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. 1988 Apr 1;251(1):301–304. doi: 10.1042/bj2510301

Characterization of atrial-natriuretic-factor-receptor-coupled membrane guanylate cyclase from rat and mouse testes.

R B Marala 1, R K Sharma 1
PMCID: PMC1148997  PMID: 2898940

Abstract

Studies with isolated adrenal cells and mouse testicular cells have supported a mediatory role of cyclic GMP in ANF (atrial natriuretic factor)-dependent steroidogenic signal transduction. This concept has been strengthened by the purification and biochemical characterization of a 180 kDa protein, which appears to contain both ANF receptor and guanylate cyclase activities, from rat adrenocortical carcinoma cells. Utilizing the antibody to 180 kDa membrane guanylate cyclase as a probe, we now demonstrate the direct presence of ANF-dependent membrane guanylate cyclase in mouse and rat testes. The antibody blocks the ANF-dependent guanylate cyclase activity in isolated membranes, and Western-blot analysis of the partially purified enzyme reveals a single 180 kDa protein. The presence of this enzyme in mouse and rat testes, together with its previous demonstration in rat adrenocortical carcinoma, represent an important potential biochemical role for this enzyme in receptor-mediated steroidogenic signal transduction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ahrens H., Paul A. K., Kuroda Y., Sharma R. K. Adrenocortical cyclic GMP-dependent protein kinase: purification, characterization, and modification of its activity by calmodulin, and its relationship with steroidogenesis. Arch Biochem Biophys. 1982 May;215(2):597–609. doi: 10.1016/0003-9861(82)90121-7. [DOI] [PubMed] [Google Scholar]
  2. Atarashi K., Mulrow P. J., Franco-Saenz R., Snajdar R., Rapp J. Inhibition of aldosterone production by an atrial extract. Science. 1984 Jun 1;224(4652):992–994. doi: 10.1126/science.6326267. [DOI] [PubMed] [Google Scholar]
  3. Atlas S. A., Laragh J. H. Atrial natriuretic peptide: a new factor in hormonal control of blood pressure and electrolyte homeostasis. Annu Rev Med. 1986;37:397–414. doi: 10.1146/annurev.me.37.020186.002145. [DOI] [PubMed] [Google Scholar]
  4. Bex F., Corbin A. Atrial natriuretic factor stimulates testosterone production by mouse interstitial cells. Eur J Pharmacol. 1985 Sep 10;115(1):125–126. doi: 10.1016/0014-2999(85)90595-3. [DOI] [PubMed] [Google Scholar]
  5. Cantin M., Genest J. The heart and the atrial natriuretic factor. Endocr Rev. 1985 Spring;6(2):107–127. doi: 10.1210/edrv-6-2-107. [DOI] [PubMed] [Google Scholar]
  6. Chartier L., Schiffrin E., Thibault G., Garcia R. Atrial natriuretic factor inhibits the stimulation of aldosterone secretion by angiotensin II, ACTH and potassium in vitro and angiotensin II-induced steroidogenesis in vivo. Endocrinology. 1984 Nov;115(5):2026–2028. doi: 10.1210/endo-115-5-2026. [DOI] [PubMed] [Google Scholar]
  7. De Léan A., Racz K., Gutkowska J., Nguyen T. T., Cantin M., Genest J. Specific receptor-mediated inhibition by synthetic atrial natriuretic factor of hormone-stimulated steroidogenesis in cultured bovine adrenal cells. Endocrinology. 1984 Oct;115(4):1636–1638. doi: 10.1210/endo-115-4-1636. [DOI] [PubMed] [Google Scholar]
  8. Kudo T., Baird A. Inhibition of aldosterone production in the adrenal glomerulosa by atrial natriuretic factor. Nature. 1984 Dec 20;312(5996):756–757. doi: 10.1038/312756a0. [DOI] [PubMed] [Google Scholar]
  9. Kuno T., Andresen J. W., Kamisaki Y., Waldman S. A., Chang L. Y., Saheki S., Leitman D. C., Nakane M., Murad F. Co-purification of an atrial natriuretic factor receptor and particulate guanylate cyclase from rat lung. J Biol Chem. 1986 May 5;261(13):5817–5823. [PubMed] [Google Scholar]
  10. Mukhopadhyay A. K., Bohnet H. G., Leidenberger F. A. Testosterone production by mouse Leydig cells is stimulated in vitro by atrial natriuretic factor. FEBS Lett. 1986 Jun 23;202(1):111–116. doi: 10.1016/0014-5793(86)80659-7. [DOI] [PubMed] [Google Scholar]
  11. Mukhopadhyay A. K., Bohnet H. G., Leidenberger F. A. Testosterone production by mouse Leydig cells is stimulated in vitro by atrial natriuretic factor. FEBS Lett. 1986 Jun 23;202(1):111–116. doi: 10.1016/0014-5793(86)80659-7. [DOI] [PubMed] [Google Scholar]
  12. Mukhopadhyay A. K., Schumacher M., Leidenberger F. A. Steroidogenic effect of atrial natriuretic factor in isolated mouse Leydig cells is mediated by cyclic GMP. Biochem J. 1986 Oct 15;239(2):463–467. doi: 10.1042/bj2390463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nakamura M., Odaguchi K., Shimizu T., Nakamura Y., Okamoto M. Stimulation of corticosterone production by atrial natriuretic polypeptide in hypophysectomized rats. Eur J Pharmacol. 1985 Nov 5;117(2):285–286. doi: 10.1016/0014-2999(85)90617-x. [DOI] [PubMed] [Google Scholar]
  14. Nambi P., Aiyar N. V., Sharma R. K. Adrenocorticotropin-dependent particulate guanylate cyclase in rat adrenal and adrenocortical carcinoma: comparison of its properties with soluble guanylate cyclase and its relationship with ACTH-induced steroidogenesis. Arch Biochem Biophys. 1982 Sep;217(2):638–646. doi: 10.1016/0003-9861(82)90545-8. [DOI] [PubMed] [Google Scholar]
  15. Nambi P., Aiyar N. V., Sharma R. K. Solubilization of epinephrine-specific alpha-adrenergic receptors from adrenocortical carcinoma. FEBS Lett. 1982 Apr 5;140(1):98–102. doi: 10.1016/0014-5793(82)80529-2. [DOI] [PubMed] [Google Scholar]
  16. Nambi P., Sharma R. K. Demonstration of ACTH-sensitive particulate guanylate cyclase in adrenocortical carcinoma. Biochem Biophys Res Commun. 1981 May 29;100(2):508–514. doi: 10.1016/s0006-291x(81)80206-9. [DOI] [PubMed] [Google Scholar]
  17. Naruse M., Obana K., Naruse K., Yamaguchi H., Demura H., Inagami T., Shizume K. Atrial natriuretic polypeptide inhibits cortisol secretion as well as aldosterone secretion in vitro from human adrenal tissue. J Clin Endocrinol Metab. 1987 Jan;64(1):10–16. doi: 10.1210/jcem-64-1-10. [DOI] [PubMed] [Google Scholar]
  18. Pandey K. N., Kovacs W. J., Inagami T. The inhibition of progesterone secretion and the regulation of cyclic nucleotides by atrial natriuretic factor in gonadotropin responsive murine Leydig tumor cells. Biochem Biophys Res Commun. 1985 Dec 17;133(2):800–806. doi: 10.1016/0006-291x(85)90975-1. [DOI] [PubMed] [Google Scholar]
  19. Pandey K. N., Pavlou S. N., Kovacs W. J., Inagami T. Atrial natriuretic factor regulates steroidogenic responsiveness and cyclic nucleotide levels in mouse Leydig cells in vitro. Biochem Biophys Res Commun. 1986 Jul 16;138(1):399–404. doi: 10.1016/0006-291x(86)90295-0. [DOI] [PubMed] [Google Scholar]
  20. Paul A. K., Marala R. B., Jaiswal R. K., Sharma R. K. Coexistence of guanylate cyclase and atrial natriuretic factor receptor in a 180-kD protein. Science. 1987 Mar 6;235(4793):1224–1226. doi: 10.1126/science.2881352. [DOI] [PubMed] [Google Scholar]
  21. Schwartz D., Geller D. M., Manning P. T., Siegel N. R., Fok K. F., Smith C. E., Needleman P. Ser-Leu-Arg-Arg-atriopeptin III: the major circulating form of atrial peptide. Science. 1985 Jul 26;229(4711):397–400. doi: 10.1126/science.3160114. [DOI] [PubMed] [Google Scholar]
  22. Sharma R. K., Ahmed N. K., Shanker G. Metabolic regulation of steroidogenesis in isolated adrenal cells of rat. Relationship of adrenocorticotropin-, adenosine 3':5'-monophosphate-and guanosine 3':5'-monophosphate-stimulated steroidogenesis with the activation of protein kinase. Eur J Biochem. 1976 Nov 15;70(2):427–433. doi: 10.1111/j.1432-1033.1976.tb11033.x. [DOI] [PubMed] [Google Scholar]
  23. Sharma R. K., Sawhney R. S. Metabolic regulation of steroidogenesis in isolated adrenal cell. Investigation of the adrenocorticotropic hormone, guanosine 3',5'-monophosphate, and adenosine 3',5'-monophosphate control step. Biochemistry. 1978 Jan 24;17(2):316–321. doi: 10.1021/bi00595a019. [DOI] [PubMed] [Google Scholar]
  24. Takayanagi R., Snajdar R. M., Imada T., Tamura M., Pandey K. N., Misono K. S., Inagami T. Purification and characterization of two types of atrial natriuretic factor receptors from bovine adrenal cortex: guanylate cyclase-linked and cyclase-free receptors. Biochem Biophys Res Commun. 1987 Apr 14;144(1):244–250. doi: 10.1016/s0006-291x(87)80502-8. [DOI] [PubMed] [Google Scholar]
  25. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Waldman S. A., Chang L. Y., Murad F. A two-step procedure for obtaining highly purified particulate guanylate cyclase from rat lung. Prep Biochem. 1985;15(3):103–119. doi: 10.1080/10826068508062265. [DOI] [PubMed] [Google Scholar]
  27. Winquist R. J., Faison E. P., Waldman S. A., Schwartz K., Murad F., Rapoport R. M. Atrial natriuretic factor elicits an endothelium-independent relaxation and activates particulate guanylate cyclase in vascular smooth muscle. Proc Natl Acad Sci U S A. 1984 Dec;81(23):7661–7664. doi: 10.1073/pnas.81.23.7661. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. de Bold A. J. Atrial natriuretic factor: a hormone produced by the heart. Science. 1985 Nov 15;230(4727):767–770. doi: 10.1126/science.2932797. [DOI] [PubMed] [Google Scholar]

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