Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1987 Apr 1;243(1):281–284. doi: 10.1042/bj2430281

Direct assessment of brown adipose tissue as a site of systemic tri-iodothyronine production in the rat.

J A Fernandez, T Mampel, F Villarroya, R Iglesias
PMCID: PMC1147845  PMID: 3606577

Abstract

Tri-iodothyronine (T3)production by interscapular brown fat was studied by measurements of arterio-venous differences and blood flow across the tissue in rats exposed to the following situations: controls, acute cold, chronic cold and starvation. Results demonstrate that brown adipose tissue is a source of systemic T3 in the rat and that the T3 release is modulated according to the physiological situation of the animal: increased in cold exposure and inhibited in starvation.

Full text

PDF
281

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Desautels M. Mitochondrial thermogenin content is unchanged during atrophy of BAT of fasting mice. Am J Physiol. 1985 Jul;249(1 Pt 1):E99–106. doi: 10.1152/ajpendo.1985.249.1.E99. [DOI] [PubMed] [Google Scholar]
  2. Foster D. O., Depocas F., Frydman M. L. Noradrenaline-induced calorigenesis in warm- and cold-acclimated rats: relations between concentration of noradrenaline in arterial plasma, blood flow to differently located masses of brown adipose tissue, and calorigenic response. Can J Physiol Pharmacol. 1980 Aug;58(8):915–924. doi: 10.1139/y80-140. [DOI] [PubMed] [Google Scholar]
  3. Foster D. O., Frydman M. L. Tissue distribution of cold-induced thermogenesis in conscious warm- or cold-acclimated rats reevaluated from changes in tissue blood flow: the dominant role of brown adipose tissue in the replacement of shivering by nonshivering thermogenesis. Can J Physiol Pharmacol. 1979 Mar;57(3):257–270. doi: 10.1139/y79-039. [DOI] [PubMed] [Google Scholar]
  4. Giralt M., Villarroya F., Mampel T., Iglesias R. Impaired basal and noradrenaline-induced iodothyronine 5'-deiodinase activity in brown adipose tissue from pregnant and lactating rats. Biochem Biophys Res Commun. 1986 Aug 14;138(3):1315–1321. doi: 10.1016/s0006-291x(86)80426-0. [DOI] [PubMed] [Google Scholar]
  5. Glick Z., Wu S. Y., Lupien J., Reggio R., Bray G. A., Fisher D. A. Meal-induced brown fat thermogenesis and thyroid hormone metabolism in rats. Am J Physiol. 1985 Nov;249(5 Pt 1):E519–E524. doi: 10.1152/ajpendo.1985.249.5.E519. [DOI] [PubMed] [Google Scholar]
  6. Hefco E., Krulich L., Illner P., Larsen P. R. Effect of acute exposure to cold on the activity of the hypothalamic-pituitary-thyroid system. Endocrinology. 1975 Nov;97(5):1185–1195. doi: 10.1210/endo-97-5-1185. [DOI] [PubMed] [Google Scholar]
  7. Himms-Hagen J., Desautels M. A mitochondrial defect in brown adipose tissue of the obese (ob/ob) mouse: reduced binding of purine nucleotides and a failure to respond to cold by an increase in binding. Biochem Biophys Res Commun. 1978 Jul 28;83(2):628–634. doi: 10.1016/0006-291x(78)91036-7. [DOI] [PubMed] [Google Scholar]
  8. Jones R. G., Williamson D. H. Alterations in mammary-gland blood flow and glucose metabolism in the lactating rat induced by short-term starvation and refeeding. Biosci Rep. 1984 May;4(5):421–426. doi: 10.1007/BF01122507. [DOI] [PubMed] [Google Scholar]
  9. Kates A. L., Himms-Hagen J. Defective cold-induced stimulation of thyroxine 5'-deiodinase in brown adipose tissue of the genetically obese (ob/ob) mouse. Biochem Biophys Res Commun. 1985 Jul 16;130(1):188–193. doi: 10.1016/0006-291x(85)90400-0. [DOI] [PubMed] [Google Scholar]
  10. Kochupillai N., Yalow R. S. Preparation, purification, and stability of high specific activity 125I-labeled thyronines. Endocrinology. 1978 Jan;102(1):128–135. doi: 10.1210/endo-102-1-128. [DOI] [PubMed] [Google Scholar]
  11. Larsen P. R., Silva J. E., Kaplan M. M. Relationships between circulating and intracellular thyroid hormones: physiological and clinical implications. Endocr Rev. 1981 Winter;2(1):87–102. doi: 10.1210/edrv-2-1-87. [DOI] [PubMed] [Google Scholar]
