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. 1987 Jun 1;244(2):427–432. doi: 10.1042/bj2440427

The active sites of the beta-lactamases of Streptomyces cacaoi and Streptomyces albus G.

F De Meester 1, B Joris 1, M V Lenzini 1, P Dehottay 1, T Erpicium 1, J Dusart 1, D Klein 1, J M Ghuysen 1, J M Frère 1, J Van Beeumen 1
PMCID: PMC1148008  PMID: 2822004

Abstract

The active-site serine of the extracellular beta-lactamases of Streptomyces cacaoi and Streptomyces albus G has been labelled with beta-iodopenicillanate. The determination of the sequence of the labelled peptides obtained after trypsin digestion of the denatured proteins indicate both enzymes to be class A beta-lactamases. Surprisingly the two Streptomyces enzymes do not appear to be especially homologous, and none of them exhibited a high degree of homology with the Streptomyces R61 DD-peptidase. Our data confirm that, as a family of homologous enzymes, class A is rather heterogeneous, with only a small number of conserved residues in all members of the class.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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