [Google Scholar]
  4. Chang C. S., Sassa S. Induction of delta-aminolevulinic acid dehydratase in mouse Friend virus-transformed erythroleukemia cells during erythroid differentiation. Blood. 1984 Jul;64(1):64–70. [PubMed] [Google Scholar]
  5. Cohen L. H., Griffioen M., Havekes L., Schouten D., van Hinsbergh V., Kempen H. J. Effects of compactin, mevalonate and low-density lipoprotein on 3-hydroxy-3-methylglutaryl-coenzyme A reductase activity and low-density-lipoprotein-receptor activity in the human hepatoma cell line Hep G2. Biochem J. 1984 Aug 15;222(1):35–39. doi: 10.1042/bj2220035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Collins S. J., Ruscetti F. W., Gallagher R. E., Gallo R. C. Terminal differentiation of human promyelocytic leukemia cells induced by dimethyl sulfoxide and other polar compounds. Proc Natl Acad Sci U S A. 1978 May;75(5):2458–2462. doi: 10.1073/pnas.75.5.2458. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dawson J. R., Adams D. J., Wolf C. R. Induction of drug metabolizing enzymes in human liver cell line Hep G2. FEBS Lett. 1985 Apr 22;183(2):219–222. doi: 10.1016/0014-5793(85)80780-8. [DOI] [PubMed] [Google Scholar]
  8. Dearfield K. L., Jacobson-Kram D., Brown N. A., Williams J. R. Evaluation of a human hepatoma cell line as a target cell in genetic toxicology. Mutat Res. 1983 Mar;108(1-3):437–449. doi: 10.1016/0027-5107(83)90138-0. [DOI] [PubMed] [Google Scholar]
  9. Diamond L., Kruszewski F., Aden D. P., Knowles B. B., Baird W. M. Metabolic activation of benzo[a]pyrene by a human hepatoma cell line. Carcinogenesis. 1980;1(10):871–875. doi: 10.1093/carcin/1.10.871. [DOI] [PubMed] [Google Scholar]
  10. Friend C., Scher W., Holland J. G., Sato T. Hemoglobin synthesis in murine virus-induced leukemic cells in vitro: stimulation of erythroid differentiation by dimethyl sulfoxide. Proc Natl Acad Sci U S A. 1971 Feb;68(2):378–382. doi: 10.1073/pnas.68.2.378. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gambari R., Marks P. A., Rifkind R. A. Murine erythroleukemia cell differentiation: relationship of globin gene expression and of prolongation of G1 to inducer effects during G1/early S. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4511–4515. doi: 10.1073/pnas.76.9.4511. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gordon J. I., Sims H. F., Edelstein C., Scanu A. M., Strauss A. W. Human proapolipoprotein A-II is cleaved following secretion from Hep G2 cells by a thiol protease. J Biol Chem. 1984 Dec 25;259(24):15556–15563. [PubMed] [Google Scholar]
  13. Guzelian P. S., O'Connor L., Fernandez S., Chan W., Giampietro P., Desnick R. Rapid loss of delta-aminolevulinic acid dehydratase activity in primary cultures of adult rat hepatocytes: a new model of zinc deficiency. Life Sci. 1982 Sep 13;31(11):1111–1116. doi: 10.1016/0024-3205(82)90084-4. [DOI] [PubMed] [Google Scholar]
  14. Hagihara B., Sato N., Fukuhara T., Tsutsumi K., Oyanagui Y. Spectrophotometric analysis of cytochromes in Morris hepatomas. Cancer Res. 1973 Nov;33(11):2947–2953. [PubMed] [Google Scholar]
  15. Haspel H. C., Birnbaum M. J., Wilk E. W., Rosen O. M. Biosynthetic precursors and in vitro translation products of the glucose transporter of human hepatocarcinoma cells, human fibroblasts, and murine preadipocytes. J Biol Chem. 1985 Jun 25;260(12):7219–7225. [PubMed] [Google Scholar]
  16. Higgins P. J., Borenfreund E. Enhanced albumin production by malignantly transformed hepatocytes during in vitro exposure to dimethylsulfoxide. Biochim Biophys Acta. 1980 Nov 14;610(1):174–180. doi: 10.1016/0005-2787(80)90067-2. [DOI] [PubMed] [Google Scholar]
