Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1986 Sep 15;238(3):677–682. doi: 10.1042/bj2380677

Rapid purification of tissue inhibitor of metalloproteinases from human plasma and identification as a gamma-serum protein.

T E Cawston, D N Noble, G Murphy, A J Smith, C Woodley, B Hazleman
PMCID: PMC1147191  PMID: 3099772

Abstract

A rapid method is described for the purification of human tissue inhibitor of metalloproteinases (TIMP) from plasma which involves immuno-affinity chromatography and gel filtration. The purified plasma inhibitor is immunologically identical with the TIMP previously purified from human amniotic fluid, human synovial fluid and human fibroblast culture medium. It is proposed that this inhibitor is identical with the plasma inhibitor previously named 'B1 anticollagenase', although the plasma inhibitor was shown to migrate as a gamma-serum component.

Full text

PDF
677

Images in this article

679Fig. 3.
on p.679
680Fig. 5.
on p.680
681Fig. 6.
on p.681

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bunning R. A., Murphy G., Kumar S., Phillips P., Reynolds J. J. Metalloproteinase inhibitors from bovine cartilage and body fluids. Eur J Biochem. 1984 Feb 15;139(1):75–80. doi: 10.1111/j.1432-1033.1984.tb07978.x. [DOI] [PubMed] [Google Scholar]
  2. Cawston T. E., Barrett A. J. A rapid and reproducible assay for collagenase using [1-14C]acetylated collagen. Anal Biochem. 1979 Nov 1;99(2):340–345. doi: 10.1016/s0003-2697(79)80017-2. [DOI] [PubMed] [Google Scholar]
  3. Cawston T. E., Galloway W. A., Mercer E., Murphy G., Reynolds J. J. Purification of rabbit bone inhibitor of collagenase. Biochem J. 1981 Apr 1;195(1):159–165. doi: 10.1042/bj1950159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cawston T. E., Murphy G., Mercer E., Galloway W. A., Hazleman B. L., Reynolds J. J. The interaction of purified rabbit bone collagenase with purified rabbit bone metalloproteinase inhibitor. Biochem J. 1983 May 1;211(2):313–318. doi: 10.1042/bj2110313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cooper T. W., Eisen A. Z., Stricklin G. P., Welgus H. G. Platelet-derived collagenase inhibitor: characterization and subcellular localization. Proc Natl Acad Sci U S A. 1985 May;82(9):2779–2783. doi: 10.1073/pnas.82.9.2779. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Docherty A. J., Lyons A., Smith B. J., Wright E. M., Stephens P. E., Harris T. J., Murphy G., Reynolds J. J. Sequence of human tissue inhibitor of metalloproteinases and its identity to erythroid-potentiating activity. Nature. 1985 Nov 7;318(6041):66–69. doi: 10.1038/318066a0. [DOI] [PubMed] [Google Scholar]
  7. Hembry R. M., Murphy G., Reynolds J. J. Immunolocalization of tissue inhibitor of metalloproteinases (TIMP) in human cells. Characterization and use of a specific antiserum. J Cell Sci. 1985 Feb;73:105–119. doi: 10.1242/jcs.73.1.105. [DOI] [PubMed] [Google Scholar]
  8. Macartney H. W., Tschesche H. Characterisation of beta 1-anticollagenase from human plasma and its reaction with polymorphonuclear leukocyte collagenase by disulfide/thiol interchange. Eur J Biochem. 1983 Jan 17;130(1):85–92. doi: 10.1111/j.1432-1033.1983.tb07120.x. [DOI] [PubMed] [Google Scholar]
  9. Macartney H. W., Tschesche H. Interaction of beta 1-anticollagenase from human plasma with collagenases from various tissues and competition with alpha 2-macroglobulin. Eur J Biochem. 1983 Jan 17;130(1):93–97. doi: 10.1111/j.1432-1033.1983.tb07121.x. [DOI] [PubMed] [Google Scholar]
  10. Macartney H. W., Tschesche H. The collagenase inhibitor from human polymorphonuclear leukocytes. Isolation, purification and characterisation. Eur J Biochem. 1983 Jan 17;130(1):79–83. doi: 10.1111/j.1432-1033.1983.tb07119.x. [DOI] [PubMed] [Google Scholar]
  11. Mercer E., Cawston T. E., de Silva M., Hazleman B. L. Purification of a metalloproteinase inhibitor from human rheumatoid synovial fluid. Biochem J. 1985 Nov 1;231(3):505–510. doi: 10.1042/bj2310505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Murphy G., Cartwright E. C., Sellers A., Reynolds J. J. The detection and characterisation of collagenase inhibitors from rabbit tissues in culture. Biochim Biophys Acta. 1977 Aug 11;483(2):493–498. doi: 10.1016/0005-2744(77)90080-8. [DOI] [PubMed] [Google Scholar]
  13. Murphy G., Cawston T. E., Galloway W. A., Barnes M. J., Bunning R. A., Mercer E., Reynolds J. J., Burgeson R. E. Metalloproteinases from rabbit bone culture medium degrade types IV and V collagens, laminin and fibronectin. Biochem J. 1981 Dec 1;199(3):807–811. doi: 10.1042/bj1990807. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Murphy G., Cawston T. E., Reynolds J. J. An inhibitor of collagenase from human amniotic fluid. Purification, characterization and action on metalloproteinases. Biochem J. 1981 Apr 1;195(1):167–170. doi: 10.1042/bj1950167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Murphy G., Reynolds J. J., Werb Z. Biosynthesis of tissue inhibitor of metalloproteinases by human fibroblasts in culture. Stimulation by 12-O-tetradecanoylphorbol 13-acetate and interleukin 1 in parallel with collagenase. J Biol Chem. 1985 Mar 10;260(5):3079–3083. [PubMed] [Google Scholar]
  16. Stricklin G. P., Welgus H. G. Human skin fibroblast collagenase inhibitor. Purification and biochemical characterization. J Biol Chem. 1983 Oct 25;258(20):12252–12258. [PubMed] [Google Scholar]
  17. Vater C. A., Mainardi C. L., Harris E. D., Jr Inhibitor of human collagenase from cultures of human tendon. J Biol Chem. 1979 Apr 25;254(8):3045–3053. [PubMed] [Google Scholar]
  18. Welgus H. G., Stricklin G. P. Human skin fibroblast collagenase inhibitor. Comparative studies in human connective tissues, serum, and amniotic fluid. J Biol Chem. 1983 Oct 25;258(20):12259–12264. [PubMed] [Google Scholar]
  19. Woolley D. E., Roberts D. R., Evanson J. M. Small molecular weight beta 1 serum protein which specifically inhibits human collagenases. Nature. 1976 May 27;261(5558):325–327. doi: 10.1038/261325a0. [DOI] [PubMed] [Google Scholar]
  20. Yasui N., Hori H., Nagai Y. Production of collagenase inhibitor by the growth cartilage of embryonic chick bone: isolation and partial characterization. Coll Relat Res. 1981;1(1):59–72. doi: 10.1016/s0174-173x(80)80008-2. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES