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. 1988 Apr 15;251(2):541–545. doi: 10.1042/bj2510541

Palmitate inhibits liver glycolysis. Involvement of fructose 2,6-bisphosphate in the glucose/fatty acid cycle.

L Hue 1, L Maisin 1, M H Rider 1
PMCID: PMC1149034  PMID: 3401217

Abstract

In hepatocytes from overnight-fasted rats incubated with glucose, palmitate decreased the production of lactate, the detritiation of [2-3H]- and [3-3H]-glucose, and the concentration of fructose 2,6-bisphosphate. Similarly, perfusion of hearts from fed rats with beta-hydroxybutyrate resulted in an inhibition of the detritiation of [3-3H]glucose and a fall in fructose 2,6-bisphosphate concentration. This fall could result from an increase in citrate (hepatocytes and heart) and sn-glycerol 3-bisphosphate concentration. It is suggested that a fall in fructose 2,6-bisphosphate concentration participates in the inhibition of glycolysis by fatty acids and ketone bodies.

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Selected References

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  1. Bartrons R., Hue L., Van Schaftingen E., Hers H. G. Hormonal control of fructose 2,6-bisphosphate concentration in isolated rat hepatocytes. Biochem J. 1983 Sep 15;214(3):829–837. doi: 10.1042/bj2140829. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bontemps F., Hue L., Hers H. G. Phosphorylation of glucose in isolated rat hepatocytes. Sigmoidal kinetics explained by the activity of glucokinase alone. Biochem J. 1978 Aug 15;174(2):603–611. doi: 10.1042/bj1740603. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chen R. F. Removal of fatty acids from serum albumin by charcoal treatment. J Biol Chem. 1967 Jan 25;242(2):173–181. [PubMed] [Google Scholar]
  4. Declercq P. E., Debeer L. J., Mannaerts G. P. Role of glycerol 3-phosphate and glycerophosphate acyltransferase in the nutritional control of hepatic triacylglycerol synthesis. Biochem J. 1982 Apr 15;204(1):247–256. doi: 10.1042/bj2040247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. EVANS W. H., MUELLER P. S. EFFECTS OF PALMITATE ON THE METABOLISM OF LEUKOCYTES FROM GUINEA PIG EXUDATE. J Lipid Res. 1963 Jan;4:39–45. [PubMed] [Google Scholar]
  6. Garland P. B., Randle P. J. Regulation of glucose uptake by muscles. 10. Effects of alloxan-diabetes, starvation, hypophysectomy and adrenalectomy, and of fatty acids, ketone bodies and pyruvate, on the glycerol output and concentrations of free fatty acids, long-chain fatty acyl-coenzyme A, glycerol phosphate and citrate-cycle intermediates in rat heart and diaphragm muscles. Biochem J. 1964 Dec;93(3):678–687. doi: 10.1042/bj0930678. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hems R., Lund P., Krebs H. A. Rapid separation of isolated hepatocytes or similar tissue fragments for analysis of cell constituents. Biochem J. 1975 Jul;150(1):47–50. doi: 10.1042/bj1500047. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hue L., Felíu J. E., Hers H. G. Control of gluconeogenesis and of enzymes of glycogen metabolism in isolated rat hepatocytes. A parallel study of the effect of phenylephrine and of glucagon. Biochem J. 1978 Dec 15;176(3):791–797. doi: 10.1042/bj1760791. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hue L., Rider M. H. Role of fructose 2,6-bisphosphate in the control of glycolysis in mammalian tissues. Biochem J. 1987 Jul 15;245(2):313–324. doi: 10.1042/bj2450313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hue L. The role of futile cycles in the regulation of carbohydrate metabolism in the liver. Adv Enzymol Relat Areas Mol Biol. 1981;52:247–331. doi: 10.1002/9780470122976.ch4. [DOI] [PubMed] [Google Scholar]