  12. Leonard J. L., Mellen S. A., Larsen P. R. Thyroxine 5'-deiodinase activity in brown adipose tissue. Endocrinology. 1983 Mar;112(3):1153–1155. doi: 10.1210/endo-112-3-1153. [DOI] [PubMed] [Google Scholar]
  13. Leonard J. L., Rosenberg I. N. Iodothyronine 5'-deiodinase from rat kidney: substrate specificity and the 5'-deiodination of reverse triiodothyronine. Endocrinology. 1980 Nov;107(5):1376–1383. doi: 10.1210/endo-107-5-1376. [DOI] [PubMed] [Google Scholar]
  14. Obregon M. J., Pascual A., de Escobar G. M., Escobar del Rey F. Pituitary and plasma thyrotropin, thyroxine, and triiodothyronine after hyperthyroidism. Endocrinology. 1979 May;104(5):1467–1473. doi: 10.1210/endo-104-5-1467. [DOI] [PubMed] [Google Scholar]
  15. Rothwell N. J., Saville M. E., Stock M. J. Brown fat activity in fasted and refed rats. Biosci Rep. 1984 Apr;4(4):351–357. doi: 10.1007/BF01140499. [DOI] [PubMed] [Google Scholar]
  16. Silva J. E., Gordon M. B., Crantz F. R., Leonard J. L., Larsen P. R. Qualitative and quantitative differences in the pathways of extrathyroidal triiodothyronine generation between euthyroid and hypothyroid rats. J Clin Invest. 1984 Apr;73(4):898–907. doi: 10.1172/JCI111313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Silva J. E., Larsen P. R. Adrenergic activation of triiodothyronine production in brown adipose tissue. Nature. 1983 Oct 20;305(5936):712–713. doi: 10.1038/305712a0. [DOI] [PubMed] [Google Scholar]
  18. Silva J. E., Larsen P. R. Potential of brown adipose tissue type II thyroxine 5'-deiodinase as a local and systemic source of triiodothyronine in rats. J Clin Invest. 1985 Dec;76(6):2296–2305. doi: 10.1172/JCI112239. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Silva J. E., Leonard J. L., Crantz F. R., Larsen P. R. Evidence for two tissue-specific pathways for in vivo thyroxine 5'-deiodination in the rat. J Clin Invest. 1982 May;69(5):1176–1184. doi: 10.1172/JCI110554. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Silva J. E., Matthews P. Thyroid hormone metabolism and the source of plasma triiodothyronine in 2-week-old rats: effects of thyroid status. Endocrinology. 1984 Jun;114(6):2394–2405. doi: 10.1210/endo-114-6-2394. [DOI] [PubMed] [Google Scholar]
  21. Villarroya F., Felipe A., Mampel T. Sequential changes in brown adipose tissue composition, cytochrome oxidase activity and GDP binding throughout pregnancy and lactation in the rat. Biochim Biophys Acta. 1986 Jun 19;882(2):187–191. doi: 10.1016/0304-4165(86)90154-6. [DOI] [PubMed] [Google Scholar]
  22. Visser T. J., Kaplan M. M., Leonard J. L., Larsen P. R. Evidence for two pathways of iodothyronine 5'-deiodination in rat pituitary that differ in kinetics, propylthiouracil sensitivity, and response to hypothyroidism. J Clin Invest. 1983 Apr;71(4):992–1002. doi: 10.1172/JCI110854. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Visser T. J., Leonard J. L., Kaplan M. M., Larsen P. R. Kinetic evidence suggesting two mechanisms for iodothyronine 5'-deiodination in rat cerebral cortex. Proc Natl Acad Sci U S A. 1982 Aug;79(16):5080–5084. doi: 10.1073/pnas.79.16.5080. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Visser T. J. Mechanism of action of iodothyronine-5'-deiodinase. Biochim Biophys Acta. 1979 Aug 15;569(2):302–308. doi: 10.1016/0005-2744(79)90066-4. [DOI] [PubMed] [Google Scholar]
  25. Wang C., Smith R. L. Lowry determination of protein in the presence of Triton X-100. Anal Biochem. 1975 Feb;63(2):414–417. doi: 10.1016/0003-2697(75)90363-2. [DOI] [PubMed] [Google Scholar]
  26. van Hardeveld C., Zuidwijk M. J., Kassenaar A. A. Studies on the origin of altered thyroid hormone levels in the blood of rats during cold exposure. II. Effect of propranolol and chemical sympathectomy. Acta Endocrinol (Copenh) 1979 Jul;91(3):484–492. doi: 10.1530/acta.0.0910484. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES