Abstract
We have studied the effect of concanavalin A (Con A) on the rat electro-olfactogram response to several odorants. Each odorant was applied over a range of concentrations. For hydrophobic odorants whose response was affected by Con A, the diminution in response was maximal at odorant concentrations of about 1 microM in the olfactory mucus. The (odour) concentration-dependence of the change is compatible with the idea that Con A inactivates one or more types of olfactory receptor that normally bind odorants with dissociation constants of the order of 100 nM. With hydrophilic odorants we had to apply concentrations very much higher than this to elicit any response from the system. At these high concentrations we could observe Con A-induced diminutions in response.
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Selected References
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- Getchell T. V., Margolis F. L., Getchell M. L. Perireceptor and receptor events in vertebrate olfaction. Prog Neurobiol. 1984;23(4):317–345. doi: 10.1016/0301-0082(84)90008-x. [DOI] [PubMed] [Google Scholar]
- Menco B. P. Qualitative and quantitative freeze-fracture studies on olfactory and nasal respiratory structures of frog, ox, rat, and dog. I. A general survey. Cell Tissue Res. 1980;207(2):183–209. doi: 10.1007/BF00237805. [DOI] [PubMed] [Google Scholar]
- OTTOSON D. Analysis of the electrical activity of the olfactory epithelium. Acta Physiol Scand Suppl. 1955;35(122):1–83. [PubMed] [Google Scholar]
- Polak E. H. Mutiple profile-multiple receptor site model for vertebrate olfaction. J Theor Biol. 1973 Aug 22;40(3):469–484. doi: 10.1016/0022-5193(73)90005-2. [DOI] [PubMed] [Google Scholar]
- Senf W., Menco B. P., Punter P. H., Duyvesteyn P. Determination of odour affinities based on the dose-response relationships of the frog's electro-olfactogram. Experientia. 1980 Feb 15;36(2):213–215. doi: 10.1007/BF01953738. [DOI] [PubMed] [Google Scholar]
- Shirley S. G., Polak E. H., Mather R. A., Dodd G. H. The effect of concanavalin A on the rat electro-olfactogram. Differential inhibition of odorant response. Biochem J. 1987 Jul 1;245(1):175–184. doi: 10.1042/bj2450175. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TUCKER D. Physical variables in the olfactory stimulation process. J Gen Physiol. 1963 Jan;46:453–489. doi: 10.1085/jgp.46.3.453. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ueda T., Kobatake Y. Hydrophobicity of biosurfaces as shown by chemoreceptive thresholds in Tetrahymena, Physarum and Nitella. J Membr Biol. 1977 Jun 15;34(4):351–368. doi: 10.1007/BF01870308. [DOI] [PubMed] [Google Scholar]