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. 1986 Sep 15;238(3):919–922. doi: 10.1042/bj2380919

Purification and subunit structure of phosphoglycerate dehydrogenase from rabbit liver.

K Lund, D K Merrill, R W Guynn
PMCID: PMC1147222  PMID: 3800969

Abstract

D-3-Phosphoglycerate dehydrogenase (EC 1.1.1.95) was purified from rabbit liver by (NH4)2SO4 fractionation, DEAE-Sephacel chromatography, affinity chromatography on AMP-agarose and molecular-sieve h.p.l.c. The purified enzyme was homogeneous as judged by SDS/polyacrylamide-slab-gel electrophoresis. On the basis of molecular-sieve h.p.l.c. and SDS/polyacrylamide-gel electrophoresis, the enzyme is a tetramer composed of subunits of Mr 60,000.

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Selected References

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