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. 1987 Apr 15;243(2):597–601. doi: 10.1042/bj2430597

Cloning and sequencing of the medium-chain S-acyl fatty acid synthetase thioester hydrolase cDNA from rat mammary gland.

J Naggert, B Williams, D P Cashman, S Smith
PMCID: PMC1147896  PMID: 3632637

Abstract

cDNA clones coding for the medium-chain S-acyl fatty acid synthetase thioester hydrolase (thioesterase II) from rat mammary gland were identified in a bacteriophage lambda gt11 library and their nucleotide sequences were determined. The predicted coding region spans 263 amino acid residues and includes a sequence identical with that of a peptide derived from the enzyme active site. The rat thioesterase II cDNA sequence exhibits homology with that of a thioesterase found in duck uropygial glands.

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Selected References

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  1. Birnstiel M. L., Busslinger M., Strub K. Transcription termination and 3' processing: the end is in site! Cell. 1985 Jun;41(2):349–359. doi: 10.1016/s0092-8674(85)80007-6. [DOI] [PubMed] [Google Scholar]
  2. Feinberg A. P., Vogelstein B. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum. Anal Biochem. 1984 Feb;137(1):266–267. doi: 10.1016/0003-2697(84)90381-6. [DOI] [PubMed] [Google Scholar]
  3. Gross R. H. A DNA sequence analysis program for the Apple Macintosh. Nucleic Acids Res. 1986 Jan 10;14(1):591–596. doi: 10.1093/nar/14.1.591. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hattori M., Sakaki Y. Dideoxy sequencing method using denatured plasmid templates. Anal Biochem. 1986 Feb 1;152(2):232–238. doi: 10.1016/0003-2697(86)90403-3. [DOI] [PubMed] [Google Scholar]
  5. Heinzel R., Appelhans H., Gassen G., Seemüller U., Machleidt W., Fritz H., Steffens G. Molecular cloning and expression of cDNA for human antileukoprotease from cervix uterus. Eur J Biochem. 1986 Oct 1;160(1):61–67. doi: 10.1111/j.1432-1033.1986.tb09940.x. [DOI] [PubMed] [Google Scholar]
  6. Knudsen J., Clark S., Dils R. Purification and some properties of a medium-chain acyl-thioester hydrolase from lactating-rabbit mammary gland which terminates chain elongation in fatty acid synthesis. Biochem J. 1976 Dec 15;160(3):683–691. doi: 10.1042/bj1600683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kozak M. Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs. Nucleic Acids Res. 1984 Jan 25;12(2):857–872. doi: 10.1093/nar/12.2.857. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kozak M. Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell. 1986 Jan 31;44(2):283–292. doi: 10.1016/0092-8674(86)90762-2. [DOI] [PubMed] [Google Scholar]
  9. Libertini L. J., Smith S. Purification and properties of a thioesterase from lactating rat mammary gland which modifies the product specificity of fatty acid synthetase. J Biol Chem. 1978 Mar 10;253(5):1393–1401. [PubMed] [Google Scholar]
  10. Libertini L. J., Smith S. Synthesis of long chain acyl-enzyme thioesters by modified fatty acid synthetases and their hydrolysis by a mammary gland thioesterase. Arch Biochem Biophys. 1979 Jan;192(1):47–60. doi: 10.1016/0003-9861(79)90070-5. [DOI] [PubMed] [Google Scholar]
  11. Locker J. Analytical and preparative electrophoresis of RNA in agarose-urea. Anal Biochem. 1979 Oct 1;98(2):358–367. doi: 10.1016/0003-2697(79)90154-4. [DOI] [PubMed] [Google Scholar]
  12. Messing J., Crea R., Seeburg P. H. A system for shotgun DNA sequencing. Nucleic Acids Res. 1981 Jan 24;9(2):309–321. doi: 10.1093/nar/9.2.309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mikkelsen J., Witkowski A., Smith S. Interaction of rat mammary gland thioesterase II with fatty acid synthetase is dependent on the presence of acyl chains on the synthetase. J Biol Chem. 1987 Feb 5;262(4):1570–1574. [PubMed] [Google Scholar]
  14. Okayama H., Berg P. High-efficiency cloning of full-length cDNA. Mol Cell Biol. 1982 Feb;2(2):161–170. doi: 10.1128/mcb.2.2.161. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pawlak J., Smith S. Evaluation of thioesterase II as a serum marker for rat mammary cancer. Cancer Res. 1986 Sep;46(9):4712–4719. [PubMed] [Google Scholar]
  16. Poulose A. J., Rogers L., Cheesbrough T. M., Kolattukudy P. E. Cloning and sequencing of the cDNA for S-acyl fatty acid synthase thioesterase from the uropygial gland of mallard duck. J Biol Chem. 1985 Dec 15;260(29):15953–15958. [PubMed] [Google Scholar]
  17. Proudfoot N. The end of the message and beyond. Nature. 1984 Feb 2;307(5950):412–413. doi: 10.1038/307412a0. [DOI] [PubMed] [Google Scholar]
  18. Randhawa Z. I., Naggert J., Blacher R. W., Smith S. Amino acid sequence of the serine active-site region of the medium-chain S-acyl fatty acid synthetase thioester hydrolase from rat mammary gland. Eur J Biochem. 1987 Feb 2;162(3):577–581. doi: 10.1111/j.1432-1033.1987.tb10678.x. [DOI] [PubMed] [Google Scholar]
  19. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]
  20. Young R. A., Davis R. W. Efficient isolation of genes by using antibody probes. Proc Natl Acad Sci U S A. 1983 Mar;80(5):1194–1198. doi: 10.1073/pnas.80.5.1194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Young R. A., Davis R. W. Yeast RNA polymerase II genes: isolation with antibody probes. Science. 1983 Nov 18;222(4625):778–782. doi: 10.1126/science.6356359. [DOI] [PubMed] [Google Scholar]

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