Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1987 Apr 1;243(1):75–78. doi: 10.1042/bj2430075

The effect of disodium ethane-1-hydroxy-1,1-diphosphonate on the metabolism of calcitriol in chicks.

C Lidor, M S Meyer, R H Wasserman, S Edelstein
PMCID: PMC1147816  PMID: 3038087

Abstract

Decreased intestinal absorption of Ca2+ occurs in response to treatment with disodium ethane-1-hydroxy-1,1-diphosphonate (EHDP). The effect is due to decreased 1-hydroxylation of calcidiol (25-hydroxycholecalciferol) in the kidney. In an attempt to establish whether impairment of vitamin D metabolism at steps beyond kidney hydroxylation occurs due to treatment with EHDP, chicks were depleted of vitamin D and were treated with calcitriol (1,25-dihydroxycholecalciferol) as their sole source of the vitamin. The chicks were then divided into two groups, one being treated with EHDP while the second group served as control. Intestinal absorption of Ca2+ in the EHDP-treated group was found to be impaired, along with decreases in concentrations of calbindin D28K (the 28,000-Mr vitamin D-dependent Ca2+-binding protein). When the chicks were dosed with [3H]calcitriol, significantly lower concentrations of the sterol were detected in the duodena of EHDP-treated birds. Measurement of levels of receptors for calcitriol in duodena showed no difference between groups, but levels of calcitriol in sera were considerably lower in the EHDP-treated group along with the elevated biliary and urinary excretion of glucuronidated conjugates. It is therefore concluded that treatment with EHDP results in increased catabolism of calcitriol in addition to the known suppression of the renal production of the hormone.

Full text

PDF
75

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  2. Bar A., Hurwitz S. Bone ash and duodenal calcium-binding protein in chicks treated with EHDP. Poult Sci. 1973 Nov;52(6):2338–2339. doi: 10.3382/ps.0522338. [DOI] [PubMed] [Google Scholar]
  3. Baxter L. A., Deluca H. F., Bonjour J. P., Fleisch H. A. Inhibition of vitamin D metabolism by ethane-1-hydroxyl-1, 1-diphosphonate. Arch Biochem Biophys. 1974 Oct;164(2):655–662. doi: 10.1016/0003-9861(74)90077-0. [DOI] [PubMed] [Google Scholar]
  4. Corradino R. A., Wasserman R. H. Vitamin D3: induction of calcium-binding protein in embryonic chick intestine in vitro. Science. 1971 May 14;172(3984):731–733. doi: 10.1126/science.172.3984.731. [DOI] [PubMed] [Google Scholar]
  5. Fleisch H., Russell R. G., Francis M. D. Diphosphonates inhibit hydroxyapatite dissolution in vitro and bone resorption in tissue culture and in vivo. Science. 1969 Sep 19;165(3899):1262–1264. doi: 10.1126/science.165.3899.1262. [DOI] [PubMed] [Google Scholar]
  6. Francis M. D., Russell R. G., Fleisch H. Diphosphonates inhibit formation of calcium phosphate crystals in vitro and pathological calcification in vivo. Science. 1969 Sep 19;165(3899):1264–1266. doi: 10.1126/science.165.3899.1264. [DOI] [PubMed] [Google Scholar]
  7. Gasser A. B., Morgan D. B., Fleisch H. A., Richelle L. J. The influence of two diphosphonates on calcium metabolism in the rat. Clin Sci. 1972 Jul;43(1):31–45. doi: 10.1042/cs0430031. [DOI] [PubMed] [Google Scholar]
  8. Hill L. F., Lumb G. A., Mawer E. B., Stanbury S. W. Indirect inhibition of the biosynthesis of 1,25-dihydroxycholecalciferol in rats treated with a diphosphonate. Clin Sci. 1973 Apr;44(4):335–347. doi: 10.1042/cs0440335. [DOI] [PubMed] [Google Scholar]
  9. Hurwitz S., Bar A. Site of vitamin D action in chick intestine. Am J Physiol. 1972 Mar;222(3):761–767. doi: 10.1152/ajplegacy.1972.222.3.761. [DOI] [PubMed] [Google Scholar]
  10. Morgan D. B., Bonjour J. P., Gasser A. B., O'Brien K., Fleisch H. A. The influence of a diphosphonate on the intestinal absorption of calcium. Isr J Med Sci. 1971 Mar;7(3):384–386. [PubMed] [Google Scholar]
  11. Morrissey R. L., Wasserman R. H. Calcium absorption and calcium-binding protein in chicks on differing calcium and phosphorus intakes. Am J Physiol. 1971 May;220(5):1509–1515. doi: 10.1152/ajplegacy.1971.220.5.1509. [DOI] [PubMed] [Google Scholar]
  12. Norman A. W., Bayless J. D., Tsai H. C. Biologic effects of short-term phenobarbital treatment on the response to vitamin D and its metabolites in the chick. Biochem Pharmacol. 1976 Jan 15;25(2):163–168. doi: 10.1016/0006-2952(76)90285-9. [DOI] [PubMed] [Google Scholar]
  13. Norman A. W., Hunziker W., Walters M. R., Bishop J. E. Differential effects of protease inhibitors on 1,25-dihydroxyvitamin D3 receptors. J Biol Chem. 1983 Nov 10;258(21):12876–12880. [PubMed] [Google Scholar]
  14. Taylor C. M., Mawer E. B., Reeve A. The effects of a diphosphonate and dietary calcium on the metabolism of vitamin D3 (cholecalciferol) in the chick. Clin Sci Mol Med. 1975 Nov;49(5):391–400. doi: 10.1042/cs0490391. [DOI] [PubMed] [Google Scholar]
  15. Trechsel U., Bonjour J. P., Fleisch H. Regulation of the metabolism of 25-hydroxyvitamin D3 in primary cultures of chick kidney cells. J Clin Invest. 1979 Jul;64(1):206–217. doi: 10.1172/JCI109441. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Trechsel U., Schenk R., Bonjour J. P., Russell R. G., Fleisch H. Relation between bone mineralization, Ca absorption, and plasma Ca in phosphonate-treated rats. Am J Physiol. 1977 Mar;232(3):E298–E305. doi: 10.1152/ajpendo.1977.232.3.E298. [DOI] [PubMed] [Google Scholar]
  17. Trechsel U., Taylor C. M., Eisman J. A., Bonjour J. P., Fleisch H. Plasma levels of vitamin D metabolites in diphosphonate-treated rats. Clin Sci (Lond) 1981 Oct;61(4):471–476. doi: 10.1042/cs0610471. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES