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. 1986 Oct 15;239(2):263–267. doi: 10.1042/bj2390263

Urea synthesis in freshly isolated and in cultured periportal and perivenous hepatocytes.

A R Pösö, K E Penttilä, E M Suolinna, K O Lindros
PMCID: PMC1147276  PMID: 3814074

Abstract

Periportal hepatocytes isolated by digitonin/collagenase perfusion produced urea faster than did similarly prepared perivenous hepatocytes, in both the presence and the absence of amino acids and various urea precursors. There was no difference between the two cell types in rates of intracellular proteolysis. The initial difference in urea synthesis persisted for 5 days during primary culture, but then gradually disappeared. Our results demonstrate that the periportal dominance of urea formation is unrelated to the currently existing acinar microenvironment in the intact liver, but probably reflects differences in acinar key enzyme activities only slowly converging during culture.

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Selected References

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  1. Bengtsson B. G., Kiessling K. H., Smith-Kielland A., Mørland J. Partial separation and biochemical characteristics of periportal and perivenous hepatocytes from rat liver. Eur J Biochem. 1981 Sep 1;118(3):591–597. doi: 10.1111/j.1432-1033.1981.tb05560.x. [DOI] [PubMed] [Google Scholar]
  2. Berry M. N., Friend D. S. High-yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study. J Cell Biol. 1969 Dec;43(3):506–520. doi: 10.1083/jcb.43.3.506. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Deuel T. F., Louie M., Lerner A. Glutamine synthetase from rat liver. Purification, properties, and preparation of specific antisera. J Biol Chem. 1978 Sep 10;253(17):6111–6118. [PubMed] [Google Scholar]
  4. Gebhardt R., Mecke D. Heterogeneous distribution of glutamine synthetase among rat liver parenchymal cells in situ and in primary culture. EMBO J. 1983;2(4):567–570. doi: 10.1002/j.1460-2075.1983.tb01464.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Grinde B., Seglen P. O. Effects of amino acid analogues on protein degradation in isolated rat hepatocytes. Biochim Biophys Acta. 1981 Aug 5;676(1):43–50. doi: 10.1016/0304-4165(81)90007-6. [DOI] [PubMed] [Google Scholar]
  6. Hensgens H. E., Meijer A. J. The interrelationship between ureogenesis and protein synthesis in isolated rat-liver cells. Biochim Biophys Acta. 1979 Feb 1;582(3):525–532. doi: 10.1016/0304-4165(79)90143-0. [DOI] [PubMed] [Google Scholar]
  7. Häussinger D. Hepatocyte heterogeneity in glutamine and ammonia metabolism and the role of an intercellular glutamine cycle during ureogenesis in perfused rat liver. Eur J Biochem. 1983 Jun 15;133(2):269–275. doi: 10.1111/j.1432-1033.1983.tb07458.x. [DOI] [PubMed] [Google Scholar]
  8. Jungermann K., Katz N. Functional hepatocellular heterogeneity. Hepatology. 1982 May-Jun;2(3):385–395. doi: 10.1002/hep.1840020316. [DOI] [PubMed] [Google Scholar]
  9. Lin R. C., Snodgrass P. J. Primary culture of normal adult rat liver cells which maintain stable urea cycle enzymes. Biochem Biophys Res Commun. 1975 May 19;64(2):725–734. doi: 10.1016/0006-291x(75)90380-0. [DOI] [PubMed] [Google Scholar]
  10. Lindros K. O., Penttilä K. E. Digitonin-collagenase perfusion for efficient separation of periportal or perivenous hepatocytes. Biochem J. 1985 Jun 15;228(3):757–760. doi: 10.1042/bj2280757. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lusty C. J. Carbamoylphosphate synthetase I of rat-liver mitochondria. Purification, properties, and polypeptide molecular weight. Eur J Biochem. 1978 Apr 17;85(2):373–383. doi: 10.1111/j.1432-1033.1978.tb12249.x. [DOI] [PubMed] [Google Scholar]
  12. Meijer A. J., Lof C., Ramos I. C., Verhoeven A. J. Control of ureogenesis. Eur J Biochem. 1985 Apr 1;148(1):189–196. doi: 10.1111/j.1432-1033.1985.tb08824.x. [DOI] [PubMed] [Google Scholar]