  11. Laloux M., Van Schaftingen E., Francois J., Hers H. G. Phosphate dependency of phosphofructokinase 2. Eur J Biochem. 1985 Apr 1;148(1):155–159. doi: 10.1111/j.1432-1033.1985.tb08819.x. [DOI] [PubMed] [Google Scholar]
  12. Mowbray J., Ottaway J. H. The flux of pyruvate in perfused rat heart. Eur J Biochem. 1973 Jul 16;36(2):362–368. doi: 10.1111/j.1432-1033.1973.tb02920.x. [DOI] [PubMed] [Google Scholar]
  13. Randle P. J., Garland P. B., Hales C. N., Newsholme E. A., Denton R. M., Pogson C. I. Interactions of metabolism and the physiological role of insulin. Recent Prog Horm Res. 1966;22:1–48. doi: 10.1016/b978-1-4831-9825-5.50004-x. [DOI] [PubMed] [Google Scholar]
  14. Randle P. J., Newsholme E. A., Garland P. B. Regulation of glucose uptake by muscle. 8. Effects of fatty acids, ketone bodies and pyruvate, and of alloxan-diabetes and starvation, on the uptake and metabolic fate of glucose in rat heart and diaphragm muscles. Biochem J. 1964 Dec;93(3):652–665. doi: 10.1042/bj0930652. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rider M. H., Hue L. Activation of rat heart phosphofructokinase-2 by insulin in vivo. FEBS Lett. 1984 Oct 29;176(2):484–488. doi: 10.1016/0014-5793(84)81223-5. [DOI] [PubMed] [Google Scholar]
  16. Rider M. H., Hue L. Regulation of fructose 2,6-bisphosphate concentration in white adipose tissue. Biochem J. 1985 Jan 15;225(2):421–428. doi: 10.1042/bj2250421. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ross B. D., Hems R., Krebs H. A. The rate of gluconeogenesis from various precursors in the perfused rat liver. Biochem J. 1967 Mar;102(3):942–951. doi: 10.1042/bj1020942. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Scrutton M. C., Utter M. F. Pyruvate carboxylase. IX. Some properties of the activation by certain acyl derivatives of coenzyme A. J Biol Chem. 1967 Apr 25;242(8):1723–1735. [PubMed] [Google Scholar]
  19. Siess E. A., Brocks D. G., Lattke H. K., Wieland O. H. Effect of glucagon on metabolite compartmentation in isolated rat liver cells during gluconeogenesis from lactate. Biochem J. 1977 Aug 15;166(2):225–235. doi: 10.1042/bj1660225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Struck E., Ashmore J., Wieland O. Stimulierung der Gluconeogenese durch langkettige Fettsäuren und Glucagon. Biochem Z. 1965 Nov 5;343(1):107–110. [PubMed] [Google Scholar]
  21. Tippett P. S., Neet K. E. Specific inhibition of glucokinase by long chain acyl coenzymes A below the critical micelle concentration. J Biol Chem. 1982 Nov 10;257(21):12839–12845. [PubMed] [Google Scholar]
  22. Van Schaftingen E. Fructose 2,6-bisphosphate. Adv Enzymol Relat Areas Mol Biol. 1987;59:315–395. doi: 10.1002/9780470123058.ch7. [DOI] [PubMed] [Google Scholar]
  23. Van Schaftingen E., Hers H. G. Purification and properties of phosphofructokinase 2/fructose 2,6-bisphosphatase from chicken liver and from pigeon muscle. Eur J Biochem. 1986 Sep 1;159(2):359–365. doi: 10.1111/j.1432-1033.1986.tb09876.x. [DOI] [PubMed] [Google Scholar]
  24. Van Schaftingen E., Hue L., Hers H. G. Extracellular metabolites in suspensions of isolated hepatocytes. Biochem J. 1987 Dec 1;248(2):517–521. doi: 10.1042/bj2480517. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. WILLIAMSON J. R., KREBS H. A. Acetoacetate as fuel of respiration in the perfused rat heart. Biochem J. 1961 Sep;80:540–547. doi: 10.1042/bj0800540. [DOI] [PMC free article] [PubMed] [Google Scholar]

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