Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1987 Oct 1;247(1):237–239. doi: 10.1042/bj2470237

A simple procedure for the isolation of protein disulphide-isomerase.

J Koivu 1, R Myllylä 1, K I Kivirikko 1
PMCID: PMC1148394  PMID: 3689349

Abstract

A two-step procedure is described for the purification of protein disulphide-isomerase (PDI). This procedure is based on the previous finding that the beta-subunit of the prolyl 4-hydroxylase tetramer (alpha 2 beta 2) is identical with PDI [Koivu, Myllylä, Helaakoski, Pihlajaniemi, Tasanen & Kivirikko (1987) J. Biol. Chem. 262, 6447-6449; Pihlajaniemi, Helaakoski, Tasanen, Myllylä, Huhtala, Koivu & Kivirikko (1987) EMBO J. 6, 643-649]. The procedure involves purification of the prolyl 4-hydroxylase tetramer by a simple affinity chromatography and subsequent isolation of the beta-subunit from the dissociated tetramer by ion-exchange chromatography.

Full text

PDF
237

Images in this article

239Fig. 2.
on p.239

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anfinsen C. B., Scheraga H. A. Experimental and theoretical aspects of protein folding. Adv Protein Chem. 1975;29:205–300. doi: 10.1016/s0065-3233(08)60413-1. [DOI] [PubMed] [Google Scholar]
  2. Berg R. A., Kedersha N. L., Guzman N. A. Purification and partial characterization of the two nonidentical subunits of prolyl hydroxylase. J Biol Chem. 1979 Apr 25;254(8):3111–3118. [PubMed] [Google Scholar]
  3. Bjelland S., Wallevik K., Krøll J., Dixon J. E., Morin J. E., Freedman R. B., Lambert N., Varandani P. T., Nafz M. A. Immunological identity between bovine preparations of thiol:protein-disulphide oxidoreductase, glutathione-insulin transhydrogenase and protein-disulphide isomerase. Biochim Biophys Acta. 1983 Sep 28;747(3):197–199. doi: 10.1016/0167-4838(83)90097-3. [DOI] [PubMed] [Google Scholar]
  4. Carmichael D. F., Morin J. E., Dixon J. E. Purification and characterization of a thiol:protein disulfide oxidoreductase from bovine liver. J Biol Chem. 1977 Oct 25;252(20):7163–7167. [PubMed] [Google Scholar]
  5. Edman J. C., Ellis L., Blacher R. W., Roth R. A., Rutter W. J. Sequence of protein disulphide isomerase and implications of its relationship to thioredoxin. Nature. 1985 Sep 19;317(6034):267–270. doi: 10.1038/317267a0. [DOI] [PubMed] [Google Scholar]
  6. Freedman R. B., Brockway B. E., Lambert N. Protein disulphide-isomerase and the formation of native disulphide bonds. Biochem Soc Trans. 1984 Dec;12(6):929–932. doi: 10.1042/bst0120929. [DOI] [PubMed] [Google Scholar]
  7. Hillson D. A., Lambert N., Freedman R. B. Formation and isomerization of disulfide bonds in proteins: protein disulfide-isomerase. Methods Enzymol. 1984;107:281–294. doi: 10.1016/0076-6879(84)07018-x. [DOI] [PubMed] [Google Scholar]
  8. Ibbetson A. L., Freedman R. B. Thiol-protein disulphide oxidoreductases. Assay of microsomal membrane-bound glutathione-insulin transhydrogenase and comparison with protein disulphide-isomerase. Biochem J. 1976 Nov;159(2):377–384. doi: 10.1042/bj1590377. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kaetzel C. S., Rao C. K., Lamm M. E. Protein disulphide-isomerase from human placenta and rat liver. Purification and immunological characterization with monoclonal antibodies. Biochem J. 1987 Jan 1;241(1):39–47. doi: 10.1042/bj2410039. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kao W. W., Berg R. A., Prockop D. J. Ascorbate increases the synthesis of procollagen hydroxyproline by cultured fibroblasts from chick embryo tendons without activation of prolyl hydroxyla. Biochim Biophys Acta. 1975 Dec 5;411(2):202–215. doi: 10.1016/0304-4165(75)90300-1. [DOI] [PubMed] [Google Scholar]
  11. Kedersha N. L., Berg R. A. An improved method for the purification of vertebrate prolyl hydroxylase by affinity chromatography. Coll Relat Res. 1981 Jul;1(4):345–353. doi: 10.1016/s0174-173x(81)80011-8. [DOI] [PubMed] [Google Scholar]
  12. Kedersha N. L., Broek D., Berg R. A. A novel isoform of cytoplasmic actin that binds poly-L-proline. Biochem J. 1986 Sep 1;238(2):561–570. doi: 10.1042/bj2380561. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kivirikko K. I., Myllylä R. Posttranslational enzymes in the biosynthesis of collagen: intracellular enzymes. Methods Enzymol. 1982;82(Pt A):245–304. doi: 10.1016/0076-6879(82)82067-3. [DOI] [PubMed] [Google Scholar]
  14. Koivu J., Myllylä R., Helaakoski T., Pihlajaniemi T., Tasanen K., Kivirikko K. I. A single polypeptide acts both as the beta subunit of prolyl 4-hydroxylase and as a protein disulfide-isomerase. J Biol Chem. 1987 May 15;262(14):6447–6449. [PubMed] [Google Scholar]
  15. Koivu J., Myllylä R. Protein disulfide-isomerase retains procollagen prolyl 4-hydroxylase structure in its native conformation. Biochemistry. 1986 Oct 7;25(20):5982–5986. doi: 10.1021/bi00368a022. [DOI] [PubMed] [Google Scholar]
  16. Lambert N., Freedman R. B. Kinetics and specificity of homogeneous protein disulphide-isomerase in protein disulphide isomerization and in thiol-protein-disulphide oxidoreduction. Biochem J. 1983 Jul 1;213(1):235–243. doi: 10.1042/bj2130235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. McGee J. O., Langness U., Udenfriend S. Immunological evidence for an inactive precursor of collagen proline hydroxylase in cultured fibroblasts. Proc Natl Acad Sci U S A. 1971 Jul;68(7):1585–1589. doi: 10.1073/pnas.68.7.1585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Myllylä R., Oikarinen J. A rapid and sensitive assay for protein disulphide isomerase activity. J Biochem Biophys Methods. 1983 Feb;7(2):115–121. doi: 10.1016/0165-022x(83)90045-3. [DOI] [PubMed] [Google Scholar]
  19. Pihlajaniemi T., Helaakoski T., Tasanen K., Myllylä R., Huhtala M. L., Koivu J., Kivirikko K. I. Molecular cloning of the beta-subunit of human prolyl 4-hydroxylase. This subunit and protein disulphide isomerase are products of the same gene. EMBO J. 1987 Mar;6(3):643–649. doi: 10.1002/j.1460-2075.1987.tb04803.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Stassen F. L., Cardinale G. J., McGee J. O., Udenfriend S. Prolyl hydroxylase and an immunologically related protein in mammalian tissues. Arch Biochem Biophys. 1974 Jan;160(1):340–345. doi: 10.1016/s0003-9861(74)80042-1. [DOI] [PubMed] [Google Scholar]
  21. Tuderman L., Kuutti E. R., Kivirikko K. I. An affinity-column procedure using poly(L-proline) for the purification of prolyl hydroxylase. Purification of the enzyme from chick embryos. Eur J Biochem. 1975 Mar 3;52(1):9–16. doi: 10.1111/j.1432-1033.1975.tb03967.x. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES