TABLE 1.

Description and testing data of the confirmed acute Lyme disease samples from the Lyme Disease Biobank cohort^a,b

Sample ID	Patient origin	EM > 5 cm at enrollment	Antibiotic therapy at enrollment (days)	B. burgdorferi culture	B. burgdorferi culture fluid PCR	B. burgdorferi qPCR	Whole-cell lysate ELISA	C6 peptide ELISA	VlsE/ PepC10	Western blot IgM	Western blot IgG	Two-tier testing Result	Initial discriminatory model prediction	Tester Set Prediction
LYM-997	NY	YES	YES (1)	NA	NA	NEG	RE	POS	NA	POS	IT	POS	POS	NA
LYM-1237	NY	YES	NO	NA	NA	NEG	RE	POS	NA	POS	IT	POS	POS	NA
LYM-1232	WI	YES	YES (1)	NA	NA	NEG	NA	POS	POS	NEG	NEG	NEG	POS	NA
LYM-1227	NY	YES	NO	NO	POS	NEG	NR	NEG	NA	IT	NEG	NEG	POS	NA
LYM-1214	WI	YES	NO	NA	NA	NEG	NA	POS	NA	NEG	POS	POS	POS	NA
LYM-1206	NY	YES	NO	NA	NA	NEG	BL	POS	NA	POS	IT	POS	POS	NA
LYM-1203	NY	YES	NO	NO	POS	NEG	RE	POS	NA	IT	IT	NEG	POS	NA
LYM-1200	NY	YES	YES (1)	NO	POS	NEG	RE	NEG	NA	POS	IT	POS	NEG	NA
LYM-1199	NY	YES	NO	NO	NEG	NEG	NR	POS	NA	POS	IT	POS	NEG	NA
LYM-1191	NY	YES	YES (1)	NA	NA	NEG	RE	POS	NA	POS	IT	POS	POS	NA
LYM-1160	MA	YES	NO	NA	NA	NEG	RE	POS	NA	IT	IT	NEG	POS	POS
LYM-1131	MA	YES	NO	POS	POS	NEG	NR	NEG	NA	IT	IT	NEG	NEG	NA
LYM-1114	NY	YES	NO	NA	NA	POS	NR	POS	NA	IT	IT	NEG	NEG	NEG
LYM-1110	NY	YES	NO	NA	NA	POS	NR	NEG	NA	NEG	IT	NEG	POS	NA
LYM-1107	NY	YES	NO	NA	NA	NEG	RE	POS	NA	IT	POS	POS	POS	NA
LYM-1099	WI	YES	NO	NA	NA	POS	NA	NEG	NEG	NEG	NEG	NEG	NEG	NEG
LYM-1097	NY	YES	YES (10)	NA	NA	NEG	RE	POS	NA	POS	IT	POS	POS	POS
LYM-1093	WI	YES	YES (1)	NA	NA	NEG	NA	POS	POS	POS	NEG	POS	POS	NA
LYM-1034	WI	YES	YES (1)	NA	NA	NEG	NA	POS	POS	POS	NEG	POS	POS	NA
LYM-1031	NY	YES	NO	NA	NA	NEG	BL	POS	NA	IT	IT	NEG	POS	NA
LYM-1016	MA	YES	NO	NEG	NEG	NEG	RE	POS	NA	POS	POS	POS	POS	NA
LYM-1015	MA	YES	YES (1)	NEG	NEG	NEG	NR	POS	NA	POS	IT	POS	POS	NA
LYM-1005	NY	YES	NO	NA	NA	NEG	NR	POS	NA	POS	IT	POS	POS	POS
LYM-1002	NY	YES	NO	NA	NA	NEG	NR	POS	NA	POS	IT	POS	POS	NA
LYM-1001	NY	YES	NO	NA	NA	NEG	RE	POS	NA	IT	IT	NEG	POS	NA
LYM-998	NY	NO	NO	NA	NA	NEG	RE	POS	NA	POS	IT	POS	POS	POS
LYM-1207	WI	NO	NO	NA	NA	NEG	NA	POS	NA	NEG	NEG	NEG	POS	NA
LYM-1181	NY	NO	NO	NA	NA	NEG	NR	POS	NA	POS	IT	POS	POS	NA
LYM-1127	NY	NO	YES (1)	NEG	NEG	NEG	RE	POS	NA	IT	POS	POS	POS	NA
LYM-1055	NY	NO	NO	NA	NA	NEG	BL	POS	NA	POS	IT	POS	POS	NA
LYM-1204	NY	NO	NO	NA	NA	NEG	NR	POS	NA	POS	IT	POS	NEG	NA
LYM-1137	MA	NO	NO	NEG	NEG	NEG	RE	POS	NA	POS	POS	POS	POS	NA
LYM-1123	NY	NO	NO	NEG	NEG	NA	NR	POS	NA	POS	NEG	POS	POS	NA
LYM-1086	WI	NO	YES (2)	NA	NA	NEG	NA	POS	POS	POS	NEG	POS	POS	NA
LYM-1080	WI	NO	NO	NA	NA	NEG	NA	POS	POS	POS	POS	POS	POS	NA
LYM-1022	WI	NO	YES (2)	NA	NA	NEG	NA	NEG	POS	POS	NEG	POS	POS	POS
LYM-1013	NY	NO	NO	NA	NA	POS	BL	NEG	NA	NEG	IT	NEG	NEG	NA
LYM-1010	NY	NO	NO	NA	NA	NEG	RE	NEG	NA	POS	IT	POS	POS	NA

^a NA: data not available; RE: reactive; NR: not reactive; IT: indeterminate; BL: borderline; POS: positive; NEG: negative; NY; New York; WI: Wisconsin; MA: Massachussetts.

^b Samples with an infection identified by qPCR and/or culture are shown in bold. B. burgdorferi qPCR was performed in whole blood samples.