  17. Hoffman R., Murnane M. J., Benz E. J., Jr, Prohaska R., Floyd V., Dainiak N., Forget B. G., Furthmayr H. Induction of erythropoietic colonies in a human chronic myelogenous leukemia cell line. Blood. 1979 Nov;54(5):1182–1187. [PubMed] [Google Scholar]
  18. Huberman E., Heckman C., Langenbach R. Stimulation of differentiated functions in human melanoma cells by tumor-promoting agents and dimethyl sulfoxide. Cancer Res. 1979 Jul;39(7 Pt 1):2618–2624. [PubMed] [Google Scholar]
  19. Knowles B. B., Howe C. C., Aden D. P. Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science. 1980 Jul 25;209(4455):497–499. doi: 10.1126/science.6248960. [DOI] [PubMed] [Google Scholar]
  20. Marks P. A., Rifkind R. A. Erythroleukemic differentiation. Annu Rev Biochem. 1978;47:419–448. doi: 10.1146/annurev.bi.47.070178.002223. [DOI] [PubMed] [Google Scholar]
  21. McIntyre L. J., Kim Y. S. Effects of sodium butyrate and dimethylsulfoxide on human pancreatic tumor cell lines. Eur J Cancer Clin Oncol. 1984 Feb;20(2):265–271. doi: 10.1016/0277-5379(84)90194-9. [DOI] [PubMed] [Google Scholar]
  22. Murata Y., Sarne D. H., Horwitz A. L., Lecocq R., Aden D. P., Knowles B. B., Refetoff S. Characterization of thyroxine-binding globulin secreted by a human hepatoma cell line. J Clin Endocrinol Metab. 1985 Mar;60(3):472–478. doi: 10.1210/jcem-60-3-472. [DOI] [PubMed] [Google Scholar]
  23. Póvoa G., Isaksson M., Jörnvall H., Hall K. The somatomedin-binding protein isolated from a human hepatoma cell line is identical to the human amniotic fluid somatomedin-binding protein. Biochem Biophys Res Commun. 1985 May 16;128(3):1071–1078. doi: 10.1016/0006-291x(85)91049-6. [DOI] [PubMed] [Google Scholar]
  24. Rosner W., Aden D. P., Khan M. S. Hormonal influences on the secretion of steroid-binding proteins by a human hepatoma-derived cell line. J Clin Endocrinol Metab. 1984 Oct;59(4):806–808. doi: 10.1210/jcem-59-4-806. [DOI] [PubMed] [Google Scholar]
  25. Sassa S. Delta-aminolevulinic acid dehydratase assay. Enzyme. 1982;28(2-3):133–145. doi: 10.1159/000459097. [DOI] [PubMed] [Google Scholar]
  26. Sassa S., Kappas A. Induction of aminolevulinate synthase and porphyrins in cultured liver cells maintained in chemically defined medium. Permissive effects of hormones on induction process. J Biol Chem. 1977 Apr 10;252(7):2428–2436. [PubMed] [Google Scholar]
  27. Sassa S. Sequential induction of heme pathway enzymes during erythroid differentiation of mouse Friend leukemia virus-infected cells. J Exp Med. 1976 Feb 1;143(2):305–315. doi: 10.1084/jem.143.2.305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Sugimura T., Ikeda K., Hirota K., Hozumi M., Morris H. P. Chemical, enzymatic, and cytochrome assays of microsomal fraction of hepatomas with different growth rates. Cancer Res. 1966 Aug;26(8):1711–1716. [PubMed] [Google Scholar]
  29. Swaneck G. E., Nordstrom J. L., Kreuzaler F., Tsai M. J., O'Malley B. W. Effect of estrogen on gene expression in chicken oviduct: evidence for transcriptional control of ovalbumin gene. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1049–1053. doi: 10.1073/pnas.76.3.1049. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Verspohl E. J., Roth R. A., Vigneri R., Goldfine I. D. Dual regulation of glycogen metabolism by insulin and insulin-like growth factors in human hepatoma cells (HEP-G2). Analysis with an anti-receptor monoclonal antibody. J Clin Invest. 1984 Oct;74(4):1436–1443. doi: 10.1172/JCI111555. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Wilson D. B., Salem H. H., Mruk J. S., Maruyama I., Majerus P. W. Biosynthesis of coagulation Factor V by a human hepatocellular carcinoma cell line. J Clin Invest. 1984 Mar;73(3):654–658. doi: 10.1172/JCI111256. [DOI] [PMC free article] [PubMed] [Google Scholar]

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