Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1988 Mar 1;250(2):443–446. doi: 10.1042/bj2500443

The fatty-acid-induced conformational states of human serum albumin investigated by means of multiple co-binding of protons and oleic acid.

J H Dröge 1, L H Janssen 1, J Wilting 1
PMCID: PMC1148876  PMID: 2833243

Abstract

The binding of oleic acid to human serum albumin causes progressive changes in (a) the pK of some amino acid residues, as detected by pH-stat titration and (b) the induced molar ellipticities of albumin-bound drugs (diazepam and oxyphenbutazone), as measured by c.d. It is concluded that albumin undergoes several conformational transitions as the amount of oleic acid bound increases from 0 to about 9 molecules/molecule of protein. At least three different conformations of the protein seem to be involved. These conformations can be linked with the three classes of oleic acid-binding sites on albumin.

Full text

PDF
443

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ashbrook J. D., Spector A. A., Santos E. C., Fletcher J. E. Long chain fatty acid binding to human plasma albumin. J Biol Chem. 1975 Mar 25;250(6):2333–2338. [PubMed] [Google Scholar]
  2. Brodersen R., Sjödin T., Sjöholm I. Independent binding of ligands to human serum albumin. J Biol Chem. 1977 Jul 25;252(14):5067–5072. [PubMed] [Google Scholar]
  3. Dröge J. H., Janssen L. H., Wilting J. A comparative study of some physico-chemical properties of human serum albumin samples from different sources--I. Some physico-chemical properties of isoionic human serum albumin solutions. Biochem Pharmacol. 1982 Dec 1;31(23):3775–3779. doi: 10.1016/0006-2952(82)90292-1. [DOI] [PubMed] [Google Scholar]
  4. Dröge J. H., Janssen L. H., Wilting J. Allosteric properties of the oxyphenbutazone--human serum albumin complex. Pharm Weekbl Sci. 1983 Oct 21;5(5):228–233. doi: 10.1007/BF02332949. [DOI] [PubMed] [Google Scholar]
  5. Dröge J. H., Janssen L. H., Wilting J. Evidence for the fatty acid-induced heterogeneity of the N and B conformations of human serum albumin. Biochem Pharmacol. 1985 Sep 15;34(18):3299–3304. doi: 10.1016/0006-2952(85)90349-1. [DOI] [PubMed] [Google Scholar]
  6. Fehske K. J., Müller W. E., Wollert U. A highly reactive tyrosine residue as part of the indole and benzodiazepine binding site of human serum albumin. Biochim Biophys Acta. 1979 Apr 25;577(2):346–359. doi: 10.1016/0005-2795(79)90038-2. [DOI] [PubMed] [Google Scholar]
  7. Fehske K. J., Müller W. E., Wollert U. The location of drug binding sites in human serum albumin. Biochem Pharmacol. 1981 Apr 1;30(7):687–692. doi: 10.1016/0006-2952(81)90151-9. [DOI] [PubMed] [Google Scholar]
  8. Harmsen B. J., De Bruin S. H., Janssen L. H., Rodrigues de Miranda J. F., Van Os G. A. pK change of imidazole groups in bovine serum albumin due to the conformational change at neutral pH. Biochemistry. 1971 Aug 17;10(17):3217–3221. doi: 10.1021/bi00793a009. [DOI] [PubMed] [Google Scholar]
  9. Janssen L. H., Van Wilgenburg M. T. Experimental observation of ionic forces in drug-protein interactions as illustrated by the binding of sulfaethidole by human serum albumin. Mol Pharmacol. 1978 Sep;14(5):884–889. [PubMed] [Google Scholar]
