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. 1987 Sep 15;246(3):619–623. doi: 10.1042/bj2460619

Phorbol-ester-induced alterations of free calcium ion transients in single rat hepatocytes.

N M Woods 1, K S Cuthbertson 1, P H Cobbold 1
PMCID: PMC1148325  PMID: 3479980

Abstract

The effect of the phorbol esters phorbol 12-myristate 13-acetate (TPA) and phorbol 12,13-dibutyrate (PDB) on changes in free Ca2+ concentration ([Ca2+]i) in single rat hepatocytes, microinjected with the photoprotein aequorin, were investigated. [Arg8]vasopressin and phenylephrine induced a series of repetitive [Ca2+]i transients. Phorbol esters inhibited the alpha 1-adrenoceptor-induced response; sub-nanomolar concentrations decreased the transient frequency, and higher concentrations abolished the transients. The inhibitory effect of PDB was readily reversible. Phorbol esters were less effective in decreasing the frequency of [Arg8]-vasopressin-induced transients, and the inhibition could be overcome by high [Arg8]vasopressin concentrations.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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