Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1986 Jul 15;237(2):601–604. doi: 10.1042/bj2370601

Regulation of superoxide generation by myeloperoxidase during the respiratory burst of human neutrophils.

S W Edwards, T F Swan
PMCID: PMC1147027  PMID: 3026327

Abstract

The role of myeloperoxidase in the regulation of the respiratory burst of human neutrophils activated by the chemotactic peptide (N-formyl-L-methionyl-L-leucyl-L-phenylalanine) plus cytochalasin B was determined by using anti-(human myeloperoxidase) antibody. The respiratory burst activated under these conditions consisted of an initial (1-2 min) phase with high rates of O2 uptake, luminol-dependent chemiluminescence and superoxide radical (O2-.) generation and a second, more sustained, phase of lower magnitude of chemiluminescence and O2 uptake: O2-. generation did not occur during this second phase. In cell suspensions stimulated in the presence of anti-(human myeloperoxidase) antibody, the magnitude of the initial phase of both O2 uptake and O2-. generation was unaffected, but these high rates were maintained over much longer periods than in control suspensions. It is therefore proposed that a product of myeloperoxidase normally regulates the duration of O2-. generation during the respiratory burst, possibly by inhibition of NADPH oxidase.

Full text

PDF
601

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Babior B. M., Kipnes R. S., Curnutte J. T. Biological defense mechanisms. The production by leukocytes of superoxide, a potential bactericidal agent. J Clin Invest. 1973 Mar;52(3):741–744. doi: 10.1172/JCI107236. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Babior B. M. Oxygen-dependent microbial killing by phagocytes (first of two parts). N Engl J Med. 1978 Mar 23;298(12):659–668. doi: 10.1056/NEJM197803232981205. [DOI] [PubMed] [Google Scholar]
  3. Bellavite P., Jones O. T., Cross A. R., Papini E., Rossi F. Composition of partially purified NADPH oxidase from pig neutrophils. Biochem J. 1984 Nov 1;223(3):639–648. doi: 10.1042/bj2230639. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cech P., Papathanassiou A., Boreux G., Roth P., Miescher P. A. Hereditary myeloperoxidase deficiency. Blood. 1979 Mar;53(3):403–411. [PubMed] [Google Scholar]
  5. Cross A. R., Parkinson J. F., Jones O. T. The superoxide-generating oxidase of leucocytes. NADPH-dependent reduction of flavin and cytochrome b in solubilized preparations. Biochem J. 1984 Oct 15;223(2):337–344. doi: 10.1042/bj2230337. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dri P., Soranzo M. R., Cramer R., Menegazzi R., Miotti V., Patriarca P. Role of myeloperoxidase in respiratory burst of human polymorphonuclear leukocytes. Studies with myeloperoxidase-deficient subjects. Inflammation. 1985 Mar;9(1):21–31. doi: 10.1007/BF00915408. [DOI] [PubMed] [Google Scholar]
  7. Edwards S. W., Hallett M. B., Lloyd D., Campbell A. K. Decrease in apparent Km for oxygen after stimulation of respiration of rat polymorphonuclear leukocytes. FEBS Lett. 1983 Sep 5;161(1):60–64. doi: 10.1016/0014-5793(83)80730-3. [DOI] [PubMed] [Google Scholar]
  8. Goldstein I. M. Neutrophil degranulation. Contemp Top Immunobiol. 1984;14:189–219. doi: 10.1007/978-1-4757-4862-8_7. [DOI] [PubMed] [Google Scholar]
  9. Gutteridge J. M., Rowley D. A., Halliwell B. Superoxide-dependent formation of hydroxyl radicals in the presence of iron salts. Detection of 'free' iron in biological systems by using bleomycin-dependent degradation of DNA. Biochem J. 1981 Oct 1;199(1):263–265. doi: 10.1042/bj1990263. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Halliwell B., Gutteridge J. M. Oxygen toxicity, oxygen radicals, transition metals and disease. Biochem J. 1984 Apr 1;219(1):1–14. doi: 10.1042/bj2190001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Harrison J. E., Schultz J. Studies on the chlorinating activity of myeloperoxidase. J Biol Chem. 1976 Mar 10;251(5):1371–1374. [PubMed] [Google Scholar]
  12. Jandl R. C., André-Schwartz J., Borges-DuBois L., Kipnes R. S., McMurrich B. J., Babior B. M. Termination of the respiratory burst in human neutrophils. J Clin Invest. 1978 May;61(5):1176–1185. doi: 10.1172/JCI109033. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lutter R., van Zwieten R., Weening R. S., Hamers M. N., Roos D. Cytochrome b, flavins, and ubiquinone-50 in enucleated human neutrophils (polymorphonuclear leukocyte cytoplasts). J Biol Chem. 1984 Aug 10;259(15):9603–9606. [PubMed] [Google Scholar]
  14. Morel F., Doussiere J., Stasia M. J., Vignais P. V. The respiratory burst of bovine neutrophils. Role of a b type cytochrome and coenzyme specificity. Eur J Biochem. 1985 Nov 4;152(3):669–679. doi: 10.1111/j.1432-1033.1985.tb09247.x. [DOI] [PubMed] [Google Scholar]
  15. Nauseef W. M., Metcalf J. A., Root R. K. Role of myeloperoxidase in the respiratory burst of human neutrophils. Blood. 1983 Mar;61(3):483–492. [PubMed] [Google Scholar]
  16. Ohno Y., Seligmann B. E., Gallin J. I. Cytochrome b translocation to human neutrophil plasma membranes and superoxide release. Differential effects of N-formylmethionylleucylphenylalanine, phorbol myristate acetate, and A23187. J Biol Chem. 1985 Feb 25;260(4):2409–2414. [PubMed] [Google Scholar]
  17. Pember S. O., Shapira R., Kinkade J. M., Jr Multiple forms of myeloperoxidase from human neutrophilic granulocytes: evidence for differences in compartmentalization, enzymatic activity, and subunit structure. Arch Biochem Biophys. 1983 Mar;221(2):391–403. doi: 10.1016/0003-9861(83)90158-3. [DOI] [PubMed] [Google Scholar]
  18. Rosen H., Klebanoff S. J. Chemiluminescence and superoxide production by myeloperoxidase-deficient leukocytes. J Clin Invest. 1976 Jul;58(1):50–60. doi: 10.1172/JCI108458. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sagone A. L., Jr, Mendelson D. S., Metz E. N. The effect of sodium azide on the chemiluminescence of granulocytes--evidence for the generation of multiple oxygen radicals. J Lab Clin Med. 1977 Jun;89(6):1333–1340. [PubMed] [Google Scholar]
  20. Stelmaszyńska T., Zgliczyński J. M. Myeloperoxidase of human neutrophilic granulocytes as chlorinating enzyme. Eur J Biochem. 1974 Jun 1;45(1):305–312. doi: 10.1111/j.1432-1033.1974.tb03555.x. [DOI] [PubMed] [Google Scholar]
  21. Winterbourn C. C. Comparative reactivities of various biological compounds with myeloperoxidase-hydrogen peroxide-chloride, and similarity of the oxidant to hypochlorite. Biochim Biophys Acta. 1985 Jun 18;840(2):204–210. doi: 10.1016/0304-4165(85)90120-5. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES