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. 1988 Mar 15;250(3):853–858. doi: 10.1042/bj2500853

1H-n.m.r. and c.d. studies of haem orientational disorder in sperm-whale myoglobin and human haemoglobin.

H S Aojula 1, M T Wilson 1, G R Moore 1, D J Williamson 1
PMCID: PMC1148933  PMID: 3390144

Abstract

1H-n.m.r. and c.d. studies on sperm-whale myoglobin show that the c.d. signal in the Soret region is inversely and linearly related to the proportion of minor isomer present. An alternative method, 'pH jump', is described for inducing orientational disorder in sperm-whale myoglobin without recourse to reconstitution. 1H-n.m.r. studies on human haemoglobin A indicate little heterogeneity in freshly isolated haemoglobin A, but the effect is enhanced in freeze-dried Sigma haemoglobin A.

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Selected References

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