  10. Janssen L. H., Van Wilgenburg M. T., Wilting J. Human serum albumin as an allosteric two-state protein. Evidence from effects of calcium and warfarin on proton binding behaviour. Biochim Biophys Acta. 1981 Jul 28;669(2):244–250. doi: 10.1016/0005-2795(81)90247-6. [DOI] [PubMed] [Google Scholar]
  11. Janssen L. H., de Bruin S. H., van Os G. A. H+ titration studies on human hemoglobin. Biochim Biophys Acta. 1970 Nov 17;221(2):214–227. doi: 10.1016/0005-2795(70)90261-8. [DOI] [PubMed] [Google Scholar]
  12. Kragh-Hansen U. Molecular aspects of ligand binding to serum albumin. Pharmacol Rev. 1981 Mar;33(1):17–53. [PubMed] [Google Scholar]
  13. LEONARD W. J., Jr, VIJAI K. K., FOSTER J. F. A structural transformation in bovine and human plasma albumins in alkaline solution as revealed by rotatory dispersion studies. J Biol Chem. 1963 Jun;238:1984–1988. [PubMed] [Google Scholar]
  14. Müller W. E., Wollert U. Human serum albumin as a 'silent receptor' for drugs and endogenous substances. Pharmacology. 1979;19(2):59–67. doi: 10.1159/000137289. [DOI] [PubMed] [Google Scholar]
  15. Simpson R. B., Ashbrook J. D., Santos E. C., Spector A. A. Partition of fatty acids. J Lipid Res. 1974 Jul;15(4):415–422. [PubMed] [Google Scholar]
  16. Sjöholm I., Ekman B., Kober A., Ljungstedt-Påhlman I., Seiving B., Sjödin T. Binding of drugs to human serum albumin:XI. The specificity of three binding sites as studied with albumin immobilized in microparticles. Mol Pharmacol. 1979 Nov;16(3):767–777. [PubMed] [Google Scholar]
  17. Spector A. A. Fatty acid binding to plasma albumin. J Lipid Res. 1975 May;16(3):165–179. [PubMed] [Google Scholar]
  18. Sudlow G., Birkett D. J., Wade D. N. Further characterization of specific drug binding sites on human serum albumin. Mol Pharmacol. 1976 Nov;12(6):1052–1061. [PubMed] [Google Scholar]
  19. Vallner J. J. Binding of drugs by albumin and plasma protein. J Pharm Sci. 1977 Apr;66(4):447–465. doi: 10.1002/jps.2600660402. [DOI] [PubMed] [Google Scholar]
  20. WYMAN J., Jr LINKED FUNCTIONS AND RECIPROCAL EFFECTS IN HEMOGLOBIN: A SECOND LOOK. Adv Protein Chem. 1964;19:223–286. doi: 10.1016/s0065-3233(08)60190-4. [DOI] [PubMed] [Google Scholar]
  21. Wanwimolruk S., Birkett D. J. The effects of N-B transition of human serum albumin on the specific drug-binding sites. Biochim Biophys Acta. 1982 Dec 20;709(2):247–255. doi: 10.1016/0167-4838(82)90467-8. [DOI] [PubMed] [Google Scholar]
  22. Wilting J., Hart B. J., De Gier J. J. The role of albumin conformation in the binding of diazepam to human serum albumin. Biochim Biophys Acta. 1980 Dec 16;626(2):291–298. doi: 10.1016/0005-2795(80)90123-3. [DOI] [PubMed] [Google Scholar]
  23. Wilting J., van der Giesen W. F., Janssen L. H. The effect of chloride on the binding of warfarin to albumin as a function of pH. Biochem Pharmacol. 1981 May 15;30(10):1025–1031. doi: 10.1016/0006-2952(81)90437-8. [DOI] [PubMed] [Google Scholar]
  24. Wilting J., van der Giesen W. F., Janssen L. H., Weideman M. M., Otagiri M., Perrin J. H. The effect of albumin conformation on the binding of warfarin to human serum albumin. The dependence of the binding of warfarin to human serum albumin on the hydrogen, calcium, and chloride ion concentrations as studied by circular dichroism, fluorescence, and equilibrium dialysis. J Biol Chem. 1980 Apr 10;255(7):3032–3037. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES