Abstract
The cell wall of an alkalophilic strain of Bacillus sp. C-125 is composed of A1 gamma-peptidoglycan, a teichuronic acid and an unknown acidic polymer composed of glutamic acid and glucuronic acid, of which the molar ratio is approx. 4-5:1. Poly(gamma-L-glutamate) was prepared from the acidic polymer by removal of almost all of the glucuronic residues with trifluoromethanesulphonic acid treatment and purified chromatographically. The Mr of the polyglutamate preparation was estimated to be 14,000 by gel chromatography, or 43,000 on the basis of the content of N-terminal acid residues. The acidic polymer found in the cell wall of the organism was concluded to be a polyglutamate substituted with (oligo)glucuronic acid residues or a complex composed of two kinds of polymers (polyglutamate and polyglucuronate).
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Selected References
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- Aono R., Horikoshi K., Goto S. Composition of the peptidoglycan of alkalophilic Bacillus spp. J Bacteriol. 1984 Feb;157(2):688–689. doi: 10.1128/jb.157.2.688-689.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Aono R., Uramoto M. Presence of fucosamine in teichuronic acid of the alkalophilic Bacillus strain C-125. Biochem J. 1986 Jan 1;233(1):291–294. doi: 10.1042/bj2330291. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HANES C. S., HIRD F. J. R., ISHERWOOD F. A. Enzymic transpeptidation reactions involving gamma-glutamyl peptides and alpha-amino-acyl peptides. Biochem J. 1952 Apr;51(1):25–35. doi: 10.1042/bj0510025. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hanby W. E., Rydon H. N. The capsular substance of Bacillus anthracis: With an appendix by P. Bruce White. Biochem J. 1946;40(2):297–309. [PMC free article] [PubMed] [Google Scholar]
- THORNE C. B., GOMEZ C. G., NOYES H. E., HOUSEWRIGHT R. D. Production of glutamyl polypeptide by Bacillus subtilis. J Bacteriol. 1954 Sep;68(3):307–315. doi: 10.1128/jb.68.3.307-315.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]