Abstract
A glutathione transferase from human mononuclear leucocytes with high activity towards trans-stilbene oxide (GT-tSBO) was purified. GT-tSBO is expressed in only about 50% of the individuals studied. As judged from activity measurements, immunological studies and the fact that only those individuals who express glutathione transferase mu have high activity towards trans-stilbene oxide, it is concluded that the hepatic transferase mu is identical with the glutathione transferase (GT-tSBO) in mononuclear leucocytes.
Full text
PDF


Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Board P. G. Biochemical genetics of glutathione-S-transferase in man. Am J Hum Genet. 1981 Jan;33(1):36–43. [PMC free article] [PubMed] [Google Scholar]
- Burnette W. N. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem. 1981 Apr;112(2):195–203. doi: 10.1016/0003-2697(81)90281-5. [DOI] [PubMed] [Google Scholar]
- Gill S. S., Ota K., Hammock B. D. Radiometric assays for mammalian epoxide hydrolases and glutathione S-transferase. Anal Biochem. 1983 May;131(1):273–282. doi: 10.1016/0003-2697(83)90166-5. [DOI] [PubMed] [Google Scholar]
- Habig W. H., Pabst M. J., Jakoby W. B. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem. 1974 Nov 25;249(22):7130–7139. [PubMed] [Google Scholar]
- Mannervik B., Alin P., Guthenberg C., Jensson H., Tahir M. K., Warholm M., Jörnvall H. Identification of three classes of cytosolic glutathione transferase common to several mammalian species: correlation between structural data and enzymatic properties. Proc Natl Acad Sci U S A. 1985 Nov;82(21):7202–7206. doi: 10.1073/pnas.82.21.7202. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mannervik B., Guthenberg C. Glutathione transferase (human placenta). Methods Enzymol. 1981;77:231–235. doi: 10.1016/s0076-6879(81)77030-7. [DOI] [PubMed] [Google Scholar]
- Mannervik B. The isoenzymes of glutathione transferase. Adv Enzymol Relat Areas Mol Biol. 1985;57:357–417. doi: 10.1002/9780470123034.ch5. [DOI] [PubMed] [Google Scholar]
- Seidegård J., DePierre J. W., Pero R. W. Hereditary interindividual differences in the glutathione transferase activity towards trans-stilbene oxide in resting human mononuclear leukocytes are due to a particular isozyme(s). Carcinogenesis. 1985 Aug;6(8):1211–1216. doi: 10.1093/carcin/6.8.1211. [DOI] [PubMed] [Google Scholar]
- Seidegård J., Pero R. W., Miller D. G., Beattie E. J. A glutathione transferase in human leukocytes as a marker for the susceptibility to lung cancer. Carcinogenesis. 1986 May;7(5):751–753. doi: 10.1093/carcin/7.5.751. [DOI] [PubMed] [Google Scholar]
- Seidegård J., Pero R. W. The hereditary transmission of high glutathione transferase activity towards trans-stilbene oxide in human mononuclear leukocytes. Hum Genet. 1985;69(1):66–68. doi: 10.1007/BF00295531. [DOI] [PubMed] [Google Scholar]
- Strange R. C., Faulder C. G., Davis B. A., Hume R., Brown J. A., Cotton W., Hopkinson D. A. The human glutathione S-transferases: studies on the tissue distribution and genetic variation of the GST1, GST2 and GST3 isozymes. Ann Hum Genet. 1984 Jan;48(Pt 1):11–20. doi: 10.1111/j.1469-1809.1984.tb00829.x. [DOI] [PubMed] [Google Scholar]
- Warholm M., Guthenberg C., Mannervik B. Molecular and catalytic properties of glutathione transferase mu from human liver: an enzyme efficiently conjugating epoxides. Biochemistry. 1983 Jul 19;22(15):3610–3617. doi: 10.1021/bi00284a011. [DOI] [PubMed] [Google Scholar]
- Warholm M., Guthenberg C., Mannervik B., von Bahr C., Glaumann H. Identification of a new glutathione S-transferase in human liver. Acta Chem Scand B. 1980;34(8):607–621. doi: 10.3891/acta.chem.scand.34b-0607. [DOI] [PubMed] [Google Scholar]
- Warholm M., Guthenberg C., Mannervik B., von Bahr C. Purification of a new glutathione S-transferase (transferase mu) from human liver having high activity with benzo(alpha)pyrene-4,5-oxide. Biochem Biophys Res Commun. 1981 Jan 30;98(2):512–519. doi: 10.1016/0006-291x(81)90870-6. [DOI] [PubMed] [Google Scholar]
- Warholm M., Guthenberg C., von Bahr C., Mannervik B. Glutathione transferases from human liver. Methods Enzymol. 1985;113:499–504. doi: 10.1016/s0076-6879(85)13065-x. [DOI] [PubMed] [Google Scholar]
- von Bahr C., Groth C. G., Jansson H., Lundgren G., Lind M., Glaumann H. Drug metabolism in human liver in vitro: establishment of a human liver bank. Clin Pharmacol Ther. 1980 Jun;27(6):711–725. doi: 10.1038/clpt.1980.102. [DOI] [PubMed] [Google Scholar]