  13. Mizutani A. Cytochemical demonstration of ornithine carbamoyltransferase activity in liver mitochondria of rat and mouse. J Histochem Cytochem. 1968 Mar;16(3):172–180. doi: 10.1177/16.3.172. [DOI] [PubMed] [Google Scholar]
  14. Quistorff B. Gluconeogenesis in periportal and perivenous hepatocytes of rat liver, isolated by a new high-yield digitonin/collagenase perfusion technique. Biochem J. 1985 Jul 1;229(1):221–226. doi: 10.1042/bj2290221. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Quistorff B., Grunnet N., Cornell N. W. Digitonin perfusion of rat liver. A new approach in the study of intra-acinar and intracellular compartmentation in the liver. Biochem J. 1985 Feb 15;226(1):289–297. doi: 10.1042/bj2260289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Richards G. M. Modifications of the diphenylamine reaction giving increased sensitivity and simplicity in the estimation of DNA. Anal Biochem. 1974 Feb;57(2):369–376. doi: 10.1016/0003-2697(74)90091-8. [DOI] [PubMed] [Google Scholar]
  17. Schworer C. M., Shiffer K. A., Mortimore G. E. Quantitative relationship between autophagy and proteolysis during graded amino acid deprivation in perfused rat liver. J Biol Chem. 1981 Jul 25;256(14):7652–7658. [PubMed] [Google Scholar]
  18. Seglen P. O. Effects of amino acids, ammonia and leupeptin on protein synthesis and degradation in isolated rat hepatocytes. Biochem J. 1978 Aug 15;174(2):469–474. doi: 10.1042/bj1740469. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Seglen P. O. Preparation of isolated rat liver cells. Methods Cell Biol. 1976;13:29–83. doi: 10.1016/s0091-679x(08)61797-5. [DOI] [PubMed] [Google Scholar]
  20. Seglen P. O. Protein-catabolic stage of isolated rat hepatocytes. Biochim Biophys Acta. 1977 Jan 24;496(1):182–191. doi: 10.1016/0304-4165(77)90126-x. [DOI] [PubMed] [Google Scholar]
  21. Smith-Kielland A., Bengtsson G., Svendsen L., Mørland J. Protein synthesis in different populations of rat hepatocytes separated according to density. J Cell Physiol. 1982 Mar;110(3):262–266. doi: 10.1002/jcp.1041100307. [DOI] [PubMed] [Google Scholar]
  22. Tanaka K., Sato M., Tomita Y., Ichihara A. Biochemical studies on liver functions in primary cultured hepatocytes of adult rats. I. Hormonal effects on cell viability and protein synthesis. J Biochem. 1978 Oct;84(4):937–946. doi: 10.1093/oxfordjournals.jbchem.a132207. [DOI] [PubMed] [Google Scholar]
  23. Turner A. J., Whittle S. R. Functions of aldehyde reductases. Biochem Soc Trans. 1981 Aug;9(4):279–281. doi: 10.1042/bst0090279. [DOI] [PubMed] [Google Scholar]
  24. Vänänen H., Lindros K. O., Salaspuro M. Selective isolation of intact periportal or perivenous hepatocytes by antero- or retrograde collagenase gradient perfusion. Liver. 1983 Jun;3(3):131–139. doi: 10.1111/j.1600-0676.1983.tb00861.x. [DOI] [PubMed] [Google Scholar]
  25. Wanders R. J., Hoek J. B., Tager J. M. Origin of the ammonia found in protein-free extracts of rat-liver mitochondria and rat hepatocytes. Eur J Biochem. 1980 Sep;110(1):197–202. doi: 10.1111/j.1432-1033.1980.tb04855.x. [DOI] [PubMed] [Google Scholar]
  26. Welsh F. A. Changes in distribution of enzymes within the liver lobule during adaptive increases. J Histochem Cytochem. 1972 Feb;20(2):107–111. doi: 10.1177/20.2.107. [DOI] [PubMed] [Google Scholar]
  27. Wojtczak A. B., Wałajtys-Rode E. I., Geelen M. J. Interrelations between ureogenesis and gluconeogenesis in isolated hepatocytes. The role of antion transport and the competition for energy. Biochem J. 1978 Feb 15;170(2):379–385. doi: 10.1042/bj1700379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Woodside K. H., Mortimore G. E. Suppression of protein turnover by amino acids in the perfused rat liver. J Biol Chem. 1972 Oct 25;247(20):6474–6481. [PubMed] [Google Scholar]

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