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. 1987 Mar 15;242(3):661–665. doi: 10.1042/bj2420661

Molecular cloning and over-expression of the glyoxylate bypass operon from Escherichia coli ML308.

E M el-Mansi, C MacKintosh, K Duncan, W H Holms, H G Nimmo
PMCID: PMC1147762  PMID: 3297049

Abstract

A recombinant plasmid carrying an 11 kb restriction-endonuclease-ClaI fragment of genomic DNA from Escherichia coli ML308 was constructed. This plasmid complements an aceA mutation. The plasmid encodes the structural genes of the glyoxylate bypass operon, namely malate synthase A (aceB), isocitrate lyase (aceA) and isocitrate dehydrogenase kinase/phosphatase (aceK), as judged by overexpression of enzyme activities and transcription/translation experiments in vitro. Subcloning confirmed that expression of the aceK gene is essential for growth on acetate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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