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. 1988 Feb 15;250(1):197–201. doi: 10.1042/bj2500197

The influence of porphyrins on iron-catalysed generation of hydroxyl radicals.

J Van Steveninck 1, J P Boegheim 1, T M Dubbelman 1, J Van der Zee 1
PMCID: PMC1148832  PMID: 2833235

Abstract

Uroporphyrin I, haematoporphyrin and haematoporphyrin derivative had no effect on O2-. generation during oxidation of hypoxanthine by xanthine oxidase and on the formation of hydroxyl radicals (OH.) in the hypoxanthine/xanthine oxidase/Fe3+-EDTA/deoxyribose system. On the other hand, these porphyrins strongly inhibited O2-. formation in a horseradish peroxidase/H2O2/NADPH mixture, whereas they augmented OH. generation in this system after addition of Fe3+-EDTA. Experimental evidence suggests that these observations should be ascribed to the formation of a porphyrin anion radical in the horseradish peroxidase/NADPH system. The formation of this anion radical was confirmed by e.s.r. spectroscopy. This radical is apparently unable to reduce cytochrome c, but it can replace O2-. in the OH.-generating Haber-Weiss reaction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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