Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1987 Aug 15;246(1):89–95. doi: 10.1042/bj2460089

Stimulation by D-glucose of mitochondrial oxidative events in islet cells.

A Sener 1, W J Malaisse 1
PMCID: PMC1148243  PMID: 3314867

Abstract

D-Glucose catabolism was examined in rat pancreatic islets and tumoral insulin-producing cells (RINm5F line) exposed to D-[5-3H]glucose, D-[3,4-14C]glucose or D-[6-14C]glucose. In normal islets, a rise in D-glucose concentration from 2.8 to 16.7 mM augmented (i) the transfer of NADH, presumably via the glycerol phosphate shuttle, into the mitochondria, (ii) the decarboxylation of pyruvate and (iii) the oxidation of acetyl residues, even when the flow rates were expressed relative to the total rate of D-glucose utilization. Islet cells are thus organized to favour those mitochondrial oxidative events yielding the major fraction of the energy derived from the catabolism of D-glucose. In tumoral islet cells of the RINm5F line, all three oxidative mitochondrial processes, when expressed relative to the overall rate of D-glucose utilization, occurred at a low rate and failed to increase in response to the rise in D-glucose concentration. These findings emphasize the importance of distal regulatory processes in the control of D-glucose catabolism in normal islet cells, and document the perturbation of such processes in a model of dysfunction for this fuel-sensor organ.

Full text

PDF
89

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ashcroft S. J., Weerasinghe L. C., Bassett J. M., Randle P. J. The pentose cycle and insulin release in mouse pancreatic islets. Biochem J. 1972 Feb;126(3):525–532. doi: 10.1042/bj1260525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Carpinelli A. R., Sener A., Herchuelz A., Malaisse W. J. Stimulus-secretion coupling of glucose-induced insulin release. Effect of intracellular acidification upon calcium efflux from islet cells. Metabolism. 1980 Jun;29(6):540–545. doi: 10.1016/0026-0495(80)90079-7. [DOI] [PubMed] [Google Scholar]
  3. Giroix M. H., Dufrane S. P., Malaisse-Lagae F., Sener A., Malaisse W. J. Fasting-induced impairment of glucose-1,6-bisphosphate synthesis in pancreatic islets. Biochem Biophys Res Commun. 1984 Mar 15;119(2):543–548. doi: 10.1016/s0006-291x(84)80282-x. [DOI] [PubMed] [Google Scholar]
  4. Giroix M. H., Malaisse-Lagae F., Sener A., Malaisse W. J. Hexose metabolism in pancreatic islets. Galactose transport, phosphorylation and oxidation. Mol Cell Biochem. 1985 Feb;66(1):61–64. doi: 10.1007/BF00231824. [DOI] [PubMed] [Google Scholar]
  5. Giroix M. H., Sener A., Dufrane S. P., Malaisse-Lagae F., Malaisse W. J. Glucose metabolism in insulin-producing tumoral cells. Arch Biochem Biophys. 1985 Sep;241(2):561–570. doi: 10.1016/0003-9861(85)90581-8. [DOI] [PubMed] [Google Scholar]
  6. Giroix M. H., Sener A., Malaisse W. J. Pentose cycle pathway in normal and tumoral islet cells. FEBS Lett. 1985 Jun 3;185(1):1–3. doi: 10.1016/0014-5793(85)80728-6. [DOI] [PubMed] [Google Scholar]
  7. Gylfe E., Andersson T., Rorsman P., Abrahamsson H., Arkhammar P., Hellman P., Hellman B., Oie H. K., Gazdar A. F. Depolarization-independent net uptake of calcium into clonal insulin-releasing cells exposed to glucose. Biosci Rep. 1983 Oct;3(10):927–937. doi: 10.1007/BF01140662. [DOI] [PubMed] [Google Scholar]
  8. Gylfe E., Hellman B. Role of glucose as a regulator and precursor of amino acids in the pancreatic beta-cells. Endocrinology. 1974 Apr;94(4):1150–1156. doi: 10.1210/endo-94-4-1150. [DOI] [PubMed] [Google Scholar]
  9. Halban P. A., Praz G. A., Wollheim C. B. Abnormal glucose metabolism accompanies failure of glucose to stimulate insulin release from a rat pancreatic cell line (RINm5F). Biochem J. 1983 May 15;212(2):439–443. doi: 10.1042/bj2120439. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hedeskov C. J. Mechanism of glucose-induced insulin secretion. Physiol Rev. 1980 Apr;60(2):442–509. doi: 10.1152/physrev.1980.60.2.442. [DOI] [PubMed] [Google Scholar]
  11. Hutton J. C., Malaisse W. J. Dynamics of O2 consumption in rat pancreatic islets. Diabetologia. 1980 May;18(5):395–405. doi: 10.1007/BF00276821. [DOI] [PubMed] [Google Scholar]
  12. KATZ J., WOOD H. G. The use of C14O2 yields from glucose-1- and -6-C14 for the evaluation of the pathways of glucose metabolism. J Biol Chem. 1963 Feb;238:517–523. [PubMed] [Google Scholar]
  13. Lebrun P., Malaisse W. J., Herchuelz A. Impairment by aminooxyacetate of ionic response to nutrients in pancreatic islets. Am J Physiol. 1983 Jul;245(1):E38–E46. doi: 10.1152/ajpendo.1983.245.1.E38. [DOI] [PubMed] [Google Scholar]
  14. Levy J., Herchuelz A., Sener A., Malaisse-Lagae F., Malaisse W. J. Cytochalasin B-induced impariment of glucose metabolism in islets of Langerhans. Endocrinology. 1976 Feb;98(2):429–437. doi: 10.1210/endo-98-2-429. [DOI] [PubMed] [Google Scholar]
  15. MacDonald M. J. Evidence for the malate aspartate shuttle in pancreatic islets. Arch Biochem Biophys. 1982 Feb;213(2):643–649. doi: 10.1016/0003-9861(82)90594-x. [DOI] [PubMed] [Google Scholar]
  16. MacDonald M. J. High content of mitochondrial glycerol-3-phosphate dehydrogenase in pancreatic islets and its inhibition by diazoxide. J Biol Chem. 1981 Aug 25;256(16):8287–8290. [PubMed] [Google Scholar]
  17. Malaisse W. J., Best L., Kawazu S., Malaisse-Lagae F., Sener A. The stimulus-secretion coupling of glucose-induced insulin release: fuel metabolism in islets deprived of exogenous nutrient. Arch Biochem Biophys. 1983 Jul 1;224(1):102–110. doi: 10.1016/0003-9861(83)90193-5. [DOI] [PubMed] [Google Scholar]
  18. Malaisse W. J., Carpinelli A. R., Sener A. Stimulus-secretion coupling of glucose-induced insulin release. Timing of early metabolic, ionic, and secretory events. Metabolism. 1981 May;30(5):527–532. doi: 10.1016/0026-0495(81)90191-8. [DOI] [PubMed] [Google Scholar]
  19. Malaisse W. J., Hutton J. C., Kawazu S., Sener A. The stimulus-secretion coupling of glucose-induced insulin release. Metabolic effects of menadione in isolated islets. Eur J Biochem. 1978 Jun 1;87(1):121–130. doi: 10.1111/j.1432-1033.1978.tb12357.x. [DOI] [PubMed] [Google Scholar]
  20. Malaisse W. J. Insulin release : the fuel concept. Diabete Metab. 1983 Dec;9(4):313–320. [PubMed] [Google Scholar]
  21. Malaisse W. J., Malaisse-Lagae F., Sener A. The stimulus-secretion coupling of glucose-induced insulin release: effect of aminooxyacetate upon nutrient-stimulated insulin secretion. Endocrinology. 1982 Aug;111(2):392–397. doi: 10.1210/endo-111-2-392. [DOI] [PubMed] [Google Scholar]
  22. Malaisse W. J., Sener A., Carpinelli A. R., Anjaneyulu K., Lebrun P., Herchuelz A., Christophe J. The stimulus-secretion coupling of glucose-induced insulin release. XLVI. Physiological role of L-glutamine as a fuel for pancreatic islets. Mol Cell Endocrinol. 1980 Nov;20(2):171–189. doi: 10.1016/0303-7207(80)90080-5. [DOI] [PubMed] [Google Scholar]
  23. Malaisse W. J., Sener A., Herchuelz A., Hutton J. C. Insulin release: the fuel hypothesis. Metabolism. 1979 Apr;28(4):373–386. doi: 10.1016/0026-0495(79)90111-2. [DOI] [PubMed] [Google Scholar]
  24. Meglasson M. D., Matschinsky F. M. New perspectives on pancreatic islet glucokinase. Am J Physiol. 1984 Jan;246(1 Pt 1):E1–13. doi: 10.1152/ajpendo.1984.246.1.E1. [DOI] [PubMed] [Google Scholar]
  25. Sener A., Kawazu S., Hutton J. C., Boschero A. C., Devis G., Somers G., Herchuelz A., Malaisse W. J. The stimulus-secretion coupling of glucose-induced insulin release. Effect of exogenous pyruvate on islet function. Biochem J. 1978 Oct 15;176(1):217–232. doi: 10.1042/bj1760217. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Sener A., Leclercq-Meyer V., Giroix M. H., Malaisse W. J., Hellerström C. Opposite effects of D-glucose and a nonmetabolized analogue of L-leucine on respiration and secretion in insulin-producing tumoral cells (RINm5F). Diabetes. 1987 Feb;36(2):187–192. doi: 10.2337/diab.36.2.187. [DOI] [PubMed] [Google Scholar]
  27. Sener A., Malaisse-Lagae F., Dufrane S. P., Malaisse W. J. The coupling of metabolic to secretory events in pancreatic islets. The cytosolic redox state. Biochem J. 1984 Jun 1;220(2):433–440. doi: 10.1042/bj2200433. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Sener A., Malaisse W. J. The metabolism of glucose in pancreatic islets. Diabete Metab. 1978 Jun;4(2):127–133. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES